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  1. 8281
    “…The genomic and post-transcriptional alterations of TROAP in tumors were determined using the cBioPortal and SangerBox databases. Also, TISIDB was used to investigate the relationship between TROAP expression and tumor microenvironment(TME) among different cancer types. …”
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    Pan-cancer analysis of trophinin-associated protein with potential implications in clinical significance, prognosis, and tumor microenvironment in human cancers
  2. 8282
    “…All samples were cultured in a selective Lucie Bardet Jean-Marc Rolain (LBJMR) medium and colonies were identified using MALDI-TOF mass spectrometry and then antibiotic susceptibility tests were performed. PCR and Sanger sequencing were performed to screen for the presence of mcr genes. …”
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    Screening of colistin-resistant bacteria in livestock animals from France
  3. 8283
    “…Using the predictions in silico tools, the candidate variants were segregated by Sanger sequencing. Based on the risk of being damaging and on the potential role in rotator cuff etiopathogenesis, three candidate genes for rotator cuff tears were prioritized: COL23A1, EMILIN3 and HDAC10. …”
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    Genetic Characterization in Familial Rotator Cuff Tear: An Exome Sequencing Study
  4. 8284
    “…METHODS: Whole exome and Sanger sequencing was performed in two IBD patients. …”
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    RIPK1 mutations causing infantile-onset IBD with inflammatory and fistulizing features
  5. 8285
    “…Next-generation sequencing (NGS) and Sanger sequencing further demonstrated a heterozygous nonsense variant (exon16, c.G1985A: p.W662X) in SLC4A1, inherited from his father. …”
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    A novel variant of SLC4A1 for hereditary spherocytosis in a Chinese family: a case report and systematic review
  6. 8286
    “…PrimerScore2 was also used to design 77 monoplex Sanger sequencing primers, the sequencing results indicated that all the primers were effective.…”
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    High-throughput primer design by scoring in piecewise logistic model for multiple polymerase chain reaction variants
  7. 8287
    “…METHODS: Whole exome sequencing (WES) was performed on all patients and their parents, followed by Sanger sequencing validation. Bioinformatics tools were used to provide supporting evidence for pathogenicity of variants. …”
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    Whole exome sequencing identifies two novel variants in PHEX and DMP1 in Malaysian children with hypophosphatemic rickets
  8. 8288
    “…The full polyprotein-encoding genes were retrieved by a combination of high throughput and Sanger sequencing from 17 samples showing sufficiently high viral loads. …”
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    Association of hepatitis C virus genotype 2 spread with historic slave trade and commerce routes in Western Africa
  9. 8289
    “…In this study, we conducted Sanger sequencing of formalin-fixed paraffin-embedded (FFPE) diagnostic tumor samples using a target-panel to search for recurrent mutations involving the IDH-1/IDH-2, TET-2, DNMT3A and RhoA genes in 59 cases of nMTCL. …”
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    Tumor mutation burden involving epigenetic regulatory genes and the RhoA GTPase predicts overall survival in nodal mature T-cell lymphomas
  10. 8290
    “…Methods: The characterization of circ_RNF13 was determined by Sanger sequencing, qRT-PCR, subcellular fractionation assay, and RNA FISH. …”
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    Circ_RNF13 Regulates the Stemness and Chemosensitivity of Colorectal Cancer by Transcriptional Regulation of DDX27 Mediated by TRIM24 Stabilization
  11. 8291
    “…The NGS sequence of the sheep‐derived MERS‐CoV was confirmed by conventional RT‐PCR of a part of the N gene and subsequent Sanger sequencing. All MERS‐CoV sequences clustered within clade B, lineage 5. …”
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    MERS‐CoV in sheep, goats, and cattle, United Arab Emirates, 2019: Virological and serological investigations reveal an accidental spillover from dromedaries
  12. 8292
    “…The open reading frames (ORF)5 represents approximately 4% of the porcine reproductive and respiratory syndrome virus (PRRSV)‐2 genome (whole‐PRRSV) and is often determined by the Sanger technique, which rarely detects >1 PRRSV strain if present in the sample. …”
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    Implementing a user‐friendly format to analyze PRRSV next‐generation sequencing results and associating breeding herd production performance with number of PRRSV strains and recombination events
  13. 8293
    “…PIK3CA (exon 9 and 20) and RAS ( KRAS & NRAS ) was performed on representative formalin-fixed paraffin-embedded tissues by Sanger sequencing. Results were correlated with clinicopathological features. …”
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    The Prevalence of BRAF, PIK3CA, and RAS Mutations in Indian Patients with Colorectal Cancer
  14. 8294
    “…Although rare, compound heterozygous mutations also occur, with the two variants on different alleles (NUDT15*3/*6), which may confer tolerance to considerably lesser mercaptopurine dosage. Sanger sequencing or pyrosequencing can determine the NUDT15 variants but not the phase. …”
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    Allele-specific polymerase chain reaction can determine the diplotype of NUDT15 variants in patients with childhood acute lymphoblastic Leukemia
  15. 8295
    “…METHODS: Viral whole genome and partial S-segment sequencing was performed using Illumina and Sanger platforms, respectively and lineage assignemnt was assessed using Pangolin version 1.2.4 and scorpio version 3.4.X. …”
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    The Delta variant wave in Tunisia: Genetic diversity, spatio-temporal distribution and evidence of the spread of a divergent AY.122 sub-lineage
  16. 8296
    “…Isolates with cefepime-taniborbactam MICs of ≥16 μg/mL underwent whole-genome sequencing. β-lactamase genes were identified in meropenem-resistant isolates by PCR/Sanger sequencing. Against Enterobacterales, taniborbactam reduced the cefepime MIC(90) value by >64-fold (from >16 to 0.25 μg/mL). …”
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    In Vitro Activity of Cefepime-Taniborbactam and Comparators against Clinical Isolates of Gram-Negative Bacilli from 2018 to 2020: Results from the Global Evaluation of Antimicrobial Resistance via Surveillance (GEARS) Program
  17. 8297
    “…Bioinformatic tools were used to identify SVs from the long sequencing reads, with confirmation of findings in probands by short-read Illumina sequencing, Sanger sequencing and visual manual validation with Integrated Genome Browser. …”
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    Third-generation genome sequencing implicates medium-sized structural variants in chronic schizophrenia
  18. 8298
    “…He was evaluated by renal biopsy,whole-exome sequencing (WES) and whole-genome sequencing (WGS) for Alport syndrome. RT-PCR and cDNA Sanger sequencing were performed on RNA extracted from the skin of the proband. …”
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    A deep intronic splice variant of the COL4A5 gene in a Chinese family with X-linked Alport syndrome
  19. 8299
    “…METHODS: Using PCR protocols and Sanger sequencing, we assessed the presence of Anaplasma spp. in blood and ticks collected on a native deer species (Pudu puda) from southern Chile. …”
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    Wild deer (Pudu puda) from Chile harbor a novel ecotype of Anaplasma phagocytophilum
  20. 8300
    “…Our data revealed wide prevalence of the then widespread through Europe B.1.177 variant, although some viral samples could not be classified with the limited Sanger sequencing tools used. A particularly interesting sample could not be fitted to a major variant then circulating in Europe and has been subjected here to full genome sequencing in an attempt to clarify its origin, lineage and relations with the seven full genome sequences deposited for that period in Sicily, hoping to provide clues on viral evolution. …”
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    A SARS-CoV-2 full genome sequence of the B.1.1 lineage sheds light on viral evolution in Sicily in late 2020
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