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  1. 9161
    “…Plasmid-mediated quinolone resistance (PMQR) genes were detected by PCR amplification and quinolone resistance-determining regions (QRDRs) of the gyrA and parC genes were analyzed following Sanger sequencing of the PCR products. Thirty-four Salmonella strains with reduced susceptibility to fluoroquinolones (ciprofloxacin MIC ≥ 0.125 µg/mL and levofloxacin MIC ≥ 0.25 µg/mL) were selected from 208 human clinical Salmonella isolates. …”
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    Presence of plasmid-mediated quinolone resistance (PMQR) genes in non-typhoidal Salmonella strains with reduced susceptibility to fluoroquinolones isolated from human salmonellosis in Gyeonggi-do, South Korea from 2016 to 2019
  2. 9162
    “…A T7 endonuclease I (T7EI) assay was performed on flow cytometry enriched transfected cells in order to detect CRISPR-mediated targeted mutations in the cyp1a1 locus, revealing an overall gene editing efficiency of 39%. Sanger sequencing coupled with bioinformatic analysis led to the detection of multiple insertions and deletions of variable lengths in the cyp1a1 region directed by CRISPR/Cas9 machinery. …”
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    A ribonucleoprotein transfection strategy for CRISPR/Cas9‐mediated gene editing and single cell cloning in rainbow trout cells
  3. 9163
    “…Because a non-synonymous mutation (c.642,923 G > A, P265L) changed DIO2 protein secondary structure in knob geese, and Sanger sequencing further showed that the AA genotype was identified in the population of knob geese, and was prevalent in a crossing population which was artificially selected for 10 generations. …”
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    Integrative analysis of histomorphology, transcriptome and whole genome resequencing identified DIO2 gene as a crucial gene for the protuberant knob located on forehead in geese
  4. 9164
    “…Selected potentially pathogenic variants were confirmed using Sanger sequencing methods and evaluated by the American College of Medical Genetics (ACMG) standards and guidelines. …”
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    Growth Plate Genes Are Key Regulators of Growth: Lessons Learned From Children of Consanguineous Families From Kurdistan, Iraq
  5. 9165
    “…Variants were confirmed using Sanger sequencing and predicted splice-site mutations were investigated using an in vitro splicing assay. …”
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    In Vitro Splicing Assay Proves the Pathogenicity of Intronic Variants in MRAP
  6. 9166
    “…Subgingival plaque and blood samples were obtained from both groups and were subjected to further molecular analysis for NLRP3 (rs4612666) and CARD8 (rs2043211) expression and allele change using conventional polymerase chain reaction (PCR) and gene sequencing (Sanger’s method). Results: Amongst the demographic variables, age, and monthly income were statistically significant between the two groups. …”
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    Genetic Polymorphisms of NLRP3 (rs4612666) and CARD8 (rs2043211) in Periodontitis and Cardiovascular Diseases
  7. 9167
    “…A CRISPR/Cas9 editing pipeline was developed for KO of Ndrg2 in DCs with a transfection rate and editing efficiency of > 90% shown by Sanger Sequencing. Excisional wounds treated with Ndrg2-KO DCs demonstrated significantly accelerated healing compared to control DCs and blank hydrogels. scRNA seq revealed that Ndrg2 downregulation strongly induced Vegfa expression and anti-oxidant transcriptomic signatures. …”
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    QS3: CRISPR/Cas9 Editing of Autologous Dendritic Cells to Enhance Angiogenesis and Wound Healing
  8. 9168
    “…We suspected that she was suffering from GS by initial estimation. Direct Sanger sequencing was used to analyze the causative gene SLC12A3 of GS. …”
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    A novel compound heterozygous variant of the SLC12A3 gene in Gitelman syndrome with diabetes and the choices of the appropriate hypoglycemic drugs: a case report
  9. 9169
    “…We validated the methylation state and expression levels of candidate genes in each cellular phenotype through Sanger sequencing and reverse transcription polymerase chain reaction, respectively. …”
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    Transcriptional epigenetic regulation of Fkbp1/Pax9 genes is associated with impaired sensitivity to platinum treatment in ovarian cancer
  10. 9170
    “…The HIV-1 pol region was amplified by reverse transcriptional PCR and sequenced by sanger sequencing. Genotypes, surveillance drug resistance mutations (SDRMs) and TDR were analyzed. …”
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    Transmitted drug resistance and transmission clusters among HIV-1 treatment-naïve patients in Guangdong, China: a cross-sectional study
  11. 9171
    “…The proband and her parents were screened for the responsible mutation using second-generation whole exon sequencing, and the members of the family were verified for suspected mutations using Sanger sequencing. Mutant and wild type plasmids were constructed and transfected into HEK293T cells to detect the mRNA and protein expression of PROS1. …”
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    A thrombophilia family with protein S deficiency due to protein translation disorders caused by a Leu607Ser heterozygous mutation in PROS1
  12. 9172
    “…Additionally, non-CDS mutations in TNFAIP3 in 41 TCL patients from our center (JNU) were investigated by polymerase chain reaction (PCR) and Sanger sequencing. Furthermore, non-CDS mutations in TNFAIP3 in 47 TCL patients from Gene Expression Omnibus (GEO) dataset were explored. …”
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    TNFAIP3 mutation may be associated with favorable overall survival for patients with T-cell lymphoma
  13. 9173
    “…HLA‐A alleles were typed using Sanger sequencing‐based typing techniques with DNA extracted from the peripheral blood. …”
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    HPV16 E6-specific T cell response and HLA-A alleles are related to the prognosis of patients with cervical cancer
  14. 9174
    por Garam, Nóra, Cserhalmi, Marcell, Prohászka, Zoltán, Szilágyi, Ágnes, Veszeli, Nóra, Szabó, Edina, Uzonyi, Barbara, Iliás, Attila, Aigner, Christof, Schmidt, Alice, Gaggl, Martina, Sunder-Plassmann, Gere, Bajcsi, Dóra, Brunner, Jürgen, Dumfarth, Alexandra, Cejka, Daniel, Flaschberger, Stefan, Flögelova, Hana, Haris, Ágnes, Hartmann, Ágnes, Heilos, Andreas, Mueller, Thomas, Rusai, Krisztina, Arbeiter, Klaus, Hofer, Johannes, Jakab, Dániel, Sinkó, Mária, Szigeti, Erika, Bereczki, Csaba, Janko, Viktor, Kelen, Kata, Reusz, György S., Szabó, Attila J., Klenk, Nóra, Kóbor, Krisztina, Kojc, Nika, Knechtelsdorfer, Maarten, Laganovic, Mario, Lungu, Adrian Catalin, Meglic, Anamarija, Rus, Rina, Kersnik Levart, Tanja, Macioniene, Ernesta, Miglinas, Marius, Pawłowska, Anna, Stompór, Tomasz, Podracka, Ludmila, Rudnicki, Michael, Mayer, Gert, Rysava, Romana, Reiterova, Jana, Saraga, Marijan, Seeman, Tomáš, Zieg, Jakub, Sládková, Eva, Stajic, Natasa, Szabó, Tamás, Capitanescu, Andrei, Stancu, Simona, Tisljar, Miroslav, Galesic, Kresimir, Tislér, András, Vainumäe, Inga, Windpessl, Martin, Zaoral, Tomas, Zlatanova, Galia, Józsi, Mihály, Csuka, Dorottya
    Publicado 2021
    “…FHR-5 serum levels were measured in ELISA, the CFHR5 gene was analyzed by Sanger sequencing, and selected variants were studied as recombinant proteins in ELISA and surface plasmon resonance (SPR). …”
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    FHR-5 Serum Levels and CFHR5 Genetic Variations in Patients With Immune Complex-Mediated Membranoproliferative Glomerulonephritis and C3-Glomerulopathy
  15. 9175
    “…To validate fusion transcripts, reverse transcription-PCR was performed on 16 candidates, with an accuracy reaching up to 87.5%. Sanger sequencing of the PCR products further confirmed the authenticity of chimeric transcripts. …”
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    Full-length transcriptome analysis and identification of transcript structures in Eimeria necatrix from different developmental stages by single-molecule real-time sequencing
  16. 9176
    “…Exonuclease digestion assay, actinomycin D, agarose gel electrophoresis (AGE), and Sanger sequencing verified the potential of hsa_circ_0007507 as a biomarker. …”
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    Circular RNA hsa_circ_0007507 May Serve as a Biomarker for the Diagnosis and Prognosis of Gastric Cancer
  17. 9177
    “…Samples from patients showing no abnormalities in MS-MLPA or bisulfite pyrosequencing were analyzed by single nucleotide polymorphism (SNP) microarray and CDKN1C Sanger sequencing. We introduced a metric named as the methylation difference, defined as the difference in DNA methylation levels between DMR1 and DMR2. …”
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    Prospective study of epigenetic alterations responsible for isolated hemihyperplasia/hemihypoplasia and their association with leg length discrepancy
  18. 9178
    “…Samples that were positive for P. falciparum were genotyped for the pfk13 gene using the Sanger sequencing method. The single nucleotide polymorphisms were analysed using the Bioedit software. …”
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    Absence of Plasmodium falciparum artemisinin resistance gene mutations eleven years after the adoption of artemisinin-based combination therapy in Nigeria
  19. 9179
    “…METHODS: Two different cohorts of breast cancer cases were investigated, the Alabama Hereditary Cancer Cohort and The Cancer Genome Atlas, which were analyzed through nested PCRs/Sanger sequencing and whole-exome sequencing, respectively. …”
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    Rare and potentially pathogenic variants in hydroxycarboxylic acid receptor genes identified in breast cancer cases
  20. 9180
    “…The 130 NSA isolates were represented by 16 bacterial genera, of which 97% were identified as spore-formers via Sanger sequencing. The most spatially abundant isolate was Bacillus subtilis, and the most temporally abundant spore-former was Virgibacillus panthothenticus. …”
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    Clean room microbiome complexity impacts planetary protection bioburden
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