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Mostrando 121 - 140 Resultados de 9,396 Para Buscar '"Sanger"', tiempo de consulta: 0.31s Limitar resultados
  1. 121
    por Lee, Sin Hang
    Publicado 2021
    “…This protocol was able to amplify both nucleic acid targets into cDNA amplicons to be used as templates for Sanger sequencing on all 16 clinical specimens that were positive for SARS-CoV-2.…”
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    Online Artículo Texto
    A Routine Sanger Sequencing Target Specific Mutation Assay for SARS-CoV-2 Variants of Concern and Interest
  2. 122
    “…We analyzed the nucleotide sequence of the Receptor Binding Domain (RBD) region of SARS-CoV-2 by the Sanger sequencing using in-house developed methods. …”
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    Online Artículo Texto
    Genomic surveillance of SARS-CoV-2 by sequencing the RBD region using Sanger sequencing from North Kerala
  3. 123
    “…METHODS: Overall, 80 samples with LLV were amplified and sequenced using a commercial Sanger sequencing (SS) genotyping kit. Furthermore, 51 samples successfully sequenced using SS were simultaneously subjected to NGS. …”
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    Online Artículo Texto
    HIV-1 Genotypic Resistance Testing Using Sanger and Next-Generation Sequencing in Adults with Low-Level Viremia in China
  4. 124
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    Pauli Obituary Collection: "Wolfgang Pauli-Vorlesungen des Wintersemesters 1972-73: F. Sanger, The chemistry of living matter", announcement
  5. 125
    “…Here, we investigated a cluster of SARS-CoV-2 cases by targeted Sanger sequencing and clinical epidemiological methods in a non-COVID-19 super-specialty hospital. …”
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    Online Artículo Texto
    Targeted Sanger Sequencing of a Cluster of COVID-19 Cases in the Surgical ICU of a Non-COVID Hospital: Lessons Learned
  6. 126
    “…Within the range between 0 and 20% methylation, however, Sanger sequencing data have to be interpreted cautiously, at least in the investigated region of interest (TRPA1 promotor region). …”
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    Online Artículo Texto
    Comparison of methylation estimates obtained via MinION nanopore sequencing and sanger bisulfite sequencing in the TRPA1 promoter region
  7. 127
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    A sweetpotato gene index established by de novo assembly of pyrosequencing and Sanger sequences and mining for gene-based microsatellite markers
  8. 128
    “…Frequency of EGFR, K-Ras, B-Raf, PIK3CA, DDR2, ALK, and PDGFRA gene mutations was examined in 56 patients using next-generation sequencing, polymerase chain reaction, and Sanger sequencing. Macrodissection or microdissection was performed in 37 cases to separate the adenomatous and squamous components of ASC. …”
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    Online Artículo Texto
    Screening for major driver oncogene alterations in adenosquamous lung carcinoma using PCR coupled with next-generation and Sanger sequencing methods
  9. 129
    “…RESULTS: We compared Sanger and next generation sequencing to validate microRNAs associated with diabetic kidney disease identified from an epigenome-wide association study (EWAS). …”
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    Online Artículo Texto
    Validation of differentially methylated microRNAs identified from an epigenome-wide association study; Sanger and next generation sequencing approaches
  10. 130
    “…Although it is a commonly used method, Sanger sequencing has several limitations in detecting the BRAF (V600E) mutation. …”
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    Online Artículo Texto
    Comparison of droplet digital PCR and direct Sanger sequencing for the detection of the BRAF (V600E) mutation in papillary thyroid carcinoma
  11. 131
    “…The specificity of the qPCR gyrA followed by Sanger sequencing was 100%, indicating that the best sequence identity was always H. pylori. …”
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    Online Artículo Texto
    Comparison of the Diagnostic Performance of qPCR, Sanger Sequencing, and Whole-Genome Sequencing in Determining Clarithromycin and Levofloxacin Resistance in Helicobacter pylori
  12. 132
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    Online Artículo Texto
    Hemizygous amplification and complete Sanger sequencing of HLA‐C*07:37:01:02 from a South European Caucasoid
  13. 133
    “…Despite the improved accuracy of next-generation sequencing (NGS), it is widely accepted that variants need to be validated using Sanger sequencing before reporting. Validation of all NGS variants considerably increases the turnaround time and costs of clinical diagnosis. …”
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    Online Artículo Texto
    Sanger sequencing is no longer always necessary based on a single-center validation of 1109 NGS variants in 825 clinical exomes
  14. 134
    “…Sanger sequencing of POU3F4 was carried out for these IP-III patients. …”
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    Online Artículo Texto
    Genetic findings of Sanger and nanopore single-molecule sequencing in patients with X-linked hearing loss and incomplete partition type III
  15. 135
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    Online Artículo Texto
    Fast detection of SARS-CoV-2 variants including Omicron using one-step RT-PCR and Sanger sequencing
  16. 136
    “…The SARS-CoV-2 genome consists of approximately 30 kb single-stranded RNA that is too large to analyze the whole genome by Sanger sequencing. Thus, in this study, we performed Sanger sequencing following long-range RT-PCR of the entire SARS-CoV-2 S-gene and analyzed the mutational dynamics. …”
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    Online Artículo Texto
    Tracking SARS-CoV-2 variants by entire S-gene analysis using long-range RT-PCR and Sanger sequencing
  17. 137
    “…Here we explored the intra‐genomic diversity of Nassellaria and Spumellaria specimens (Radiolaria) by comparing Sanger sequencing with Illumina and Oxford Nanopore Technologies (MinION). …”
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    Online Artículo Texto
    Intra‐genomic rRNA gene variability of Nassellaria and Spumellaria (Rhizaria, Radiolaria) assessed by Sanger, MinION and Illumina sequencing
  18. 138
    “…The identification results of MALDI-TOF MS and 16S rRNA gene Sanger sequencing were almost identical at species level (66.7%; 64.3%). …”
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    Online Artículo Texto
    Comparing the Efficacy of MALDI-TOF MS and Sequencing-Based Identification Techniques (Sanger and NGS) to Monitor the Microbial Community of Irrigation Water
  19. 139
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    Online Artículo Texto
    A Genotypic Test for HIV-1 Tropism Combining Sanger Sequencing with Ultradeep Sequencing Predicts Virologic Response in Treatment-Experienced Patients
  20. 140
    “…Here, we determine how a 5-locus Sanger dataset compares with a 377-locus anchored genomics dataset for understanding the evolutionary history of the pseudoxyrhophiine snake radiation centered in Madagascar. …”
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    Comparing species tree estimation with large anchored phylogenomic and small Sanger-sequenced molecular datasets: an empirical study on Malagasy pseudoxyrhophiine snakes
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