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1361por Suo, Daqin, Wang, Ling, Zeng, Tingting, Zhang, Hui, Li, Lei, Liu, Jinyun, Yun, Jingping, Guan, Xin-Yuan, Li, Yan“…Here, we report that NRIP3 (nuclear receptor interacting protein 3) promotes ESCC tumor cell growth and resistance to CRT in ESCC cells by increasing and binding to DDI1 (DNA-damage inducible 1 homolog 1) and RTF2 (homologous to Schizosaccharomyces pombe Rtf2), and accelerating the removal of RTF2, which is a key determinant for the ability of cells to manage replication stress. …”
Publicado 2020
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1362por Batubara, Irmanida, Astuti, Rika Indri, Prastya, Muhammad Eka, Ilmiawati, Auliya, Maeda, Miwa, Suzuki, Mayu, Hamamoto, Akie, Takemori, Hiroshi“…Methods: In addition to in vitro assays, we monitored antioxidative and antiaging signals in Schizosaccharomyces pombe (yeast) and mouse melanoma B16F10 cells. …”
Publicado 2020
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1363por Pataki, Emese, Simhaev, Luba, Engel, Hamutal, Cohen, Adiel, Kupiec, Martin, Weisman, Ronit“…In the fission yeast, Schizosaccharomyces pombe, TORC2 and its target, the AGC kinase Gad8 (an orthologue of human AKT or SGK1) are required for viability under stress conditions and for developmental processes in response to starvation cues. …”
Publicado 2020
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1364por Torres-Garcia, Sito, Di Pompeo, Lorenza, Eivers, Luke, Gaborieau, Baptiste, White, Sharon A., Pidoux, Alison L., Kanigowska, Paulina, Yaseen, Imtiyaz, Cai, Yizhi, Allshire, Robin C.“…Current CRISPR/Cas9 systems for the fission yeast Schizosaccharomyces pombe rely on tedious and time-consuming cloning procedures to introduce a specific sgRNA target sequence into a Cas9-expressing plasmid. …”
Publicado 2020
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1365“…Fsv1/Stx8 is a Schizosaccharomyces pombe protein similar to mammalian syntaxin 8. stx8Δ cells are sensitive to salts, and the prevacuolar endosome (PVE) is altered in stx8Δ cells. …”
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1366por Loke, Steffen, Stoll, Anna, Machalz, David, Botrè, Francesco, Wolber, Gerhard, Bureik, Matthias, Parr, Maria Kristina“…To investigate the steric and regional requirements of substrates for this enzyme, we performed whole-cell biotransformation assays using a strain of fission yeast Schizosaccharomyces pombe recombinantly expressing CYP21A2. …”
Publicado 2021
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1367por Escott, Carlos, López, Carmen, Loira, Iris, González, Carmen, Bañuelos, María Antonia, Tesfaye, Wendu, Suárez-Lepe, José Antonio, Morata, Antonio“…In this experimental set up, Tempranillo grapes were subjected to pulsed light treatment, and the fermentative performance of non-Saccharomyces yeasts belonging to the species Schizosaccharomyces pombe, Lachancea thermotolerans, Torulaspora delbrueckii, Metschnikowia pulcherrima and Hanseniaspora vineae was monitored in sequential fermentations against spontaneous fermentation and pure culture fermentation with the species Saccharomyces cerevisiae. …”
Publicado 2021
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1368por Lewis, Richard Alexander, Devi, Jenileima, Green, Katherine, Li, Juanjuan, Hopkins, Adam, Hayles, Jacqueline, Nurse, Paul, Errington, Jeff, Allenby, Nicholas Edward Ellis“…This study was designed to identify and investigate bioactive natural product compounds that alter the cellular shape of the fission yeast Schizosaccharomyces pombe and induce a “rounded” or “small” cellular morphological phenotype. …”
Publicado 2021
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1369por Soriano, Ignacio, Vazquez, Enrique, De Leon, Nagore, Bertrand, Sibyl, Heitzer, Ellen, Toumazou, Sophia, Bo, Zhihan, Palles, Claire, Pai, Chen-Chun, Humphrey, Timothy C., Tomlinson, Ian, Cotterill, Sue, Kearsey, Stephen E.“…To understand better the contribution of hypermutagenesis to tumour development, we have modelled the most recurrent POLE-EDM (POLE-P286R) in Schizosaccharomyces pombe. Whole-genome sequencing analysis revealed that the corresponding pol2-P287R allele also has a strong mutator effect in vivo, with a high frequency of base substitutions and relatively few indel mutations. …”
Publicado 2021
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1370“…This review highlights recent knowledge on the function of fungal orthologs of the Schizosaccharomyces pombe Atf1, Aspergillus nidulans AtfA, and Fusarium verticillioides FvAtfA, bZIP-type transcription factors with a special focus on pathogenic species. …”
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1371por Ellis, David A., Reyes-Martín, Félix, Rodríguez-López, María, Cotobal, Cristina, Sun, Xi-Ming, Saintain, Quentin, Jeffares, Daniel C., Marguerat, Samuel, Tallada, Víctor A., Bähler, Jürg“…To characterize genome rearrangements during cellular ageing in non-dividing cells, we interrogated a single diagnostic measure, DNA breakpoint junctions, using Schizosaccharomyces pombe as a model system. Aberrant DNA junctions that accumulated with age were associated with microhomology sequences and R-loops. …”
Publicado 2021
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1372por Qu, Yingmin, Wang, Jing, Huang, Pengyun, Liu, Xiaohong, Lu, Jianping, Lin, Fu-Cheng“…Here, we identified PoRal2, a homologous protein of Schizosaccharomyces pombe Ral2, and characterized its roles in fungal development and virulence in P. oryzae. …”
Publicado 2021
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1373por Nagakubo, Toshiki, Yamamoto, Tatsuya, Asamizu, Shumpei, Toyofuku, Masanori, Nomura, Nobuhiko, Onaka, Hiroyasu“…Using co-culture method, we found that colonies of SLP-deficient mutants of S. lividans were more severely invaded by fungi, including Saccharomyces cerevisiae and Schizosaccharomyces pombe. In addition, microscopic and transcriptional analyses demonstrated that SLP expression was elevated upon co-culture with the fungi. …”
Publicado 2021
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1374por Vo, Jenny Mai, Mulroney, Logan, Quick-Cleveland, Jen, Jain, Miten, Akeson, Mark, Ares, Manuel“…We tested two 3′ nucleotidyl transferases, Saccharomyces cerevisiae poly(A) polymerase and Schizosaccharomyces pombe Cid1, for the ability to add base and sugar modified rNTPs to free RNA 3′ ends, eventually focusing on Cid1. …”
Publicado 2021
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1375por Vaisman, Alexandra, McDonald, John P., Smith, Mallory R., Aspelund, Sender L., Evans, Thomas C., Woodgate, Roger“…Humans possess all four Y-family pols (pols η, ι, κ, and Rev1), Schizosaccharomyces pombe has three Y-family pols (pols η, κ, and Rev1), and Saccharomyces cerevisiae only has polη and Rev1. …”
Publicado 2021
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1376por Rodriguez-Lopez, Maria, Anver, Shajahan, Cotobal, Cristina, Kamrad, Stephan, Malecki, Michal, Correia-Melo, Clara, Hoti, Mimoza, Townsend, StJohn, Marguerat, Samuel, Pong, Sheng Kai, Wu, Mary Y, Montemayor, Luis, Howell, Michael, Ralser, Markus, Bähler, Jürg“…To explore this question, we systematically analysed cellular roles of lincRNAs in Schizosaccharomyces pombe. Using seamless CRISPR/Cas9-based genome editing, we deleted 141 lincRNA genes to broadly phenotype these mutants, together with 238 diverse coding-gene mutants for functional context. …”
Publicado 2022
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1377por Lee, Sol, Nam, Miyoung, Lee, Ah-Reum, Baek, Seung-Tae, Kim, Min Jung, Kim, Ju Seong, Kong, Andrew Hyunsoo, Lee, Minho, Lee, Sook-Jeong, Kim, Seon-Young, Kim, Dong-Uk, Hoe, Kwang-Lae“…To achieve a better understanding of the ER-independent antifungal action mechanisms of TAM, we systematically identified TAM-sensitive genes through microarray screening of the heterozygous gene deletion library in fission yeast (Schizosaccharomyces pombe). Secondary confirmation was followed by a spotting assay, finally yielding 13 TAM-sensitive genes under the drug-induced haploinsufficient condition. …”
Publicado 2021
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1378“…Pre-mRNA processing factor 4 kinase (PRP4K, also known as PRPF4B) is an essential kinase first identified in the fission yeast Schizosaccharomyces pombe that is evolutionarily conserved from amoebae to animals. …”
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1379“…We investigated recombinant human NAT1 and NAT2 expressed in an Escherichia coli JM105 and Schizosaccharomyces pombe expression systems as well as in Chinese hamster ovary (CHO) cells to assess the relative affinity of AcCoA for human NAT1 and NAT2. …”
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1380por Liu, Su-Ling, Narvaez-Ortiz, Heidy Y., Miner, Matt, Kiemel, Jack, Oberhelman, Nicholas, Watt, April, Wagner, Andrew R., Luan, Qing, Helgeson, Luke A., Nolen, Brad J.“…Here, we used structure-based point mutations of the Schizosaccharomyces pombe WDS protein Dip1 to test the importance of its Arp2/3-activating activity in cells. …”
Publicado 2022
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