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“…The observations of the floral biology and breeding system indicated the cross pollination behaviour, which limited the production of selfed seeds and would help to maintain the sustainable levels of heterozygosity among the various populations. …”
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“…After two backcross and three selfing generations, six near isogenic lines (NILs) with 93% recovery of the recurrent parent genome were created from one cross. …”
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“…A sensitive bilobed stigma is thought to assure reproduction, avoid selfing and promote outcrossing. In addition, it may also play a role in pollinator selection since only pollinators with the appropriate body size can trigger this mechanism. …”
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“…The progenies of these treated plants contain up to 75 new ONSEN insertions in their genome which are stably inherited over three generations of selfing. Repeated application of heat stress in progeny plants containing increased numbers of ONSEN copies does not result in increased activation of this transposon compared to control lines. …”
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“…Significant isolation by distance was detected, indicating that the effect of long‐distance gene flow is limited relative to that of genetic drift, probably due to high selfing rates (F(IS) = 0.155–0.887), restricted pollen flow, and ineffective seed dispersal. …”
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“…Moreover, no obvious reduction in GUS levels was observed after three generations of selfing of transgenic plants, indicating that TCSn::GUS is not subject to transgene silencing. …”
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“…At the final stage of random mating, 1000 random fertile plants in the population were identified and subjected to six generations of selfing to produce 1000 immortal MAGIC inbred lines. …”
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“…An alternative is provided by “natural evolution experiments” that started from colonizations or invasions of new habitats by selfing lineages. With limited or missing gene flow from other lineages, new mutations and their effects can be easily detected. …”
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“…One allele, designated Suppressor of HAC1(Cala2) (S-HAC1(Cala2)) encodes a protein with a signal peptide and a dKEE motif with no RxLR motif; the other allele (s-hac1 (Cala2)) encodes a protein with a signal peptide, a dEEE motif and is divergent in sequence from the S-HAC1(Cala2) allele. In selfed progeny from Hpa-Cala2, dominant S-HAC1(Cala2) allele carrying progeny correlates with virulence in RMX-A02, whereas homozygous recessive s-hac1(Cala2) carrying progeny were avirulent. …”
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“…Genetic diversity and SGS were similar among the populations, but offspring showed evidence of inbreeding, mainly originating from mating among related trees, which suggests inbreeding depression between the seed and adult stages. Selfing resulted in a higher inbreeding depression than mating among relatives, as assessed through survival and height. …”
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“…Phytophthora ramorum, P. lateralis and P. crassamura are either selfing, homothallic species, or are known to reproduce exclusively clonally, indicating tolerance to phosphite can emerge even in the absence of sexual recombination.…”
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“…We phased the two subgenomes, one descended from the outcrossing and highly diverse Capsella grandiflora (Cbp(Cg)) and the other one from the selfing and genetically depauperate Capsella orientalis (Cbp(Co)). …”
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“…Contrary to expectations, the facultative xenogamy (mixed mating) species O. benthamii has a relatively low genetic diversity within populations (H = 0.1014, I = 0.1528) and high genetic differentiation among populations (G (ST) = 0.5867, Ф(ST) = 0.659), as is typically found for selfing species. Genetic variance between the two varieties in sympatric populations (44%, Ф(ST) = 0.444) is significantly more than that in allopatric populations (14%, Ф(ST) = 0.138). …”
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“…We tested these hypotheses in a pair of morning glory species that exhibit asymmetric gene flow from highly selfing Ipomoea lacunosa into mixed‐mating Ipomoea cordatotriloba in regions where they co‐occur. …”
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“…RESULTS: G73N covaried with multiple phenological, morphological and ecological traits that improve reproductive fitness: (i) 16–60% higher reproductive allocation; (ii) longer reproduction phase through early flowering (2–7 days); (iii) smaller stigma‐anthers separation (up to 60% reduction of herkogamy and dichogamy) that can potentially promote selfing and reproductive assurance; (iv) ‘winged’ seeds with 30–70% longer sepals that facilitate long‐distance seed dispersal. …”
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