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  1. 2001
    “…Via RNA sequencing, RNA pulldown assay, mass spectrometry, Seahorse, Lipidomics, spheroid formation and cell viability assays, we found that LINC00924 promoted fatty acid (FA) oxidation (FAO) and FA uptake, which was essential for matrix-detached GC cell survival and spheroid formation. …”
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    LINC00924-induced fatty acid metabolic reprogramming facilitates gastric cancer peritoneal metastasis via hnRNPC-regulated alternative splicing of Mnk2
  2. 2002
    “…Moreover, this derivative inhibited cell growth in spheroid cultures in a dose-dependent manner and induced apoptosis in cancer cells. …”
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    Study of the anticancer effect of new quinazolinone hydrazine derivatives as receptor tyrosine kinase inhibitors
  3. 2003
    “…Using both transwell and spheroid co-cultures of LC cells and HSCs, miR-126-3p within EV was established as a mediator of HSC to LC cell communication that influenced tumor cell migration and invasion, as well as the growth of multicellular LC/HSC spheroids. …”
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    Extracellular vesicle-mediated miR-126-3p transfer contributes to inter-cellular communication in the liver tumor microenvironment
  4. 2004
    “…The proposed strategy shows its potential in in vitro cell spheroid production with high cell viability. [Image: see text]…”
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    Miniature Bioreactors: On‐Demand Coalescence and Splitting of Liquid Marbles and Their Bioapplications (Adv. Sci. 10/2019)
  5. 2005
    “…Similar to the property of dandelion flowers dispersing their seeds over a long distance, the DFMs demonstrated enhanced multicellular tumor spheroid (MTS) penetration, a useful property in the treatment of many diseases including cancer, infection-of-biofilm diseases and ocular problems.…”
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    Dandelion flower-like micelles
  6. 2006
    “…Here, VitD already affected the 3D-tumor-spheroid formation, which was not seen in the 2D-cultures. …”
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    Exploiting Vitamin D Receptor and Its Ligands to Target Squamous Cell Carcinomas of the Head and Neck
  7. 2007
    “…In the auxiliary diagnosis, the recall rate of the WI-U-Net++ algorithm for spheroid nodules and irregular nodules was 93.47% and 84.52%, respectively. …”
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    Construction of U-Net++ pulmonary nodule intelligent analysis model based on feature weighted aggregation
  8. 2008
    “…Transcriptomic analysis on spheroids identified decreased apoptosis signaling due to p53(R175H). …”
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    Gain-of-function p53(R175H) blocks apoptosis in a precursor model of ovarian high-grade serous carcinoma
  9. 2009
    “…Cytotoxicity was assessed by IncuCyte live cell imaging, CellTiter-Glo, and spheroid assays. Cell-cycle profiles were determined using propidium iodide staining and flow cytometry. …”
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    A Bifunctional PARP-HDAC Inhibitor with Activity in Ewing Sarcoma
  10. 2010
    por Ng, C. E., Keng, P. C., Sutherland, R. M.
    Publicado 1987
    “…Irradiation of the Q-cells after isolation from spheroids with between 0 to 10 Gy showed that they were more radiosensitive (decreased D0) than the P-cells isolated from these spheroids. …”
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    Characterization of radiation sensitivity of human squamous carcinoma A431 cells.
  11. 2011
    “…CONCLUSIONS: Cytogenetically abnormal spheroid forming, tumorigenic, and invasive neoplastic epithelial cells pre-exist in human DCIS and require cellular autophagy for survival.…”
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    Malignant Precursor Cells Pre-Exist in Human Breast DCIS and Require Autophagy for Survival
  12. 2012
    “…HCO(3)(−) flux inhibition also decelerated HCT116 spheroid growth. In the absence of CO(2)/HCO(3)(−), acid extrusion by H(+) flux in HCT116 and MDA-MB-468 spheroids became highly non-uniform and inadequate at the core. …”
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    Dual Role of CO(2)/HCO(3)(−) Buffer in the Regulation of Intracellular pH of Three-dimensional Tumor Growths
  13. 2013
    “…METHODS: We used in vitro angiogenesis assays: migration, proliferation, differentiation into tube-like structures in Matrigel™ and spheroid development assays in human brain microvessel endothelial cells (hCMEC/D3). …”
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    Citicoline induces angiogenesis improving survival of vascular/human brain microvessel endothelial cells through pathways involving ERK1/2 and insulin receptor substrate-1
  14. 2014
    “…EGFR was inhibited by using the specific small molecule inhibitor AG1478. A specific spheroid growth pattern was observed for all investigated CRC cell lines. …”
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    Impact of the 3D Microenvironment on Phenotype, Gene Expression, and EGFR Inhibition of Colorectal Cancer Cell Lines
  15. 2015
    “…To investigate the role of the CXCL12/CXCR4 axis in maintaining rat GSC properties, we used a spheroid formation assay to assess the stem cell self-renewal properties. …”
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    Disrupting the CXCL12/CXCR4 axis disturbs the characteristics of glioblastoma stem-like cells of rat RG2 glioblastoma
  16. 2016
    “…The viability of tumour spheroids was confirmed after five and ten days by using Annexin V/Propidium Iodide staining for flow-cytometry. …”
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    Development of an Innovative 3D Cell Culture System to Study Tumour - Stroma Interactions in Non-Small Cell Lung Cancer Cells
  17. 2017
    “…The effect of hsa-miR-574-5p on colony formation, cell invasion and cell spheroid formation was investigated by antisense transfected HCT116 cells and miRNA mimic transfected SW1116 cells. …”
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    Hsa-miR-574-5p negatively regulates MACC-1 expression to suppress colorectal cancer liver metastasis
  18. 2018
    “…In contrast, BMD cells formed a spheroid with a smoother and more circular surface when co-cultured with osteoblasts. …”
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    Reduction in Migratory Phenotype in a Metastasized Breast Cancer Cell Line via Downregulation of S100A4 and GRM3
  19. 2019
    “…Taken together, this study represents the most comprehensive evaluation of 3D spheroid hepatic cultures up to now and supports their utility for hepatotoxicity risk assessment in drug discovery. …”
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    Utility of spherical human liver microtissues for prediction of clinical drug-induced liver injury
  20. 2020
    “…MiR655 expression positively correlated with COX-2 in genetically disparate breast cancer cell lines and increased in all cell lines when grown as spheroids, implicating its link with stem-like cells (SLCs). …”
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    COX-2 induces oncogenic micro RNA miR655 in human breast cancer
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