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  1. 2161
    “…The regulation of chemotherapeutic response and necroptotic cell death was assessed using lentiviral YARS overexpression and depletion 3D spheroid formation assay, viability assays, LDH release assay, flow cytometry analysis, and transmission electron microscopy. …”
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    Combined the SMAC mimetic and BCL2 inhibitor sensitizes neoadjuvant chemotherapy by targeting necrosome complexes in tyrosine aminoacyl-tRNA synthase-positive breast cancer
  2. 2162
    por Li, Chunfeng, Qiu, Junqiang, Xue, Yingwei
    Publicado 2021
    “…Annexin V-FITC/PI double staining assay was used to examine cell apoptosis. The Spheroid formation assay was used to evaluated cell stemness. …”
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    Low-dose Diosbulbin-B (DB) activates tumor-intrinsic PD-L1/NLRP3 signaling pathway mediated pyroptotic cell death to increase cisplatin-sensitivity in gastric cancer (GC)
  3. 2163
    “…Clonogenic assay was done to explore the colony formation ability of cells. Spheroid assay was performed to analyze the anti-proliferative effect of NCX4040. …”
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    Exploring NCX4040, an Aspirin Derivative, as a Potential Treatment for Benign Prostatic Hyperplasia
  4. 2164
    “…While all the compounds tested had similar effects on spheroid formation by A431 and SCC13 cells, those with a C1α(OH) group were more potent in inhibiting colony and spheroid formation in the BCC line. …”
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    CYP11A1-derived vitamin D hydroxyderivatives as candidates for therapy of basal and squamous cell carcinomas
  5. 2165
    “…FA-AQ NPs had better anti-tumor abilities demonstrated via 3D spheroid cell culture studies. Therefore, FA-AQ NPs could be a promising drug delivery system for cancer therapy.…”
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    Development and Characterization of Folic Acid-Conjugated Amodiaquine-Loaded Nanoparticles–Efficacy in Cancer Treatment
  6. 2166
    “…Expression of a GLTP mutant incapable of binding VAP disrupted cell spheroid formation, indicating that the GLTP–VAP interaction is linked to cellular adhesion, cohesion, and three-dimensional growth. …”
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    Glycolipid transfer protein knockout disrupts vesicle trafficking to the plasma membrane
  7. 2167
    “…[Image: see text] 3D cell assemblies such as spheroids reproduce the in vivo state more accurately than traditional 2D cell monolayers and are emerging as tools to reduce or replace animal testing. …”
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    Chemically Induced Extracellular Ice Nucleation Reduces Intracellular Ice Formation Enabling 2D and 3D Cellular Cryopreservation
  8. 2168
    “…In this study, we examined the effects of FF along with factors present in the FF on a variety of FTEC models, including primary cell culture, ALI (air–liquid interface) culture, and 3D organ spheroid culture. We found that FF plays a similar role to estrogen in promoting cell differentiation and organoid formation. …”
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    Effects of Follicular Fluid on Physiological Characteristics and Differentiation of Fallopian Tube Epithelial Cells Implicating for Ovarian Cancer Pathogenesis
  9. 2169
    “…In this review, we discuss the current spheroid culture and organ-on-a-chip techniques as well as the main conceptual and technical considerations for the correct establishment of such models. …”
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    The Growing Importance of Three-Dimensional Models and Microphysiological Systems in the Assessment of Mycotoxin Toxicity
  10. 2170
    “…With in vitro neural spheroid cultures, BCCIP deficiency impaired neural progenitor's self-renewal capability, and spontaneously activated p53. …”
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    Requirement of Mouse BCCIP for Neural Development and Progenitor Proliferation
  11. 2171
    “…Emerging evidence indicates that compact spheroid formation is preferentially accomplished by cancer cells with high invasive capacity and contractile behaviors. …”
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    Cell–cell and cell–matrix dynamics in intraperitoneal cancer metastasis
  12. 2172
    “…In addition to the novel ligands, we found that transfection of MRGPRD leads fibroblast cells to form spheroids, which would be related to oncogenicity. …”
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    Identification of Physiologically Active Substances as Novel Ligands for MRGPRD
  13. 2173
    “…RESULTS: Primary cultures of RPE cells were established and spheroid colonies related to progenitor/stem cells developed in a number of cultures. …”
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    Retinal Pigment Epithelium Culture;a Potential Source of Retinal Stem Cells
  14. 2174
    “…It was observed that the Huaier extract significantly inhibited the spheroid formation potential (P<0.05) and decreased the aldehyde dehydrogenase (ALDH)-positive cell population in colorectal primary cancer cells (P<0.05). …”
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    Huaier aqueous extract inhibits colorectal cancer stem cell growth partially via downregulation of the Wnt/β-catenin pathway
  15. 2175
    “…Previously, we enriched a subpopulation of HNSCC-derived spheroid cells (SC) (HNSCC-SC) and identified Nanog as a CSCs marker. …”
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    Enhanced Chemosensitivity by Targeting Nanog in Head and Neck Squamous Cell Carcinomas
  16. 2176
    “…To investigate the role of CCL2 in osteosarcoma cells, MTT, spheroid forming, wound healing and transwell assays were performed to examine the proliferation and invasion abilities of the osteosarcoma cells. …”
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    Monocyte chemotactic protein-1 promotes the proliferation and invasion of osteosarcoma cells and upregulates the expression of AKT
  17. 2177
    por Choudhry, Priya
    Publicado 2016
    “…Due to its subjective and time-intensive nature, manual counting has hindered the adoption of cellular assays such as tumor spheroid formation in high-throughput screens. The objective of this study was to develop an automated method for quick and reliable counting of cells and colonies from digital images. …”
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    High-Throughput Method for Automated Colony and Cell Counting by Digital Image Analysis Based on Edge Detection
  18. 2178
    “…Addition of glucose or lactate to CD133 (−) cells promotes CSC phenotypes, as evidenced by increased CD133 (+) cell population, elevated stemness gene expression and enhanced spheroid formation. Furthermore, the liver-specific miRNA, miR-122, inhibits CSC phenotypes by regulating glycolysis through targeting PDK4. …”
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    Active glycolytic metabolism in CD133(+) hepatocellular cancer stem cells: regulation by MIR-122
  19. 2179
    “…These animals also formed ectopic spheroid structures of neural identity in regenerating heads. …”
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    Integrins are required for tissue organization and restriction of neurogenesis in regenerating planarians
  20. 2180
    por Holmes, Dwayne B., Heine, Vivi M.
    Publicado 2017
    “…After 35 days, differentiation products exhibited similar structures and neuronal markers reported in prior ‘organoid’ and ‘spheroid’ protocols. This included cells positive for KIRREL2 (a marker of early cerebellar neuroepithelium) and ZIC1 (a marker for granule cells). …”
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    Simplified 3D protocol capable of generating early cortical neuroepithelium
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