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2621por Schwefel, Konrad, Spiegler, Stefanie, Ameling, Sabine, Much, Christiane D., Pilz, Robin A., Otto, Oliver, Völker, Uwe, Felbor, Ute, Rath, Matthias“…Induction of apoptosis, sprouting, migration, network and spheroid formation were significantly impaired upon prolonged CCM3 deficiency. …”
Publicado 2018
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2622Cathepsin B defines leader cells during the collective invasion of salivary adenoid cystic carcinomapor Wu, Jia-Shun, Li, Zhu-Feng, Wang, Hao-Fan, Yu, Xiang-Hua, Pang, Xin, Wu, Jing-Biao, Wang, Sha-Sha, Zhang, Mei, Yang, Xiao, Cao, Ming-Xin, Tang, Ya-Jie, Liang, Xin-Hua, Zheng, Min, Tang, Ya-Ling“…CTSB was found to be overexpressed in the invasive front of SACC compared to the tumor center, and was associated with a poor prognosis of patients with SACC. Subsequently, a 3D spheroid invasion assay was established in order to recapitulate the collective cell invasion of SACC and the results revealed that CTSB was only expressed in leader cells. …”
Publicado 2019
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2623“…Additionally, BMP7 silencing and endoglin suppression of Ishikawa cells led to impaired JAr spheroid attachment. These findings suggest that BMP7 is associated with receptivity of the endometrium, indicating that BMP7 regulates receptivity of endometrial epithelial cells for implantation of blastocysts via the endoglin pathway.…”
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2624por Wu, Haojun, He, Yanxia, Chen, Hang, Liu, Yanzhi, Wei, Bo, Chen, Guanghua, Lin, Han, Lin, Hao“…We report that the level of THOR is remarkably upregulated in OS cell spheroids compared to that in OS adherent cells. THOR overexpression increased spheroid formation ability and aldehyde dehydrogenase 1 (ALDH1) activity in OS adherent cells, and the opposite effect was observed in spheroids with THOR knockdown. …”
Publicado 2019
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2625por Xia, Changhong, Zhang, Dongsheng, Li, Yanmei, Chen, Jie, Zhou, Haibo, Nie, Long, Sun, Yanyan, Guo, Siyan, Cao, Jianbiao, Zhou, Fangzheng, Li, Junlai“…Cell migration rate was measured using the xCELLigence system, and the cell invasion ability was examined by the three-dimensional spheroid BME cell invasion assay. The expression level of ADAM17 was also measured with RT-PCR. …”
Publicado 2019
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2626por Du, Kang, Zhao, Can, Wang, Li, Wang, Yue, Zhang, Kang-Zhen, Shen, Xi-Yu, Sun, Hui-Xian, Gao, Wei, Lu, Xiang“…Over-expression of miR-191 promoted apoptosis, but reduced the proliferation, migration, tube-forming and spheroid sprouting activity in HUVECs OGD/R model. …”
Publicado 2019
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2627por Bechmann, Nicole, Poser, Isabel, Seifert, Verena, Greunke, Christian, Ullrich, Martin, Qin, Nan, Walch, Axel, Peitzsch, Mirko, Robledo, Mercedes, Pacak, Karel, Pietzsch, Jens, Richter, Susan, Eisenhofer, Graeme“…Cultivation under extrinsic hypoxia or in spheroid culture (intrinsic hypoxia) increased cellular dopamine and norepinephrine contents in all cell lines. …”
Publicado 2019
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2628por Wiseman, Sam M, Kojic, Liliana D, Kassian, Katayoon, Jones, Steven J, Joshi, Bharat, Nabi, Ivan R“…Gp78/AMFR expression and AMF internalization were measured in differentiated thyroid cancer (DTC) and anaplastic thyroid cancer (ATC) cell lines and in freshly resected human papillary thyroid cancers (PTC) relative to benign thyroid tissue. Spheroid-like aggregates generated from explants of cancer, goiter, and collateral thyroid tissue were assessed for expression of cancer stem cell markers, surface Gp78/AMFR and AMF endocytosis. …”
Publicado 2019
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2629por Becker, Annegret, Lehrich, Tina, Kalies, Stefan, Heisterkamp, Alexander, Ngezahayo, Anaclet“…In 3D matrigel culture system, the GNOME-LP/C-CPE-AuNP completely destroyed spheroids composed of Caco-2 cells and reduced OE-33 cell spheroid formation. …”
Publicado 2019
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2630por Sexton, Rachel, Mahdi, Zaid, Chaudhury, Rahman, Beydoun, Rafic, Aboukameel, Amro, Khan, Husain Y., Baloglu, Erkan, Senapedis, William, Landesman, Yosef, Tesfaye, Anteneh, Kim, Steve, Philip, Philip A., Azmi, Asfar S.“…SINE compounds inhibited gastric cancer cell proliferation, disrupted spheroid formation, induced apoptosis and halted cell cycle progression at the G1/S phase. …”
Publicado 2019
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2631por Huang, Chung-Yen, Huang, Ching-Ying, Pai, Yu-Chen, Lin, Been-Ren, Lee, Tsung-Chun, Liang, Pi-Hui, Yu, Linda Chia-Hui“…Lastly, 5-FU-induced tumor spheroid destruction was prevented by glucose and pyruvate, but not by ATP. …”
Publicado 2019
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2632por Benzing, Christian, Lam, Hoyin, Tsang, Chi Man, Rimmer, Alexander, Arroyo-Berdugo, Yoana, Calle, Yolanda, Wells, Claire M.“…METHODS: To specifically test the impact of interaction on invasive potential two PDAC cell lines PaTu8902 and CFPAC-1 were selected on their ability to form invasive adhesions, otherwise known as invadopodia and invade in a spheroid invasion assay. RESULTS: Interestingly when the PDAC cells were co-cultured with undifferentiated THP1 monocyte-like cells invadopodia formation was significantly suppressed. …”
Publicado 2019
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2633por VELIER, Mélanie, SIMONCINI, Stéphanie, ABELLAN, Maxime, FRANCOIS, Pauline, EAP, Sandy, LAGRANGE, Anaïs, BERTRAND, Baptiste, DAUMAS, Aurélie, GRANEL, Brigitte, DELORME, Bruno, DIGNAT GEORGE, Françoise, MAGALON, Jérémy, SABATIER, Florence“…The angiogenic activity of endothelial cells was measured in vitro using tube formation and spheroid assays. DF collagen and alpha smooth muscle actin (αSMA) content were quantified after five days of co-culture with ASC. …”
Publicado 2019
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2634por Öztürk Akcora, Büsra, Vassilios Gabriël, Alexandros, Ortiz‐Perez, Ana, Bansal, Ruchi“…Finally, in LPS‐induced human hepatic 3D spheroid model, WP1066 inhibited LPS‐induced fibrotic and inflammatory parameters. …”
Publicado 2020
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2635por Salcher, Stefan, Spoden, Gilles, Huber, Julia M., Golderer, Georg, Lindner, Herbert, Ausserlechner, Michael J., Kiechl-Kohlendorfer, Ursula, Geiger, Kathrin, Obexer, Petra“…Importantly, FOXO3 inhibition by RPG represses the binding of FOXO3 to the LUM promoter, inhibits FOXO3-mediated LUM RNA and protein expression, and efficiently abrogates FOXO3-triggered cellular “wound healing” as well as spheroid-based 3D-migration. Thus, silencing the FOXO3/LUM axis by the FDA-approved compound RPG represents a promising strategy for novel therapeutic interventions in NB and other FOXO3-dependent tumors.…”
Publicado 2019
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2636por Kuritz, Karsten, Stöhr, Daniela, Maichl, Daniela Simone, Pollak, Nadine, Rehm, Markus, Allgöwer, Frank“…As use cases, we applied MAPiT to two prominent problems in the flow-cytometric analysis of heterogeneous cell populations: (1) recovering the kinetics of cell cycle progression in unsynchronised and thus unperturbed cell populations, and (2) recovering the spatial arrangement of cells within multi-cellular spheroids prior to spheroid dissociation for cytometric analysis. …”
Publicado 2020
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2637por Murgoci, Adriana-Natalia, Duhamel, Marie, Raffo-Romero, Antonella, Mallah, Khalil, Aboulouard, Soulaimane, Lefebvre, Christophe, Kobeissy, Firas, Fournier, Isabelle, Zilkova, Monika, Maderova, Denisa, Cizek, Milan, Cizkova, Dasa, Salzet, Michel“…These results were confirmed using the neurite outgrowth assay on DRGs cells and glioma proliferation analysis in 3D spheroid cultures. Results from these in vitro assays suggest that the microglia localized at different CNS regions can ensure different biological functions. …”
Publicado 2020
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2638por Borgna, Vincenzo, Lobos-González, Lorena, Guevara, Francisca, Landerer, Eduardo, Bendek, Maximiliano, Ávila, Rodolfo, Silva, Verónica, Villota, Claudio, Araya, Mariela, Rivas, Alexis, López, Constanza, Socias, Teresa, Castillo, Jorge, Alarcón, Luis, Burzio, Luis O., Burzio, Verónica A., Villegas, Jaime“…Treatment also inhibited cell invasion and spheroid formation together with inhibition of N-cadherin and MMP 11. …”
Publicado 2020
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2639“…Likewise, there are also scaffold free techniques used such as hanging drop microplates, magnetic levitation, and spheroid microplates with ultra-low attachment coating. 3D cell culture has the potential to provide alternative ways to study organ behavior via the use of organoids and is expected to eventually bridge the gap between 2D cell culture and animal models. …”
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2640por Chen, Hong, Fu, Huancheng, Wu, Xue, Duan, Yufeng, Zhang, Sicheng, Hu, Hong, Liao, Yuansong, Wang, Tao, Yang, Yan, Chen, Guoqing, Li, Zhonghan, Tian, Weidong“…In this study, through three-dimensional spheroid culture, a group of unique multipotent stem cells were identified from mouse dental papilla called multipotent dental pulp regenerative stem cells (MDPSCs). …”
Publicado 2020
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