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  1. 2681
    “…For this reason, new models that more closely resemble the in vivo microenvironment, such as three-dimensional cell co-cultures or organotypic multicellular spheroid models, need to be developed and investigated.…”
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  2. 2682
    “…Furthermore, three-dimensional spheroid cultures of HepG2/8F_HS cells showed a further increase in liver functions upon heat treatment. …”
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  3. 2683
    “…Next, sorted ECs were examined for their ability to act as typical vascular ECs through capillary-like structure formation assay, Dil-acetylated low-density lipoprotein (Dil-Ac-LDL) uptake, and three-dimensional spheroid sprouting. Consequently, pEpiSCs-derived ECs function as typical vascular ECs, indicating that pEpiSC-derived ECs might be used to develop cell therapeutics for vascular disease.…”
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  4. 2684
    “…However, compared to BPH-1 cells, the 22Rv1 cancer cells showed increased cytopathic effect, higher induction of caspase-8 and -9, and extensive syncytia formation. In 22Rv1 spheroid cultures, P/V/F infection was less efficient compared to monolayers, but the virus was able to spread through spheroids and induce death. …”
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  5. 2685
    “…Over 90% DANCR silencing was observed along with inhibition of cell migration, invasion, and spheroid formation relative to transfection with negative control siRNA in RGD-PEG-ECO nanoparticles. …”
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  6. 2686
    “…GP-2250 showed an antiadhesive effect on JL-1 and MSTO-211H spheroids. It had a dose-dependent cytotoxic effect on both monolayer and spheroid cultured cells, inducing apoptosis and necrosis. …”
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  7. 2687
    “…KLK6 suppression in HCT-116 colon cancer cells decreased the colony formation, increased cell adhesion to extracellular matrix proteins, and reduced spheroid formation and compaction. Immunohistochemistry (IHC) analysis demonstrated ectopic expression of KLK6 in human colon adenocarcinomas but not in normal epithelia. …”
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  8. 2688
  9. 2689
    “…Results: We observed that the cells cultured as spheroids had a slightly lower viability and a reduced proliferation rate but a higher expression of the stemness-related transcriptional factors compared to the cells cultured in monolayer. …”
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  10. 2690
    “…Immunohistochemical analysis of adhesion tissues revealed a loosened adhesive band between tissues, coupled with significantly decreased peritoneal thickening in the lumbrokinase-treated group versus the control group. Three-dimensional spheroid, MTT, and scratch wound migration assays using the IMR-90 human fibroblast cell line demonstrated that lumbrokinase significantly attenuated the migration and adhesive activity of fibroblasts without compromising cell proliferation. …”
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  11. 2691
    “…Despite that, long-term treated cells exhibited diminished spheroid growth and significantly reduced migration capacity per se and to differentiated osteoclasts. …”
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  12. 2692
    “…In addition to its anti-apoptotic and bioenergetic functions, MNRR1 is both necessary and sufficient for a focal adhesion and ECM repertoire that can support spheroid formation. Its ectopic expression is sufficient to induce the adhesive glycoprotein THBS4 and the type 1 collagen, COL1A1. …”
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  13. 2693
    “…The results showed that BPFL and BPC (formed by an additional ring system) were the most cytotoxic analogues that affected cell viability, spheroid surface area and morphology, cell proliferation, and apoptotic cell death. …”
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  14. 2694
    “…Here, we evaluated TIMP1 protein and mRNA levels in bone marrow (BM) plasma cells and assessed the effects of TIMP1 expression on fibroblast invasive capacity using three-dimensional spheroid cell invasion assays. TIMP1 mRNA and protein levels were elevated when patients progressed from monoclonal gammopathy of undetermined significance or smouldering myeloma to MM. …”
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  15. 2695
  16. 2696
    “…To this end, we treated CCSCs with different concentrations of VD and performed spheroid formation assay and transmission electron microscopy and determined cysteine (Cys), glutathione (GSH), and reactive oxygen species (ROS) levels. …”
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  17. 2697
    por Krauss, George, 1933-
    Publicado 2015
    Tabla de Contenidos: “…Introduction: Purpose of text, microstructure and analysis, steel definitions, and specifications -- History and primary steel processing -- Phases and structures -- Pearlite, ferrite, and cementite -- Martensite -- Bainite -- Ferritic microstructures -- Austenite in steel -- Primary processing effects on steel microstructure and properties -- Isothermal and continuous cooling transformation diagrams -- Deformation, strengthening, and fracture of ferritic microstructures -- Low-carbon steels -- Normalizing, annealing, and spheroidizing treatments; ferrite/pearlite and spherical carbides -- Non-martensitic strengthening of medium-carbon steels-microalloying and bainitic strengthening -- High-carbon steels-fully pearlitic microstructures and wire and rail applications -- Hardness and hardenability -- Tempering of steel -- Deformation, mechanical properties, and fracture of quenched and tempered carbon steels -- Low toughness and embrittlement phenomena in steels -- Residual stresses, distortion, and heat treatment -- Surface hardening -- Surface modification -- Stainless steels -- Tool steels -- Appendix: Hardness conversions.…”
    Libro
  18. 2698
    “…Lf‐LP‐Mu‐DTX increased uptake in both U87‐MG cells and hCMEC/D3 cells, enhanced penetration of U87‐MG tumor spheroid and in vitro BBB model, had better in vitro and in vivo anti‐tumor effects. …”
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  19. 2699
    “…The findings were finally validated in breast and lung spheroid models composed of differentiated cancer cells and cancer stem cells at the ratio of 1:1 and embedded in Matrigel dopped with negative‐, neutral‐ and positive‐charged polymers and CSCs prefer to spread out of spheroids to lower electrostatic potential sites. …”
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  20. 2700
    “…Wound healing and Transwell assays were used to measure cell migration. Spheroid formation assay were used to assess stem-like properties. …”
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