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2701por Vallino, Letizia, Garavaglia, Beatrice, Visciglia, Annalisa, Amoruso, Angela, Pane, Marco, Ferraresi, Alessandra, Isidoro, Ciro“…RESULTS AND CONCLUSION: Probiotic metabolites reduced cell proliferation both in 2D and 3D-spheroid cultures, the latter model mimicking the growth in vivo. …”
Publicado 2023
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2702por El-Arabey, Amr Ahmed, Alkhalil, Samia S., Al-Shouli, Samia T., Awadalla, Maaweya E., Alhamdi, Heba W., Almanaa, Taghreed N., Mohamed, Samah Saif Eldin M., Abdalla, Mohnad“…TAMs play a crucial role in the metastasis of OC in the peritoneum and ascities by assisting in spheroid formation and cancer cell adhesion to the metastatic regions. …”
Publicado 2023
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2703por Mabate, Blessing, Daub, Chantal Désirée, Pletschke, Brett Ivan, Edkins, Adrienne Lesley“…The potency of fucoidans on the 2D and 3D migration of HCT116 cells was investigated by wound healing assay and spheroid migration assays, respectively. Lastly, the anti-cell adhesion potential of fucoidans on HCT116 cells was also investigated. …”
Publicado 2023
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2704por El-Hattab, Mariam Y., Sinclair, Noah, Liszewski, Jesse N., Schrodt, Michael V., Herrmann, Jacob, Klingelhutz, Aloysius J., Sander, Edward A., Ankrum, James A.“…To better understand this role, we developed a three-dimensional human adipocyte spheroid system that has an adipokine profile similar to in vivo adipose tissues. …”
Publicado 2023
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2705“…Cell viability, glycolytic activity, Annexin V-PE binding activity, reactive oxygen species (ROS) levels, mitochondrial membrane potential, ATP content, western blot analysis and spheroid viability were assessed in the present study. …”
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2706por Coskun, Goknil Pelin, Ozhan, Yagmur, Dobričić, Vladimir, Bošković, Jelena, Reis, Rengin, Sipahi, Hande, Sahin, Zafer, Demirayak, Seref“…Compounds 7a, 7d, and 7f showed the best 3D cell line inhibitory effect according to spheroid diameters. The compounds were screened for their COX-2 and 5-LOX inhibitory activity. …”
Publicado 2023
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2707por Wang, Yang, Wang, Zheng, Wu, Xiao, Zhu, Song, Guo, Qiru, Jin, Zhong, Chen, Zixian, Zhang, Delai, Hu, Wangming, Xu, Huan, Shi, Liangqin, Yang, Lan, Wang, Yong“…BRDU Incorporation Assay, Boyden Chamber Migration Assay, Tube Formation Assay, and Spheroid Sprouting Assay were used to investigate whether Paeonol is involved in regulating proliferation and migration of endothelial cells. …”
Publicado 2023
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2708por Nashimoto, Yuji, Shishido, Shotaro, Onuma, Kunishige, Ino, Kosuke, Inoue, Masahiro, Shiku, Hitoshi“…As models of tumours with heterogeneous subpopulations, we used patient-derived cancer organoids with a distinct growth potential established using the cancer tissue-originated spheroid methodology. Scanning electrochemical microscopy measurements enabled the analysis of the oxygen consumption rate (OCR) for individual organoids as small as 100 µm in diameter and could detect the heterogeneity amongst studied subpopulations, which was not observed in conventional colorectal cancer cell lines. …”
Publicado 2023
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2709por Lee, Yoon Jin, Heo, Jae Young, Kim, Dong Sung, Choi, Yu Sung, Kim, Sooyoung, Nam, Hae Seon, Lee, Sang Han, Cho, Moon Kyun“…METHODS: We used 2D monolayer and 3D spheroid human epidermal melanocyte culture models, HEMn-MP (human epidermal melanocytes, neonatal, moderately pigmented), and two human MM cell lines, G361 and SK-MEL-2, to evaluate cell viability, proliferation, migration, death, and reactive oxygen species (ROS) production following single therapy treatment, with either curcumin or binimetinib, or a combination of both. …”
Publicado 2023
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2710“…Three human glioblastoma multiforme (GBM) cell lines were tested on the scaffolds to reveal the influence of pore size on cell growth kinetics, tumor spheroid morphology, gene expression and dose-dependent drug response. …”
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2711por Mayr, Christian, Kiesslich, Tobias, Bekric, Dino, Beyreis, Marlena, Kittl, Michael, Ablinger, Celina, Neureiter, Elen, Pichler, Martin, Prinz, Felix, Ritter, Markus, Neureiter, Daniel, Jakab, Martin, Dobias, Heidemarie“…Furthermore, using a 3D cell culture model, we found that ouabain disturbs spheroid growth and reduces the viability of biliary tract cancer cells within the tumor spheroids. …”
Publicado 2023
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2712“…RESULTS: Results uncovered that E2F3 was upregulated in spheroid cells. In addition, E2F3 facilitates stemness in colon cancer. …”
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2713por Johnson, Krystal C., Kilikevicius, Audrius, Hofman, Cristina, Hu, Jiaxin, Liu, Yang, Aguilar, Selina, Graswich, Jon, Han, Yi, Wang, Tao, Westcott, Jill M., Brekken, Rolf A., Peng, Lan, Karagkounis, Georgios, Corey, David R.“…We show nuclear enrichment of AGO2 in HCT116 cells grown in two-dimensional culture to high density, HCT116 cells grown in three-dimensional tumor spheroid culture, and human colon tumors. The shift in localization of AGO2 from cytoplasm to nucleus de-represses cytoplasmic AGO2-eCLIP targets that were candidates for canonical regulation by miRISC. …”
Publicado 2023
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2714por Charvátová, Hana, Plichta, Zdeněk, Hromádková, Jiřina, Herynek, Vít, Babič, Michal“…Transmission electron microscopy (TEM) images show a narrow size distribution and spheroid shape. Alternative coatings were prepared by copolymerization of HPMA with methyl 2-(2-methylprop-2-enoylamino)acetate (MMA) and further post-polymerization modification with hydroxamic acid groups, carboxylic acid and primary-amino functionalities. …”
Publicado 2023
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2715por Bucki, Robert, Iwamoto, Daniel V., Shi, Xuechen, Kerr, Katherine E., Byfield, Fitzroy J., Suprewicz, Łukasz, Skłodowski, Karol, Sutaria, Julian, Misiak, Paweł, Wilczewska, Agnieszka Z., Ramachandran, Sekar, Wolfe, Aaron, Thanh, Minh-Tri Ho, Whalen, Eli, Patteson, Alison E., Janmey, Paul A.“…Using traction force microscopy and spheroid expansion assays, we characterize how different cell types respond to extracellular vimentin. …”
Publicado 2023
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2716por Tumas, Sarunas, Meldgaard, Trine Sundebo, Vaaben, Troels Holger, Suarez Hernandez, Sara, Rasmussen, Annemette Tengstedt, Vazquez-Uribe, Ruben, Hadrup, Sine Reker, Sommer, Morten O. A.“…Next, we assessed the immune modulatory potential of mi-IL2 using a 3D tumor spheroid model demonstrating a strong effect on immune cell activation. …”
Publicado 2023
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2717por Louback, Rafaela de Assiz, Martins-Cardoso, Karina, Tinoco, Luzineide W., Collino, Federica, de Barros, Ana Paula D. N., Fortuna-Costa, Anneliese, Monteiro, Robson Q., Rossi, Maria Isabel Doria, Lindoso, Rafael Soares“…Furthermore, invasion assays using a three-dimensional (3D) fibroblast spheroid model showed reduced MDA-MB-231 invasion towards fibroblast spheroids pretreated with EV-ASA as compared to spheroids prepared with EV-CTR-stimulated fibroblasts. …”
Publicado 2023
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2718por Burmatova, Anastasia, Khannanov, Artur, Gerasimov, Alexander, Ignateva, Klara, Khaldeeva, Elena, Gorovaia, Arina, Kiiamov, Airat, Evtugyn, Vladimir, Kutyreva, Marianna“…The cobalt-containing nanocomposite (CoNP) had a high colloidal stability and contained spheroid polymer aggregates with a diameter of 35–50 nm with immobilized cobalt nanoparticles of 5–7 nm. …”
Publicado 2023
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2719por He, Baoyu, Liang, Jing, Qin, Qianqian, Zhang, Yuqin, Shi, Shuo, Cao, Jinghe, Zhang, Zhixin, Bie, Qingli, Zhao, Rou, Wei, Li, Zhang, Baogui, Zhang, Bin“…Here, we report the up-regulation of IL-13RA2 expression in colorectal cancer (CRC) tissues and spheroid cells. The expression of IL-13RA2 was positively correlated with canonical stemness markers in CRC. …”
Publicado 2023
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2720por Żelechowska-Matysiak, Kinga, Salvanou, Evangelia-Alexandra, Bouziotis, Penelope, Budlewski, Tadeusz, Bilewicz, Aleksander, Majkowska-Pilip, Agnieszka“…Dose-dependent additive and synergistic effects of the radiobioconjugate were also shown in spheroid models. Ex vivo biodistribution experiments were performed in SKOV-3 tumor-bearing mice, investigating different distributions of the (198)AuNPs-DOX and DOX-(198)AuNPs-Tmab after intravenous (i.v.) and intratumoral (i.t.) administration. …”
Publicado 2023
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