“…Targeting the mitochondrial pyruvate carrier (MPC) promotes alanine oxidation to mitigate doxSL synthesis and improves spheroid growth, while direct inhibition of doxSL synthesis drives similar phenotypes. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The present paper studies the locations and linear stability of the triangular equilibrium points when both primaries are radiating and considered as heterogeneous spheroid with three layers of different densities. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…In addition to an alteration in the epithelial-to-mesenchymal transition (EMT) marker expression, we observed PD-L1-dependent cell spreading, migration and invasion in a spheroid spreading assay on four different coatings (poly-L-lysine, collagen type I, fibronectin and Matrigel(®)) and a chemotactic transwell migration/invasion assay. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The viability of cell spheroids was analyzed using a Live/Dead kit assay and a Cell Counting Kit-8 assay. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…VDR, RORγ, and RORα were also detected in SKOV-3 and OVCAR-3 cell lines with increased expression in the latter line. 20-Hydroxy-lumisterol3 (20(OH)L(3)) and synthetic RORα/RORγ agonist SR1078 inhibited proliferation only in the OVCAR-3 line, while 20-hydroxyvitamin-D(3) (20(OH)D(3)) only inhibited SKOV-3 cell proliferation. 1,25(OH)(2)D(3), 20(OH)L(3), and SR1078, but not 20(OH)D(3), inhibited spheroid formation in SKOV-3 cells. In summary, decreases in VDR, RORγ, and RORα expression correlated with an unfavorable outcome for OC, and compounds targeting these receptors had a context-dependent anti-tumor activity in vitro. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Overexpression of TUNAR not only impaired spheroid attachment to EECs, but also inhibited decidualization of ESCs. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…In vitro, co-administering iRGD with d-SN38@NPs+laser showed higher cellular uptake, apoptosis ratio and multicellular spheroid penetration. In vivo, d-SN38@NPs/iRGD+laser displayed advanced penetration and accumulation in tumor, leading to 60.89% of tumor suppression in 4T1 tumor-bearing mouse model with a favorable toxicity profile. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…We measure the uptake of the inhibitor AZD2014 (7 μM) in monolayer HEK293 cells occurring with a half-life of 1 min but observe complex behaviour for 3D spheroids with the core of the spheroid showing a slower uptake and a slow biphasic behaviour at longer times. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Herein, we used diclofenac (DIC) repurposed to control MCT activity, and Koningic acid (KA) that is a GAPDH partial inhibitor, and observed that we can control the subpopulation of cancer cells with WE phenotype within a tumor in favor of a less aggressive phenotype without a WE to control progression and metastasis. In a 3D spheroid co-cultures, we showed that our strategy can control the growth of more aggressive MDA-MB-231 cells, while sparing the less aggressive MCF7 cells. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…We extended the characterizations of BI and PI in Wnt-dependent squamous cell carcinoma cell line NCI-H1703 on several CSC functions and found that BI was better at inhibiting soft agar colony formation as an output of self-renewal ability, whereas PI was more effective in inhibiting the growth of multicellular tumor spheroid model mimicking micrometastases. Both however were not able to inhibit migration and invasion of NCI-H1703. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Here, we present computational 4D-OCM methods that enable the study of dynamic invasion behavior of large tumor spheroids embedded in collagen. Our multi-day, time-lapse imaging data provided detailed visualizations of evolving spheroid morphology, collagen degradation, and collagen deformation, all using label-free scattering contrast. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…We identified PEGylated CAIs DTP1K 28, DTP2K 23, and DTP3.4K 29, bearing short and medium PEG backbones, as the most efficient conjugates under both normoxic and hypoxic conditions, and in the tumor spheroid models. PEGylated CAIs did not affect the cell viability of CA IX-negative NCI-H23 tumor spheroids, thus confirming a CA IX-mediated cell killing for these potential anticancer agents.…”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Cancer stem cell phenotypes were assessed by spheroid formation. The proteins involved in cell migration and EMT were evaluated by Western blot analysis and immunofluorescence staining. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…However, the beta‐like cells within the spheroid do not polarize in response to this basement membrane. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The improvement is due to phase purity, stable single-domain (SSD) size, and shape anisotropy originating from the prolate spheroid particles.…”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Curcumin significantly (p < 0.001) suppressed colonies formation by 50% and shrank the spheroids in CSC subpopulations, indicating inhibition of their self-renewal capability. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…We determined that KHDRBS3 might play a crucial role in the acquisition of stem cell properties, such as drug resistance and spheroid/organoid formation, by regulating CD44 variant expression and the Wnt signaling pathway. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Our rabbit intestinal spheroid and monolayer cultures grew most efficiently in L-WRN-conditioned medium that contained Wnt, R-spondin and Noggin, and that had been supplemented with ROCK and TGF-β inhibitors. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…In the cellular inflammation model, murine BV-2 microglial cells changed their morphology from normal spheroid to activated spindle shape after 24 h of induction of LPS. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Artocarpin also dramatically suppressed anchorage-independent growth, cancer stem cell (CSC) spheroid formation, and viability of CSC-rich spheroids. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso