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3281por Cho, Sumin, Choi, Hyemin, Jeong, Hyundoo, Kwon, Su Yeon, Roh, Eun Ji, Jeong, Kwang-Hun, Baek, Inho, Kim, Byoung Ju, Lee, Soo-Hong, Han, Inbo, Cha, Jae Min“…First, we built a large-scale manufacturing platform for MSC spheroids, which is applicable to good manufacturing practice (GMP). …”
Publicado 2022
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3282por Kapeleris, Joanna, Müller Bark, Juliana, Ranjit, Shanon, Richard, Derek, Vela, Ian, O’Byrne, Kenneth, Punyadeera, Chamindie“…RESULTS: Proliferation, spheroid-formation and colony formation ability under varying O(2) levels were investigated. …”
Publicado 2022
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3283The miR-182-5p/NDRG1 Axis Controls Endometrial Receptivity through the NF-κB/ZEB1/E-Cadherin Pathwaypor Yu, Seong-Lan, Kang, Yujin, Jeong, Da-Un, Lee, Dong Chul, Jeon, Hye Jin, Kim, Tae-Hyun, Lee, Sung Ki, Han, Ae Ra, Kang, Jaeku, Park, Seok-Rae“…NDRG1-overexpressing or -depleted cells showed altered attachment rates of JAr spheroids. Collectively, our findings indicate that miR-182-5p-mediated NDRG1 downregulation impaired embryo implantation by upregulating the NF-κΒ/ZEB1/E-cadherin pathway. …”
Publicado 2022
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3284por Liao, Mianmian, Du, Haiyan, Wang, Bing, Huang, Jinzhen, Huang, Danping, Tong, Guangdong“…Several endpoints, including spheroid formation and differentiation, cell proliferation, surface markers of LCSCs, PPI binding targets, and stemness-associated protein expression, were evaluated. …”
Publicado 2022
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3285“…Overexpression of 14-3-3τ induces ERα36 in xenografts and tumor spheroids. The regulation is further supported by a positive correlation between ERα36 and 14-3-3τ expression in human breast cancers. …”
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3286“…We further investigated our 47 patient‐derived colorectal cancer‐stem cell spheroid lines. The 18 responsive lines showed relatively high F/E (median, 16.4), whereas 29 nonresponsive lines had low F/E (median, 9.2) (p = 0.0006). …”
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3287“…The flexibility of Blender is exploited to simulate 5 models with real-life relevance to clinical SFDI of diseased tissue: flat samples containing a single material, flat samples containing 2 materials, flat samples containing 3 materials, flat samples with spheroidal tumours and cylindrical samples with spheroidal tumours. …”
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3288por Song, Soonhwa, Park, Joon Kyu, Shin, Sang Chul, Lee, Jae-Jin, Hong, Seung Kon, Song, In-Kang, Kim, Bokyung, Song, Eun Joo, Lee, Kong-Joo, Kim, Eunice EunKyeong“…In addition, depletion of FAF1 increased collective invasion in a 3D spheroid cell culture. These results provide insight into how the FAF1–Hsp70 complex acts as a novel regulator of the adherens junction integrity. …”
Publicado 2022
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3289por Wysmolek, Paulina M., Kiessler, Filippo D., Salbaum, Katja A., Shelton, Elijah R., Sonntag, Selina M., Serwane, Friedhelm“…As a proof of principle, we created a 3D connectivity map of a stem cell derived neuron spheroid by imaging its activity. Our fast, low complexity setup empowers researchers to study the formation of neuronal networks in vitro for fundamental and neurodegeneration research.…”
Publicado 2022
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3290por Lee, Yun Hee, Choi, Hui-Ji, Kim, Ji Yea, Kim, Ji-Eun, Lee, Jee-Hyun, Cho, So-Hyun, Yun, Mi-Young, An, Sungkwan, Song, Gyu Yong, Bae, Seunghee“…Treatment of the DP spheroids with Rg4 (20 to 50 μg/ml) significantly increased the viability and size of the DP spheres in a dose-dependent manner. …”
Publicado 2021
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3291“…The introduced iRGD keeps tumor-targeting and -penetrating bioactivity, which endows iRGD-NPs with ~100% of tumor-cell uptake and excellent tumor spheroid-penetration, and thus iRGD-NPs can efficiently deliver siVEGF to significantly inhibit angiogenesis in zebrafish and tumor growth in nude mice bearing breast cancer without obvious toxicity. …”
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3292por Deb, Dhruba, Wu, Yangfan, Coker, Courtney, Harimoto, Tetsuhiro, Huang, Ruoqi, Danino, Tal“…We screened 10 engineered bacterial toxins across 6 non-small cell lung cancer patient-derived cell lines and identified theta toxin as a promising therapeutic candidate. Using a bacteria-spheroid co-culture system (BSCC), analysis of differentially expressed transcripts and gene set enrichment revealed significant changes in at least 10 signaling pathways with bacteria-producing theta toxin. …”
Publicado 2022
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3293“…A significant increase in tumor spheroid formation with upregulation of β-catenin and CD133 was also observed. …”
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3294“…The spheroidal green algae Volvox carteri serves as a model system to investigate the formation of a complex, multifunctional extracellular matrix (ECM) in a relatively simple, multicellular organism with cell differentiation. …”
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3295por Weisser, Hannah, Göbel, Tamara, Melissa Krishnathas, G., Kreiß, Marius, Angioni, Carlo, Sürün, Duran, Thomas, Dominique, Schmid, Tobias, Häfner, Ann-Kathrin, Kahnt, Astrid S.“…Furthermore, cell viability, proliferation, migration and multicellular tumor spheroid (MCTS) formation were studied in these cells. …”
Publicado 2022
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3296por Kang, Seong Goo, Choi, Yoon Young, Mo, Sung Jun, Kim, Tae Hyeon, Ha, Jang Ho, Hong, Dong Ki, Lee, Hayera, Park, Soo Dong, Shim, Jae-Jung, Lee, Jung-Lyoul, Chung, Bong Geun“…When the hepG2 spheroids cultured with microbiota-derived metabolites, we observed the changes in the physiological function of hepG2 spheroids, showing that the albumin and urea secretion activity of liver spheroids was significantly enhanced. …”
Publicado 2023
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3297“…The sporophyte appeared in a barrel shape, the average of the parent cell in the isolate, and the spheroid was mediated by the parent cell, and the cells of the isolate were characterized by their small size and the location of the sporophyte under the two ends where the most isolated were Bacillus, Enterobacteriaceae species. …”
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3298por Jia, Yu, Li, Hui-Yan, Wang, Jue, Chen, Xing, Lou, Lu, Wei, Yan-Yan, Wang, Ying, Mo, Shi-Jing“…Methods: Functional studies were determined by immunohistochemistry of PDAC samples from patients and Pdx1-Cre; LSL-Kras(G12D/+); Trp53(fl/+) (KPC) mice, three-dimensional spheroid culture, flow cytometry, MTT and subcutaneous xenograft experiments. …”
Publicado 2023
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3299por Jin, Zhong, Guo, Qiru, Wang, Zheng, Wu, Xiao, Hu, Wangming, Li, Jiali, Li, Hongfei, Zhu, Song, Zhang, Haidi, Chen, Zixian, Xu, Huan, Shi, Liangqin, Yang, Lan, Wang, Yong“…BrdU incorporation assay, Boyden chamber migration assay, spheroid sprouting assay, and Matrigel-based tube formation assay were performed to evaluate whether andrographolide regulates the proliferation and migration of vascular endothelial cells. …”
Publicado 2023
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3300por Phukhum, Pimpawadee, Phetcharaburanin, Jutarop, Chaleekarn, Kwuanjira, Kittirat, Yingpinyapat, Kulthawatsiri, Thanaporn, Namwat, Nisana, Loilome, Watcharin, Khuntikeo, Narong, Titapun, Attapol, Wangwiwatsin, Arporn, Khampitak, Tueanjit, Suksawat, Manida, Klanrit, Poramate“…The three-dimensional multicellular spheroid (3D MCS) model has been employed in cholangiocarcinoma research as it generates 3D architecture and includes more physiological relevance with the multicellular arrangement. …”
Publicado 2023
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