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  1. 4261
    por Clermont, Yves
    Publicado 1956
    “…Electron microscopic study of the Golgi zone of the rat spermatids revealed two main types of vesicular structures: flat vesicles generally accumulated in stacks and spheroidal vesicles of various sizes. The Golgi zone of young spermatids showed a predominance of flat vesicles and small spherical vesicles, while the "residual" Golgi zone of the maturing spermatids displayed an increased number of large spherical vesicles and thus became increasingly vacuolated in appearance. …”
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    THE GOLGI ZONE OF THE RAT SPERMATID AND ITS ROLE IN THE FORMATION OF CYTOPLASMIC VESICLES
  2. 4262
    “…In contrast, the size of 3D spheroids could be measured accurately. Immunostaining of spheroids grown in the 3D culture system showed an increase in the DNA double-strand-break marker γH2AX one hour after irradiation. …”
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    Establishment of a 3D Model to Characterize the Radioresponse of Patient-Derived Glioblastoma Cells
  3. 4263
    “…E-cadherin expression in EMT/6 cells resulted in tighter adhesion of multicellular spheroids and a reduced proliferative fraction in three-dimensional culture. …”
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    E-Cadherin–dependent Growth Suppression is Mediated by the Cyclin-dependent Kinase Inhibitor p27(KIP1)
  4. 4264
    “…One of the characteristics of CSCs is their ability to form floating spheroids under anchorage-independent conditions in a serum-free defined media. …”
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    The Wnt/β-catenin pathway regulates growth and maintenance of colonospheres
  5. 4265
    “…We have studied epithelial tissue architecture using 3D culture models and found that adult primary prostate epithelial cells grow into hollow acinus-like spheroids. Importantly, when co-cultured with stroma the epithelia show increased lateral cell adhesions. …”
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    Stroma Regulates Increased Epithelial Lateral Cell Adhesion in 3D Culture: A Role for Actin/Cadherin Dynamics
  6. 4266
    “…In addition, multicellular tumor spheroids were used to study the involvement of the tumor microenvironment in invasion. …”
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    Hypoxia stimulates migration of breast cancer cells via the PERK/ATF4/LAMP3-arm of the unfolded protein response
  7. 4267
    por Das, Jayanta K., Felty, Quentin
    Publicado 2014
    “…Our results showed that PCB-induced ROS mediated a highly tube branched neovascular phenotype that also depended on ID3 and Pyk2; and PCB153 treatment increased the size of endothelial spheroids under conditions typically used for clonal selection of stem cell spheroids. …”
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    PCB153-Induced Overexpression of ID3 Contributes to the Development of Microvascular Lesions
  8. 4268
    “…By increasing cell-cell cohesion, Dex also decreases growth rate of 3D spheroids. These effects could all be reversed by an inhibitor of FNMA and by the glucocorticoid receptor antagonist, RU-486. …”
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    Dexamethasone-Mediated Activation of Fibronectin Matrix Assembly Reduces Dispersal of Primary Human Glioblastoma Cells
  9. 4269
    “…The ICs(50) after 72 h treatment with STA-1474 were 0.08 and 0.11 μM for BACA and CLAC, respectively. When grown as spheroids, the IC(50) of STA-1474 for BACA cells was approximately two-fold higher than when grown as a monolayer (0.348 μM vs. 0.168 μM), whereas CLAC spheroids were relatively drug resistant. …”
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    Human Genetic Relevance and Potent Antitumor Activity of Heat Shock Protein 90 Inhibition in Canine Lung Adenocarcinoma Cell Lines
  10. 4270
    “…This niche was modeled using spheroids of cultured glioblastoma cells and its contribution to tumorigenicity was evaluated by sphere formation assay. …”
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    Histological Characterization of the Tumorigenic “Peri-Necrotic Niche” Harboring Quiescent Stem-Like Tumor Cells in Glioblastoma
  11. 4271
    “…Histopathological examination revealed markedly thickened Descemet's membrane with subepithelial spheroidal degeneration. SLC4A11 screening showed a novel variant p.Ser415Asn, reported mutation p.Cys386Arg and two polymorphisms, all in the heterozygous state and not identified in 100 controls. …”
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    Delayed onset of congenital hereditary endothelial dystrophy due to compound heterozygous SLC4A11 mutations
  12. 4272
    “…Therefore, in the present study, we analyzed the invasive behavior of mixed N-type and S-type multicellular spheroids embedded in three-dimensional collagen gels. …”
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    Cooperative cell invasion: matrix metalloproteinase–mediated incorporation between cells
  13. 4273
    “…In this study, we screened several polymers for their ability to suspend cells or cell spheroids homogeneously in a liquid medium without changing the viscosity behavior, and identified gellan gum (FP001), as the most potent polymer. …”
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    Novel 3‐D cell culture system for in vitro evaluation of anticancer drugs under anchorage‐independent conditions
  14. 4274
    “…Replacement of the TM4SF1 or TM4SF4 C-terminus with that of TM4SF5 increased spheroids growth, transwell migration, and invasive dissemination from spheroids in 3D collagen gels. …”
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    Differential regulation of cellular functions by the C-termini of transmembrane 4 L six family proteins in 2- or 3-dimensional environment
  15. 4275
    “…MATERIALS AND METHODS: In this experimental study, the human prostate cancer cell line DU145 was cultured as 300 µm diameter spheroids. We divided the spheroids into group I: control, group II: HT at 43˚C for 30 minutes (HT), group III: 4 Gy irradiation with 6 MV X-ray [RT (6 MV)], group IV: 4 Gy irradiation with 15 MV X-ray [RT (15 MV)], group V: HT+RT (6 MV), group VI: HT+RT (15 MV), group VII: RT (6 MV)+HT, and group VIII: RT (15 MV)+HT. …”
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    Enhanced DNA Damages of Human Prostate Cancer Cells Induced by Radiofrequency Capacitive Hyperthermia Pre- and Post X-rays: 6 MV versus 15 MV
  16. 4276
    “…An attachment model of human choriocarcinoma (JAr) cell spheroids to receptive-phase endometrial epithelial cells and Ishikawa cells treated with different estradiol (10(−9) M or 10(−7) M) concentrations was developed. …”
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    Serum estradiol levels in controlled ovarian stimulation directly affect the endometrium
  17. 4277
    “…Results: Due to 3D structure, the tumor spheroids were more resistant to chemotherapy compared to monolayer culture. …”
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    Novel Doxorubicin Derivatives: Synthesis and Cytotoxicity Study in 2D and 3D in Vitro Models
  18. 4278
    “…It has been shown that aggregation of MSCs into 3D spheroids, as a preconditioning strategy, can enhance immunomodulatory potential of such cells. …”
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    Secretome of Aggregated Embryonic Stem Cell-Derived Mesenchymal Stem Cell Modulates the Release of Inflammatory Factors in Lipopolysaccharide-Induced Peripheral Blood Mononuclear Cells
  19. 4279
    “…Moreover, TLR2 stimulation of MC promoted stronger growth of colon cancer spheroids. By examining the transcriptome profile of colon cancer-cocultured MC versus control MC, we identified several MC marker genes, which were deregulated in expression. …”
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    Human mast cells promote colon cancer growth via bidirectional crosstalk: studies in 2D and 3D coculture models
  20. 4280
    “…We analyzed the effects of CPX on three-dimensional (3D) esophageal tumor cell spheroids. We also analyzed effects on cell cycle by flow cytometry. …”
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    3508 Ciclopirox Olamine Demonstrates Inhibitory Effects on Esophageal Tumor Cells
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