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4521por Boutin, Molly E., Voss, Ty C., Titus, Steven A., Cruz-Gutierrez, Kennie, Michael, Sam, Ferrer, Marc“…Here we report the first high-throughput protocol for optical clearing of spheroids, fluorescent high-content confocal imaging, 3D nuclear segmentation, and post-segmentation analysis. …”
Publicado 2018
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4522por Zhu, Yanfang, Lu, Yuewen, Gao, Bing, Wang, Dunju, Guo, Changping, Yang, Guangcheng“…At present, there are several ways to reduce the sensitivity of CL-20, such as spheroidization, ultrafine processing, and composite technology. …”
Publicado 2018
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4523por Ding, Naiqing, Zou, Zhengyun, Sha, Huizi, Su, Shu, Qian, Hanqing, Meng, Fanyan, Chen, Fangjun, Du, Shiyao, Zhou, Shujuan, Chen, Hong, Zhang, Lianru, Yang, Ju, Wei, Jia, Liu, Baorui“…In this study, we demonstrate for the first time that iRGD could also facilitate the infiltration of lymphocytes in both 3D tumor spheroids and several xenograft mouse models. In addition, combining iRGD modification with PD-1 knockout lymphocytes reveals a superior anti-tumor efficiency. …”
Publicado 2019
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4524“…The major approaches highlighted here include cell spheroids, hydrogel embedding, bioreactors, scaffolds, and bioprinting. …”
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4525por Snyder, Glen T., Matsumoto, Ryo, Suzuki, Yohey, Kouduka, Mariko, Kakizaki, Yoshihiro, Zhang, Naizhong, Tomaru, Hitoshi, Sano, Yuji, Takahata, Naoto, Tanaka, Kentaro, Bowden, Stephen A., Imajo, Takumi“…Our research documents the discovery of spheroidal microdolomite aggregates found in association with other impurities inside of these massive gas hydrates. …”
Publicado 2020
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4526“…In particular, this review highlights studies on multicellular spheroids and other 3D tissue engineering approaches for cancer drug development. …”
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4527por Tamanoi, Fuyuhiko, Matsumoto, Kotaro, Doan, Tan Le Hoang, Shiro, Ayumi, Saitoh, Hiroyuki“…The ability of Gd–MSN to inhibit tumor growth was demonstrated by evaluating effects of irradiating tumor spheroids with a precisely tuned monochromatic X-ray.…”
Publicado 2020
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4528por Nassef, Mohamed Zakaria, Melnik, Daniela, Kopp, Sascha, Sahana, Jayashree, Infanger, Manfred, Lützenberg, Ronald, Relja, Borna, Wehland, Markus, Grimm, Daniela, Krüger, Marcus“…In addition, through the de novo generation of tumor spheroids, µg research provides a reliable in vitro 3D tumor model for preclinical cancer drug development and to study various processes of cancer progression. …”
Publicado 2020
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4529por Maruno, Takahisa, Fukuda, Akihisa, Goto, Norihiro, Tsuda, Motoyuki, Ikuta, Kozo, Hiramatsu, Yukiko, Ogawa, Satoshi, Nakanishi, Yuki, Yamaga, Yuichi, Yoshioka, Takuto, Takaori, Kyoichi, Uemoto, Shinji, Saur, Dieter, Chiba, Tsutomu, Seno, Hiroshi“…Using genetic lineage tracing with a dual-recombinase system and live imaging, we showed that Dclk1(+) tumor cells continuously provided progeny cells within pancreatic intraepithelial neoplasia, primary and metastatic PDAC, and PDAC-derived spheroids in vivo and in vitro. Furthermore, genes associated with CSC and epithelial mesenchymal transition were enriched in mouse Dclk1(+) and human DCLK1-high PDAC cells. …”
Publicado 2021
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4530por Deshmukh, Rashmi, Reddy, Jagadesh C, Rapuano, Christopher J, Vaddavalli, Pravin K“…Some of the commonly treated conditions include recurrent corneal erosions (RCE), corneal dystrophies, spheroidal degeneration, keratoconus, and corneal scars. …”
Publicado 2020
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4531por Moritoki, Yukihito, Furukawa, Taichi, Sun, Jinyi, Yokoyama, Minoru, Shimono, Tomoyuki, Yamada, Takayuki, Nishiwaki, Shinji, Kageyama, Tatsuto, Fukuda, Junji, Mukai, Masaru, Maruo, Shoji“…The development of handling technology for microscopic biological samples such as cells and spheroids has been required for the advancement of regenerative medicine and tissue engineering. …”
Publicado 2021
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4532por Schott, Dominik Jan, Saphala, Addythia, Fischer, Georg, Xiong, Wenxin, Gabbrielli, Andrea, Bordoy, Joan, Höflinger, Fabian, Fischer, Kai, Schindelhauer, Christian, Rupitsch, Stefan Johann“…The first method is Direct Intersection, which determines a coordinate point based on the intersection of spheroids defined by observed distances of high-intensity reverberations. …”
Publicado 2021
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4533por De Groof, Timo W. M., Bergkamp, Nick D., Heukers, Raimond, Giap, Truc, Bebelman, Maarten P., Goeij-de Haas, Richard, Piersma, Sander R., Jimenez, Connie R., Garcia, K. Christopher, Ploegh, Hidde L., Siderius, Marco, Smit, Martine J.“…By displacing Gα(q) protein, VUN103 prevents US28 signaling and reduces tumor spheroids growth. Overall, nanobodies specific for distinct GPCR conformational states, i.e. apo- and agonist-bound, can selectively target and discern functional consequences of ligand-dependent versus independent signaling.…”
Publicado 2021
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4534“…Here, we demonstrate that spherical self-beating CM aggregates, termed cardiac spheroids (CSs), produce enhanced mechanical energy under mechanical compression and work cooperatively via mechanical communication. …”
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4535por Vasquez, Claudia G., Vachharajani, Vipul T., Garzon-Coral, Carlos, Dunn, Alexander R.“…Here we use live-cell imaging to study the physical mechanism of lumen formation in Madin-Darby Canine Kidney cell spheroids, a canonical cell-culture model for lumenogenesis. …”
Publicado 2021
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4536por Hachey, Stephanie J., Movsesyan, Silva, Nguyen, Quy H., Burton-Sojo, Giselle, Tankazyan, Ani, Wu, Jie, Hoang, Tuyen, Zhao, Da, Wang, Shuxiong, Hatch, Michaela M., Celaya, Elizabeth, Gomez, Samantha, Chen, George T., Davis, Ryan T., Nee, Kevin, Pervolarakis, Nicholas, Lawson, Devon A., Kessenbrock, Kai, Lee, Abraham P., Lowengrub, John, Waterman, Marian L., Hughes, Christopher C. W.“…Here we have validated this microphysiological system (MPS) platform for the study of colorectal cancer (CRC), the second leading cause of cancer-related deaths, by showing that gene expression, tumor heterogeneity, and treatment responses in the VMT more closely model CRC tumor clinicopathology than current standard drug screening modalities, including 2-dimensional monolayer culture and 3-dimensional spheroids.…”
Publicado 2021
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4537por Skvortsova, Nina N., Stepakhin, Vladimir D., Sorokin, Andrey A., Malakhov, Dmitry V., Gusein-zade, Namik G., Akhmadullina, Nailya S., Borzosekov, Valentin D., Voronova, Elena V., Shishilov, Oleg N.“…The effect of the levitating cloud on stainless steel plates is studied and it is shown that regolith particles in the shape of spheroids of different sizes are deposited on the surface of the plates. …”
Publicado 2021
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4538por Hu, Banglian, Duan, Shengshun, Wang, Ziwei, Li, Xin, Zhou, Yuhang, Zhang, Xian, Zhang, Yun-Wu, Xu, Huaxi, Zheng, Honghua“…CSF1R loss-of-function mutations are the major cause of adult-onset leukoencephalopathy with axonal spheroids and pigmented glia (ALSP) and its dysfunction has also been implicated in other neurodegenerative disorders including Alzheimer’s disease (AD). …”
Publicado 2021
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4539por Bonelli, Joaquín, Ortega-Forte, Enrique, Rovira, Anna, Bosch, Manel, Torres, Oriol, Cuscó, Cristina, Rocas, Josep, Ruiz, José, Marchán, Vicente“…Importantly, this nanoformulation impaired tumoral growth of clinically relevant three-dimensional multicellular tumor spheroids. Mitochondrial photodamage along with reactive oxygen species (ROS) photogeneration was found to trigger autophagy and apoptotic cell death of cancer cells.…”
Publicado 2022
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4540por Waibel, Dominik J.E., Kiermeyer, Niklas, Atwell, Scott, Sadafi, Ario, Meier, Matthias, Marr, Carsten“…Applied to 2D images containing spheroidal aggregates of densely grown human induced pluripotent stem cells, we find that SHAPR learns fundamental shape properties of cell nuclei and allows for prediction-based morphometry. …”
Publicado 2022
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