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441por Rizzo, Riccardo, Onesto, Valentina, Morello, Giulia, Iuele, Helena, Scalera, Francesca, Forciniti, Stefania, Gigli, Giuseppe, Polini, Alessandro, Gervaso, Francesca, del Mercato, Loretta L.“…Moreover, a pH gradient distribution was detected in the untreated spheroids, with more acidic values in proximity of the spheroids, resembling the cell metabolic features observed in vivo in the tumor microenvironment. …”
Publicado 2023
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442por Gwon, Kihak, Choi, Daheui, de Hoyos-Vega, José M., Baskaran, Harihara, Gonzalez-Suarez, Alan M., Lee, Seonhwa, Hong, Hye Jin, Nguyen, Kianna M., Dharmesh, Ether, Sugahara, Go, Ishida, Yuji, Saito, Takeshi, Stybayeva, Gulnaz, Revzin, Alexander“…This paper describes the use of bioactive heparin-based core-shell microcapsules to form and cultivate hepatocyte spheroids. These microcapsules are comprised of an aqueous core that facilitates hepatocyte aggregation into spheroids and a heparin hydrogel shell that binds and releases growth factors. …”
Publicado 2023
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443por Lim, Ga-Hyun, An, Ju-Hyun, Park, Su-Min, Youn, Ga-Hee, Oh, Ye-In, Seo, Kyoung-Won, Youn, Hwa-Young“…A hybrid spheroid was created using an ultralow-adhesion plate. …”
Publicado 2023
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444por Akshay, Akshay, Katoch, Mitali, Abedi, Masoud, Besic, Mustafa, Shekarchizadeh, Navid, Burkhard, Fiona C., Bigger-Allen, Alex, Adam, Rosalyn M., Monastyrskaya, Katia, Gheinani, Ali Hashemi“…However, the use of 3D spheroid assays is impeded by the absence of automated and user-friendly tools for spheroid image analysis, which adversely affects the reproducibility and throughput of these assays. …”
Publicado 2023
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445por McCabe, Samantha M., Wallace, Gregory Q., Sloan-Dennison, Sian, Tipping, William J., Shand, Neil C., Graham, Duncan, Boyd, Marie, Faulds, Karen“…In this work, the uptake, distribution, and location of silica coated gold nanoparticles (AuNP-SHINs) within multicellular tumour spheroids (MTS) derived from U87-MG glioblastoma cells was investigated by surface enhanced Raman scattering (SERS) optical mapping. …”
Publicado 2023
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446por Pandey, Mrinal, Suh, Young Joon, Kim, Minha, Davis, Hannah Jane, Segall, Jeffrey E, Wu, Mingming“…Cells within the malignant spheroids became more motile within the spheroids and invaded more into the ECM under compression; whereas cells within non-tumorigenic MCF10A spheroids became less motile within the spheroids and did not display apparent detachment from the spheroids under compression. …”
Publicado 2023
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447“…After 12 days in culture, the hepatocyte spheroids exhibited biometric and morphological stability. …”
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448por Cai, Grace, Li, Xinzhi, Lin, Shan-Shan, Chen, Samuel, Koning, Katherine, Bi, Dapeng, Liu, Allen P.“…Using this hydrogel system and computational Self-Propelled Voronoi modeling, we show that spheroid sorting and invasion into the matrix depend on the balance between cell-generated forces and matrix resistance. …”
Publicado 2023
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449“…MDA-MB-231 cancer cell spheroids are embedded in Matrigel, induced to invade, and processed to image mRNAs with single-molecule sensitivity. …”
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450“…As an in-vitro tissue model for drug testing, cellular spheroids were derived from a human hepatoma cell line (i.e., HepG2 cells) using the Hanging-drop method. …”
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451“…This review article critically discusses the suitability of 3D spheroid models in oncological research. ABSTRACT: Advanced tissue engineering processes and regenerative medicine provide modern strategies for fabricating 3D spheroids. …”
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452“…Tumor spheroids in the ascites of high-grade serous carcinoma (HGSC) are poorly described. …”
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453por Akshay, Akshay, Katoch, Mitali, Abedi, Masoud, Shekarchizadeh, Navid, Besic, Mustafa, Burkhard, Fiona C, Bigger-Allen, Alex, Adam, Rosalyn M, Monastyrskaya, Katia, Gheinani, Ali Hashemi“…However, the use of 3D spheroid assays is impeded by the absence of automated and user-friendly tools for spheroid image analysis, which adversely affects the reproducibility and throughput of these assays. …”
Publicado 2023
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454“…The increasing popularity of 3D cell culture models is being driven by the demand for more in vivo-like conditions with which to study the biochemistry and biomechanics of numerous biological processes in health and disease. Spheroids and organoids are 3D culture platforms that self-assemble and regenerate from stem cells, tissue progenitor cells or cell lines, and that show great potential for studying tissue development and regeneration. …”
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455por Osete, Jordi Requena, Akkouh, Ibrahim A., Ievglevskyi, Oleksandr, Vandenberghe, Matthieu, de Assis, Denis Reis, Ueland, Thor, Kondratskaya, Elena, Holen, Børge, Szabo, Attila, Hughes, Timothy, Smeland, Olav B., Steen, Vidar Martin, Andreassen, Ole A., Djurovic, Srdjan“…We studied the effects of 1 mM Li treatment for 1 month in iPSC-derived human cortical spheroids (hCS) from 10 healthy controls (CTRL) and 11 BD patients (6 Li-responders, Li-R, and 5 Li non-treated, Li-N). …”
Publicado 2023
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456por Abbasi Aval, Negar, Lahchaichi, Ekeram, Tudoran, Oana, Fayazbakhsh, Farzaneh, Heuchel, Rainer, Löhr, Matthias, Pettersson, Torbjörn, Russom, Aman“…However, the creation of these tumor spheroids presents challenges, given that not all tumor cell lines are able to form consistent and regular spheroids. …”
Publicado 2023
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457por Strong, Caroline E., Zhang, Jiajing, Carrasco, Martin, Kundu, Srikanya, Boutin, Molly, Vishwasrao, Harshad D., Liu, Jiamin, Medina, Angelica, Chen, Yu-Chi, Wilson, Kelli, Lee, Emily M., Ferrer, Marc“…3D spheroids have emerged as powerful drug discovery tools given their high-throughput screening (HTS) compatibility. …”
Publicado 2023
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458“…Cells from the centre of EMT-6 spheroids had increased activity of the Na+/H+ exchanger compared to cells from the periphery, when spheroids were grown in medium at pH 6.6, but not at 7.4. …”
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459“…The necrotic cores of the initially avascular spheroids were replaced by vascularised tumour tissue but the outer zones of the spheroids failed to become vascularised. …”
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460“…We have compared the response to a number of cytotoxic drugs of cells treated either within intact multicellular spheroids or as isolated cells following spheroid disaggregation. …”
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