“…At intermediate flow strength, a purely configurational microphase separation was evident that manifested as a bicontinuous phase composed of regions of highly stretched molecules that enmeshed spheroidal domains of relatively coiled chains. At high flow strength, a flow-induced crystallization (FIC) occurred, producing a semicrystalline material possessing a high degree of crystallinity and primarily a monoclinic lattice structure. …”
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“…Moreover, Autophinib-treated A549 cells are unable to form spheroids, which indicates a reduction in stemness. …”
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“…RESULTS: Results indicated faster cytolysis by virus-free CRISPR-edited CAR T cells compared with retrovirally transduced CAR T cells, accompanied by increased inflammatory cytokine release, CD8+ CAR T-cell presence in co-culture conditions and CAR T-cell infiltration into three-dimensional GBM spheroids. Computational modeling identified increased tumor necrosis factor α concentrations with decreased glutamine, lactate and formate as being most predictive of short-term (2 days) and long-term (7 days) CAR T cell potency against GBM stem cells. …”
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“…Notably, the combined TP-PDT/PTT exhibited significantly improved phototherapeutic outcomes compared to individual strategies against 2D monolayer cells and 3D multicellular tumor spheroids. Overall, this study reveals a successful single-laser-triggered, synergistic combined TP-PDT/PTT based on a plasmonic metal/QD hybrid, with potential for future investigation in clinical settings.…”
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“…METHODS: We performed preclinical studies with the orally available allosteric MALT1 inhibitor (S)-mepazine as a single-agent and in combination with anti-programmed cell death protein 1 (PD-1) ICT to investigate its pharmacokinetic properties and antitumor effects in several murine tumor models as well as patient-derived organotypic tumor spheroids (PDOTS). RESULTS: (S)-mepazine demonstrated significant antitumor effects and was synergistic with anti-PD-1 therapy in vivo and ex vivo but did not affect circulating Treg frequencies in healthy rats at effective doses. …”
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“…Based on our results, the produced heterogeneous pancreatic microtissue recapitulated the features of cellular interactions and stromal-like microenvironments, and displayed better anti-cancer drug resistance than mono-cultured pancreatic cancer spheroids. Together, the DEP system possesses the ability to simply and flexibly produce GelMA hydrogel beads, providing a robust manufacturing tool for the pancreatic cancer drug screening platform fabrication. …”
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“…When cultured in three dimensional spheroids, mammalian stem cells can reproducibly self-organize a single anterior-posterior axis and sequentially differentiate into structures resembling the primitive streak and tailbud. …”
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“…Human breast cancer cells (MCF-7), in the forms of monolayer cell cultures and 3D spheroids, were studied by MTT, apoptosis, and ROS assays. …”
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“…Here, we show that effecting an MRE11 blockade using a prototypical inhibitor (Mirin) induces synthetic lethality (SL) in BRCA2-deficient ovarian cancer cells, HeLa cells, and 3D spheroids compared to BRCA2-proficient controls. Increased cytotoxicity was associated with double-strand break accumulation, S-phase cell cycle arrest, and increased apoptosis. …”
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“…With an increase in La content, the Mg(2)Sn phase in the crystal was gradually refined and spheroidized. When the content of La reached 1.5%, the tensile strength of the alloy reached 300 Mpa and the elongation reached 12.6%, i.e., 25% and 85% increases, respectively, compared to the Mg-3Sn-1Mn alloy. …”
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“…The most efficient molecule, ALK4, was selected based on its ability to inhibit both survival and migration of GBM and MB cells in 2D cultures and in 3D tumor spheroids. A fluorescent derivative was used to show the early cytosolic accumulation of ALK4 in tumor cells. …”
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“…Recent developments in cell biology have led to a strong interest in generating spheroids and organoids that better represent tissue. …”
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“…To this end, morphological changes in cell spheroids, propidium iodide and DiOC(6) uptake, quantification of reactive oxygen species (ROS) production, phosphatidylserine exposure, and cell cycle progression were evaluated by flow cytometry.…”
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“…Here, we compared brain tumor organoids (BTOs) with common models including cell lines, tumor spheroids, and patient‐derived xenografts. Different BTOs can be customized to research objectives and particular brain tumor features. …”
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“…The water-stable suspension of SiO(2)-NPs shows a zeta potential of −31.80 mV, and the homogeneously spheroidal morphology observed by TEM corroborates with the low polydispersity values (0.300). …”
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“…Moreover, phenanthroline-containing complexes caused a rapid loss of mitochondria membrane potential, ΔΨ(M), if compared to cisplatin, probably due to their cationic and lipophilic properties. On 3D tumor spheroids, 1 and 2 significantly reduced migrated area more than cisplatin, confirming an antimetastatic ability.…”
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“…With high similarity to sanguinarine, chelerythrine exhibited cytotoxic effects on multiple gastric cancer cell lines and suppressed the proliferation of tumor spheroids derived from NCI-N87 cells. Chelerythrine elevated cellular levels of reactive oxygen species (ROS) and induced endoplasmic reticulum (ER) stress. …”
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“…We also discuss the advantages and limitations of 3D cell culture models, such as spheroids, organoids and bioprinted tissues, as well as microfluidic technology. …”
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“…We have also developed the technology to mass-produce and efficiently engraft cardiomyocytes by generating cardiomyocyte spheroids and have developed devices suitable for cell transplantation. …”
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“…Capan 2 pancreas carcinoma cells, upon addition of HGF/SF, develop large hollow spheroids lined with a tight layer of polarized cells. …”
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