“…Cck2r-CreERT2(+) isthmus progenitors, but not Hdc(+) mature ECL cells, were the source of ECL cell hyperplasia during hypergastrinemia and could grow as 3-dimensional spheroids in vitro. Moreover, gastrin treatment in vitro promoted sphere formation from sorted Cck2r(+)Hdc(-) cells, and increased chromogranin A and phosphorylated- extracellular signal-regulated kinase expression in CCK2R-derived organoids. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The structural characterization has demonstrated the spheroidal shape of nanoparticles and dimensions under 50 nm, useful for biomedical studies. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The comparison of tissue slice co-cultures with spheroids vs. tissue slice tandem-cultures using tumor xenograft slices demonstrates advantages of the xenograft tandem approach. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Two CSF+/PET+ cases had a non‐AD neuropathological diagnosis, that is FTLD‐TDP type E (A3B1C1) and adult‐onset leukoencephalopathy with axonal spheroids (A1B1C0). INTERPRETATION: Our study demonstrates neuropathological underpinnings of amyloid‐β CSF/PET discordance. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Furthermore, the UCNPs@SiO(2)-NH(2)@FA/PEG/TCPP was well internalized into HeLa cells and three-dimensional HeLa spheroids, presumably due to the surface folic acid and small size in conjunction with endocytosis and the nonspecific uptake. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Here, we demonstrated in spheroids from osteosarcoma cell lines that the exposure to acidosis remarkably caused intracellular lipid droplets accumulation. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The current work also defined promising second-generation epigenetic drug combinations with enhanced synergy and efficacy, especially in metastasis-lineage colon cancer cells cultured as 3D spheroids and xenografts. This investigation adds to the growing interest in combination approaches that target epigenetic ‘readers’, ‘writers’, and ‘erasers’ that are deregulated in cancer and other pathologies, providing new avenues for precision oncology and cancer interception.…”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…To date, it has been reported that Fx, its metabolite of fucoxanthinol (FxOH) and Fx-rich algal extracts exerted anticancer potentials in human CRC cell lines, their cancer stem-cells-like spheroids and CRC animal models through a number of molecular mechanisms. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…In multicellular tumor spheroids, we observed strong binding-site barriers in combination with low apparent diffusion coefficients of 1 µm(2)·s(−1) and 2 µm(2) ·s(−1) for EpCAM- and HER2-binding DARPin, respectively. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Finally, MRIA9 treatment enhances the sensitivity to paclitaxel in 3D-spheroids derived from ovarian cancer cell lines and ovarian cancer patients. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…We confirmed the SAL-mediated gene repression of hTERT in PCa cell lines, native human PCa samples derived from patients treated ex vivo, as well as in cancer spheroids derived from androgen-dependent or castration resistant PCa (CRPC) cells. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Cells were grown in monolayer or as 3D multicellular spheroids, which mimic the in vivo avascular tumor architecture and physiology with a heterogeneous cell population of proliferating cells in the rim and non-cycling or necrotic cells in the core region. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…TCGA data analyses indicated that patients with low expression of Hh/EMT-related genes together with active autophagy flux tended to have a better prognosis and this correlates with a more effective response to platinum therapy. In in vitro 3D spheroids, LPA upregulated BMI-1, downregulated autophagy and inhibited platinum toxicity while RV and Hh inhibitors restored autophagy and favored BAX-mediated cell death in response to platinum. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…When in suspension/PEG-hydrogel and irradiated with UV light, the prepared o-NBA/HAase-HSA-NPs clearly reduced the pH of the surrounding medium to as low as 5.0 by producing protons and were better able to break down HA-based tumor cell spheroids (AsPC-1) and HA-hydrogel/microgels, presumably due to the enhanced HA activity at a more optimal pH. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Respondents reported on their field of cancer research; type of cancers studied; use of two-dimensional (2D)/monolayer, 2.5D and/or 3D cultures; if using co-cultures, the cell types(s) they co-culture; if using 3D cultures, whether these involve culturing the cells in a particular way to generate spheroids, or if they use additional supports/scaffolds; techniques used to analyze the 2D/2.5D/3D; and their downstream applications. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Conditioned medium disrupted the connections between cancer cells, so the spheroids in three-dimensional configuration lost their order and dispersed. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Further investigation into the physiological model of PDAC, obtained by a co-culture of tumor spheroids and cancer-associated fibroblast (CAF), highlighted that the micellar system enhanced the antitumor potential of Gem, and was demonstrated to overcome the TME-dependent drug resistance. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Methods: Pre-labelled spheroids derived from colon cancer cell lines and primary organoids derived from four colorectal cancer surgical specimens, which expressed different graded levels of CEA, were exposed in cocultures to T lymphocytes. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Sixty-four mice with metastatic prostate cancer, induced by intraprostatic injection of three-dimensional prostate cancer spheroids, were randomized into two groups: one group received resection of their primary tumor while the other group received a sham operation. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Moreover, 30 µM of 6-shogaol blocked the formation of vascular endothelial growth factor (VEGF)-induced endothelial sprouts from HUVEC spheroids and from murine aortic rings. Importantly, this study shows for the first time that 6-shogaol exhibits a vascular-disruptive impact on angiogenic sprouts from murine aortae. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso