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41“…SIMPLE SUMMARY: Cell cultures can be carried out in three dimensions (3D). Organoids and spheroids are different 3D cell culture models that can be cultured with different techniques. …”
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42por Waldherr, Linda, Seitanidou, Maria, Jakešová, Marie, Handl, Verena, Honeder, Sophie, Nowakowska, Marta, Tomin, Tamara, Karami Rad, Meysam, Schmidt, Tony, Distl, Joachim, Birner‐Gruenberger, Ruth, von Campe, Gord, Schäfer, Ute, Berggren, Magnus, Rinner, Beate, Asslaber, Martin, Ghaffari‐Tabrizi‐Wizsy, Nassim, Patz, Silke, Simon, Daniel T., Schindl, Rainer“…Most strikingly, GemIP‐mediated GEM delivery leads to the overt disintegration of targeted GBM tumor spheroids. Electrically‐driven chemotherapy, here exemplified, has the potential to radically improve the efficacy of GBM adjuvant chemotherapy by enabling exquisitely‐targeted and controllable delivery of drugs irrespective of whether these can cross the BBB.…”
Publicado 2021
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43por Valdoz, Jonard Corpuz, Jacobs, Dallin J., Cribbs, Collin G., Johnson, Benjamin C., Hemeyer, Brandon M., Dodson, Ethan L., Saunooke, Jordan A., Franks, Nicholas A., Poulson, Peter Daniel, Garfield, Seth R., Knight, Connor J., Van Ry, Pam M.“…We successfully maintained primary-derived spheroids from murine and porcine lungs in the hydrogel dish. …”
Publicado 2021
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44“…In mathematical biology, solid tumor spheroids are often studied as preliminary in vitro models of avascular tumors. …”
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45por Abidine, Yara, Giannetti, Arianna, Revilloud, Jean, Laurent, Valérie M., Verdier, Claude“…This method could be an attractive way to control their properties in the future, based on the similarity of spheroids with in vivo tumor models.…”
Publicado 2021
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46“…To improve the therapeutic effect of cell spheroids even further, various biomaterials (e.g., nano- or microparticles, fibers, and hydrogels) have been developed for spheroid engineering. …”
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47por Bissoyi, Akalabya, Tomás, Ruben M. F., Gao, Yanan, Guo, Qiongyu, Gibson, Matthew I.“…In a model toxicology challenge, the thawed spheroids matched the performance of fresh spheroids. …”
Publicado 2023
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48por Barrow, Rhiannon, Wilkinson, Joseph N., He, Yichen, Callaghan, Martin, Brüning-Richardson, Anke, Dunning, Mark, Stead, Lucy F.“…Spheroids and organoids are increasingly popular three-dimensional (3D) cell culture models. …”
Publicado 2022
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49por Ivanov, Delyan P., Parker, Terry L., Walker, David A., Alexander, Cameron, Ashford, Marianne B., Gellert, Paul R., Garnett, Martin C.“…Three-dimensional cell culture has many advantages over monolayer cultures, and spheroids have been hailed as the best current representation of small avascular tumours in vitro. …”
Publicado 2014
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50por Chen, Wenjin, Wong, Chung, Vosburgh, Evan, Levine, Arnold J., Foran, David J., Xu, Eugenia Y.“…This study presents a high-throughput image analysis software application – SpheroidSizer, which measures the major and minor axial length of the imaged 3D tumor spheroids automatically and accurately; calculates the volume of each individual 3D tumor spheroid; then outputs the results in two different forms in spreadsheets for easy manipulations in the subsequent data analysis. …”
Publicado 2014
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51por Madhavan, Mathangi, Jaiswal, Devina, Karlberg, Sarah, Duggan, Alexis, Almarshad, Hassan A., Claffey, Kevin P., Hoshino, Kazunori“…Multicellular cancer spheroids are an in vitro tissue model that mimics the three-dimensional microenvironment. …”
Publicado 2023
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54“…Here, we introduce a new cell‐printing process, fabricating cell‐spheroids and cell‐loaded constructs together without preparation of cell‐spheroids in advance. …”
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55por Yang, Panhui, Wu, Lei, Zhang, Guoyuan, Ge, Yuqing, Liu, Ting, Mao, Hongju, Zhao, Jianlong“…Cancer cell spheroids have been shown to mimic in vivo tumour microenvironment and are therefore suitable for in vitro drug screening. …”
Publicado 2023
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56por Alamán-Díez, Pilar, Borau, Carlos, Guerrero, Pedro Enrique, Amaveda, Hippolyte, Mora, Mario, Fraile, José María, García-Gareta, Elena, García-Aznar, José Manuel, Pérez, María Ángeles“…Our study explores the role of collagen type I in cancer development and spread using engineered tumor models like multicellular spheroids grown in collagen-based hydrogels to simulate early tumor formation. …”
Publicado 2023
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57por Liu, Jie, Zhang, Ying, van Dongen, Kim, Kennedy, Chris, Schotman, Maaike J.G., Marín San Román, Patricia P., Storm, Cornelis, Dankers, Patricia Y.W., Sijbesma, Rint P.“…Both carbohydrate ligands used are proposed to bind to asialoglycoprotein receptors (ASGPRs) in hepatic cells, thus inducing spheroid formation when seeding hepatic HepG2 cells on both supramolecular hydrogels. …”
Publicado 2023
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58“…Multicellular spheroids are useful models for drug testing or studying tumor biology, but their production requires specialized approaches. …”
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59por Vakhshiteh, Faezeh, Bagheri, Zeinab, Soleimani, Marziye, Ahvaraki, Akram, Pournemat, Parisa, Alavi, Seyed Ebrahim, Madjd, Zahra“…Heterotypic 3D tumor spheroids, composed of multiple cell types, have become more popular than homotypic spheroids, which consist of a single cell type, as a superior model for mimicking in vivo tumor heterogeneity and physiology. …”
Publicado 2023
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60“…Biomaterials offer numerous opportunities to preserve spheroid function and guide spheroid behavior by tailoring the local microenvironment.…”
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