“…By varying the concentrations of these DNA probes and their incubation time, we have first characterized the kinetics and efficiency of probe penetration and loading onto tumor spheroids and stem cell EBs of different sizes. After optimization, we have further imaged and measured E-cadherin-mediated forces in these 3D spheroids and EBs for the first time. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…RESULTS: We developed heterotypic spheroids characterized by a mixed phenotype of evenly distributed macrophages. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Mesenchymal stem cell (MSC) spheroids were included in the injectable PMMA‐doped gel (= PMMA‐doped spheroid gel). …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…We focused on the molecular activation of the mechanosignaling pathways previously associated with spheroid formation in microgravity. Our results suggest that RPM-induced spheroid formation is a two-step process. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Here we have developed an in vitro platform to rapidly establish co-cultures of patient-derived leukaemia cells with 3D bone marrow mesenchyme spheroids, BM-MSC-spheroids.  We optimise protocols for developing MSC-spheroid leukaemia co-culture using clinical samples and deliver drug response data within a week. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…BACKGROUND: Considering the width and importance of using Multicellular Tumor Spheroids (MTS) in oncology research, size determination of MTSs by an accurate and fast method is essential. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…BACKGROUND: Multicellular spheroids, an appropriate in vitro system for simulating 3-D tumor micro-milieu can be used for evaluating and predicting tumor response to therapeutic agents including metabolic inhibitors. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Chinese hamster cells (V79 379A) cells from a human small cell carcinoma of the lung (ME/MAR) and two xenografted human melanomas (HX117 and HX118) have been grown as multicellular spheroids in vitro. The radiation response of these four cell types has been compared when grown as spheroids (200 or 400 micron in diameter) and as single cells from disaggregated spheroids. …”
Enlace del recurso
Enlace del recurso

“…Generally these results mirrored the cellular uptake in vitro. When WiDr spheroids were increased in size from 250 microns to 500 microns there was a reduction in uptake of AlS1Pc and AlS2Pc which was reflected by the decreased sensitivity of the larger spheroids to the effects of light irradiation. …”
Enlace del recurso
Enlace del recurso

“…The spheroids were grown in liquid-overlay culture for up to 6 passages. …”
Enlace del recurso
Enlace del recurso

“…Using growth delay and clonogenic cell survival as end points, we have shown that the 3-dimensional structure of human lung tumour spheroids confers a degree of resistance to the anthracyclines adriamycin and 4'-deoxydoxorubicin, relative to cells grown as monolayer. 4'-deoxydoxorubicin induces a longer growth delay and greater clonogenic cell kill than adriamycin in spheroids, although it is no more cytotoxic in monolayer (exponential and plateau phase). …”
Enlace del recurso
Enlace del recurso

Enlace del recurso
Enlace del recurso

“…When thioguanine-resistant V79 cells are introduced into spinner flasks containing V79 multicell spheroids, the mutant cells attach to the surface of the spheroids. …”
Enlace del recurso
Enlace del recurso

“…Autoradiographic identification of labelled cells indicated that nuclear incorporation of [125I]IUdR decreased markedly with increasing size of spheroid. IUdR incorporation was maximal in the surface layer of cells and decreased with depth within spheroids. …”
Enlace del recurso
Enlace del recurso

“…Liver cells isolated from newborn rats and seeded on a non-adherent plastic substratum were found to spontaneously re-aggregate and to form, within a few days, spheroidal aggregates that eventually reached a plateaued diameter of 150-175 micron. …”
Enlace del recurso
Enlace del recurso

“…The effects of electrical direct current (DC) field pulses on c-fos expression, growth kinetics and vitality patterns of multicellular tumour spheroids (MCSs) were studied. Monitoring the membrane potential of MCSs by di-8-ANNEPS staining and confocal microscopy during DC electrical field treatment revealed a hyperpolarization at the anode-facing side and a depolarization at the cathode-facing side. …”
Enlace del recurso
Enlace del recurso

“…In Matrigel they formed smooth and irregular spheroids, which had a lumen surrounded by a single cell layer. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…We examined claudin 4 expression in ovarian cancer tissues and cell lines, as well as by immunohistochemical staining of tissue microarrays (TMAs; n = 500), spheroids present in patients’ ascites, and spheroids formed in vitro. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Multicellular tumour spheroid (MCTS) cultures are excellent model systems for simulating the development and microenvironmental conditions of in vivo tumour growth. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Compared with their parental counterparts, spheroid cells demonstrated higher capacity of invasion, higher tumorigenic and metastatic potential. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso