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841por Li, LeeAnn K., Huang, Wen-Chin, Hsueh, Yuan-Yu, Yamauchi, Ken, Olivares, Natalie, Davila, Raul, Fang, Jun, Ding, Xili, Zhao, Weikang, Soto, Jennifer, Hasani, Mahdi, Novitch, Bennett, Li, Song“…MSC spheroids, on the other hand, had insignificant effect. …”
Publicado 2022
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843“…RNA sequencing was performed to identify the transcriptomic profiles of ASC spheroids and sheets relative to monolayer ASCs. By transferring ASC spheroids onto ASC sheets, the spheroid sheet composites could be successfully fabricated after a short-term co-culture, and their angiogenic potential was evaluated in vitro and in ovo. …”
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844por Sharma, Poonam, Beck, Dominik, Murtha, Lucy A., Figtree, Gemma, Boyle, Andrew, Gentile, Carmine“…In this study, we generated and compared in vitro cardiac spheroids (CSs) from wild type (WT) and fibulin-3 knockout (Fib-3 KO) mice. …”
Publicado 2022
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845por Pat, Yagiz, Rückert, Beate, Ogulur, Ismail, Yazici, Duygu, Pérez‐Diego, Mario, Küçükkase, Ozan C., Li, Manru, Akdis, Cezmi A.Enlace del recurso
Publicado 2022
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846por Suraiya, Anisha B., Evtimov, Vera J., Truong, Vinh X., Boyd, Richard L., Forsythe, John S., Boyd, Nicholas R.“…Microgel recovered CAR-T cells demonstrated potent on-target cytotoxicity against human ovarian cancer in vitro and on three-dimensional tumor spheroids, by completely eliminating tumor cells. The gelatin-based micro-hydrogels have the potential to serve as carrier systems to augment CAR-T immunotherapeutic treatment of solid tumors.…”
Publicado 2022
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847por Ono, Kisho, Sato, Kohei, Nakamura, Tomoya, Yoshida, Yume, Murata, Shogo, Yoshida, Kunihiro, Kanemoto, Hideka, Umemori, Koki, Kawai, Hotaka, Obata, Kyoichi, Ryumon, Shoji, Hasegawa, Kazuaki, Kunisada, Yuki, Okui, Tatsuo, Ibaragi, Soichiro, Nagatsuka, Hitoshi, Sasaki, Akira“…Results: Each cell line formed spheroidal cell aggregates and showed enhancement of cell adhesion molecules over time. …”
Publicado 2022
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848por Lugand, Leonard, Mestrallet, Guillaume, Laboureur, Rebecca, Dumont, Clement, Bouhidel, Fatiha, Djouadou, Malika, Masson-Lecomte, Alexandra, Desgrandchamps, Francois, Culine, Stephane, Carosella, Edgardo D., Rouas-Freiss, Nathalie, LeMaoult, Joel“…Tumor spheroids play an increasingly important role in cancer research. …”
Publicado 2022
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849“…The aim of this study is to develop a fast and simple method to enable a direct analysis of whole blood creatinine with performance measures that are comparable to conventional LC–MS/MS. 5μL whole blood is formed as a three-dimensional spheroid on hydrophobic silanized paper substrates which then undergoes paper-spray ionization—tandem mass spectrometry (PSI–MS/MS). …”
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850por Zhou, Chumin, Fabbrizi, Maria Rita, Hughes, Jonathan R., Grundy, Gabrielle J., Parsons, Jason L.“…We have analysed the comparative response of in vitro 3D spheroid models of oropharyngeal squamous cell carcinoma to x-ray (photon) irradiation and provide further evidence that HPV-positive cells, in this case now grown as spheroids, show greater inherent radiosensitivity compared to HPV-negative spheroids due to defective DSB repair. …”
Publicado 2022
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851por Shi, Huaikai, Rath, Emma M., Lin, Ruby C. Y., Sarun, Kadir Harun, Clarke, Candice Julie, McCaughan, Brian C., Ke, Helen, Linton, Anthony, Lee, Kenneth, Klebe, Sonja, Maitz, Joanneke, Song, Kedong, Wang, Yiwei, Kao, Steven, Cheng, Yuen Yee“…We confirmed via SEM analysis that 3D cells grown using the spheroid methods expressed highly interconnected cell-to-cell junctions. …”
Publicado 2022
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852“…For A549/fibroblast coculture spheroids, PEG-LZ and PEG-BSA exhibited significantly decreased tissue penetration as compared with that of unmodified proteins. …”
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853“…Therefore, detailed protocols related to robust 3D-aggregated spheroid model (3D-ASM) production were optimized and proposed. …”
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854por Ida, Yosuke, Sato, Tatsuya, Umetsu, Araya, Watanabe, Megumi, Furuhashi, Masato, Hikage, Fumihito, Ohguro, Hiroshi“…The cells were subjected to a variety of analyses including lipid staining, real-time cellular metabolic analysis, the mRNA expressions of genes related to adipogenesis and extracellular matrices (ECMs) as well as the sizes and physical properties of the 3D spheroids by a micro-squeezer. BIM-A induced strong inhibitory effects on most of the adipogenesis-related changes in the 2D and 3D cultured 3T3-L1 cells, including (1) the enlargement and softening of the 3D spheroids, (2) a dramatic enhancement in lipid staining and the expression of adipogenesis-related genes, and (3) a decrease in mitochondrial and glycolytic metabolic function. …”
Publicado 2022
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855por Li, Aijun, Muenst, Simone, Hoffman, Julius, Starck, Laurent, Sarem, Melika, Fischer, Andreas, Hutter, Gregor, Shastri, V. Prasad“…Using the synthetic tumor microenvironment mimics (STEMs)—a spheroid system composed of breast cancer cells (BCC) with defined human MSC and EC fractions, here we show that EC organization into vascular structures is BC phenotype dependent, and independent of ERα expression in epithelial cancer cells, and involves MSC-mediated Notch1 signaling. …”
Publicado 2022
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856“…Prior work has explored these issues using synthetically engineered heterogeneous multicell spheroid systems, in which cell subpopulations engage in bidirectional intercellular signaling to regulate the expression of different cadherins. …”
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857“…The aim of this study was to examine the codependent interaction of the mesenchymal stroma with head and neck squamous cell carcinoma (HNSCC) in a 3D spheroid structure. To simulate stroma-rich and -poor 3D tumor microenvironments, cells of the established cell SCC-040 were cultured with human mesenchymal stromal cells (MSCs), forming 3D stroma-tumor spheroids (STSs). …”
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859“…Alkaline phosphatase activity assays were designed to measure the osteogenic differentiation of mesenchymal stem cell spheroids on Day 7. Alizarin Red S staining was performed to investigate the mineralization of cell spheroids on Days 7 and 14. …”
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860por Carrillo, David, Reggiardo, Roman E., Lim, John, Mantalas, Gary, Peddu, Vikas, Kim, Daniel H.“…We analyzed the transcriptomes of 3D human lung cancer spheroids that harbor KRAS(G12C) mutations to determine the landscape of TE RNAs regulated by mutant KRAS(G12C). …”
Publicado 2023
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