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  1. 1081
    “…In the middle of the cardiac differentiation process, spheroids were then dissociated into single cells and reaggregated into smaller spheroids using the microfabricated vessels. …”
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    A novel cardiac differentiation method of a large number and uniformly-sized spheroids using microfabricated culture vessels
  2. 1082
    “…Culturing both spheroids and MelanoDerm™ for seven days in the presence of the skin-lightening agent fucoxanthin resulted in a more significant lowering of melanin levels in spheroids. …”
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    Human Melanocyte-Derived Spheroids: A Precise Test System for Drug Screening and a Multicellular Unit for Tissue Engineering
  3. 1083
    “…Tumour spheroids have the potential to be used as preclinical chemo-sensitivity assays. …”
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    A synergic approach to enhance long-term culture and manipulation of MiaPaCa-2 pancreatic cancer spheroids
  4. 1084
    “…Here we present a fully automated pipetting robot that is able to (a) seed hanging drops from single cell suspensions, (b) treat the spheroids formed in these hanging drops with drugs and (c) analyse the viability of the spheroids by an image-based deep learning based convolutional neuronal network (CNN). …”
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    Automated spheroid generation, drug application and efficacy screening using a deep learning classification: a feasibility study
  5. 1085
    “…Typically, small-sized tumor spheroid models (100–500 μm) are used to study various biological and physicochemical activities. …”
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    Assessing Advantages and Drawbacks of Rapidly Generated Ultra-Large 3D Breast Cancer Spheroids: Studies with Chemotherapeutics and Nanoparticles
  6. 1086
    “…In addition, matrilin-3-primed Ad-MSC spheroid implantation was associated with optimal repair in a rabbit model. …”
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    Efficacy of matrilin-3-primed adipose-derived mesenchymal stem cell spheroids in a rabbit model of disc degeneration
  7. 1087
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    Corrigendum to “A Chemically Defined Serum-Free Culture System for Spontaneous Human Mesenchymal Stem Cell Spheroid Formation”
  8. 1088
    “…Rotational culture promotes primary human osteoblasts (hOBs) to form three‐dimensional (3D) multicellular spheroids with bone tissue‐like structure without any scaffolding material. …”
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    Vitamin K2 Modulates Vitamin D‐Induced Mechanical Properties of Human 3D Bone Spheroids In Vitro
  9. 1089
    “…The spheroid evolution, cell viability, and ATP levels were monitored at 24 and 48 h after the applied treatments. …”
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    Antimicrobial Peptides as New Combination Agents in Cancer Therapeutics: A Promising Protocol against HT-29 Tumoral Spheroids
  10. 1090
    “…In this work, we review the strategies of tissue engineering in the fabrication of three-dimensional (3D) models used in virology studies, which presented many advantages over conventional cell cultures, such as complex cytoarchitecture and a more physiological microenvironment. Scaffold-free (spheroids and organoids) and scaffold-based (3D scaffolding and 3D bioprinting) approach allow the biofabrication of more realistic models relevant to the pandemic, to be used as in vitro platforms for the development of new vaccines and therapies against COVID-19.…”
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    3D culture models to study SARS-CoV-2 infectivity and antiviral candidates: From spheroids to bioprinting
  11. 1091
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    Author Correction: High-throughput screening reveals higher synergistic effect of MEK inhibitor combinations in colon cancer spheroids
  12. 1092
    “…Additionally, a hydrogel with cell spheroids was much more effective than other groups in angiogenesis since the cell spheroid has the possibility of cell–cell signaling to promote vascular generation.…”
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    Enzyme-Crosslinked Gelatin Hydrogel with Adipose-Derived Stem Cell Spheroid Facilitating Wound Repair in the Murine Burn Model
  13. 1093
    “…Here, we fabricated DIPC spheroids using concave microwells, which enabled large-scale production of spheroids of the desired size. …”
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    Spheroid Fabrication Using Concave Microwells Enhances the Differentiation Efficacy and Function of Insulin-Producing Cells via Cytoskeletal Changes
  14. 1094
    “…Therefore, in this study, we aimed to establish a 3D spheroid culture using converted canine iHep cells to investigate their function as hepatocytes. …”
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    Three-dimensional spheroid culture of canine hepatocyte-like cells derived from bone marrow mesenchymal stem cells
  15. 1095
    por Kubouchi, Koji, Mukai, Hideyuki
    Publicado 2021
    “…The role of Protein Kinase N2 (PKN2, also known as PRK2/PKNγ) in cell aggregate/spheroid formation in suspension culture was investigated using immortalized fibroblasts established from PKN2(flox/flox) mouse embryos. …”
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    PKN2 is involved in aggregation and spheroid formation of fibroblasts in suspension culture by regulating cell motility and N-cadherin expression
  16. 1096
    “…FaDu spheroids were incubated with indocyanine green (ICG) and then included in a tissue-like phantom. …”
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    Validation of a Three-Dimensional Head and Neck Spheroid Model to Evaluate Cameras for NIR Fluorescence-Guided Cancer Surgery
  17. 1097
    “…The combination of pure cardiac spheroids and gelatin hydrogel succeeded to recover reduced ejection fraction. …”
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    Intramyocardial Transplantation of Human iPS Cell–Derived Cardiac Spheroids Improves Cardiac Function in Heart Failure Animals
  18. 1098
    “…Spheroid formation was demonstrated for three hPSC lines to highlight broad utility of this encapsulation technology. …”
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    Core–shell hydrogel microcapsules enable formation of human pluripotent stem cell spheroids and their cultivation in a stirred bioreactor
  19. 1099
    “…Having confirmed CSCs enrichment in spheroids, CSC(enr)‐EXOs were purified and characterized, and their impact on DC maturation was investigated. …”
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    Dendritic cells loaded with exosomes derived from cancer stem cell‐enriched spheroids as a potential immunotherapeutic option
  20. 1100
    “…RESULTS: Microarray and RNA-Seq analysis revealed that ESP-treated cholangiocyte H69 spheroids displayed global changes in gene expression compared to untreated spheroids. …”
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    Transcriptomic profiling of three-dimensional cholangiocyte spheroids long term exposed to repetitive Clonorchis sinensis excretory-secretory products
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