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  1. 1601
    “…Adipose-derived MSCs (A-MSCs) were cultured via the ordinary monolayer method and 3D hanging drop spheroid method. When the cells adhered to the wall or formed a spheroid, they were subjected to hypothermic stress at 4°C for 1 h and then divided into three recovery periods at 37°C, specifically 0, 12, and 24 h. …”
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    Cryo-Temperature Pretreatment Increases the Pro-Angiogenic Capacity of Three-Dimensional Mesenchymal Stem Cells via the PI3K-AKT Pathway
  2. 1602
    “…An in vitro cell proliferation assay demonstrated a significant decrease in cell proliferation upon asparagine depletion in the ASNS-knockdown spheroid lines, and this was not observed in the control spheroids lines. …”
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    Targeting Asparagine Synthetase in Tumorgenicity Using Patient-Derived Tumor-Initiating Cells
  3. 1603
    “…We have studied suitable conditions for the spheroid culture, such as size of spheroids and methods of stable fixing a spheroid in a dish to perform the microbeam irradiation, and observation of the cell cycles of each cell in a spheroid after irradiation using time-lapse micro-imaging technique. …”
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    Three-dimensional culture of HeLa-FUCCI cells for study of bystander cell-cycle effect of high LET particles
  4. 1604
    “…Our previous work have demonstrated that ovarian cancer spheroid cells are tumorigenic and chemo-resistant, and have the properties of ovarian CSCs. …”
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    Epithelial ovarian cancer stem-like cells expressing α-gal epitopes increase the immunogenicity of tumor associated antigens
  5. 1605
    “…The generation of human stem cell-derived spheroids and organoids represents a major step in solving numerous medical, pharmacological, and biological challenges. …”
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    Differentiation of Human Pluripotent Stem Cells Into Definitive Endoderm Cells in Various Flexible Three-Dimensional Cell Culture Systems: Possibilities and Limitations
  6. 1606
    “…Recently, the three-dimensional (3D) spheroid culture has been studied extensively. Spheroids are self-assembled cell aggregates that have biomimicry capabilities. …”
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    Different Expression of Thyroid-Specific Proteins in Thyroid Cancer Cells between 2-Dimensional (2D) and 3-Dimensional (3D) Culture Environment
  7. 1607
    “…Essentially, the conventional prolate spheroid shape model provides the simplest measurement with lowest variability of stroke volume and cardiac output. …”
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    Heart Performance Determination by Visualization in Larval Fishes: Influence of Alternative Models for Heart Shape and Volume
  8. 1608
    “…Fibroblasts and spheroid progenitors retained cell proliferation after cryopreservation. …”
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    Isolation and Culture of Skin-Derived Differentiated and Stem-Like Cells Obtained from the Arabian Camel (Camelus dromedarius)
  9. 1609
    “…Cancer spheroids are in vitro 3D models that became crucial in nanomaterials science thanks to the possibility of performing high throughput screening of nanoparticles and combined nanoparticle-drug therapies on in vitro models. …”
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    INSIDIA 2.0 High-Throughput Analysis of 3D Cancer Models: Multiparametric Quantification of Graphene Quantum Dots Photothermal Therapy for Glioblastoma and Pancreatic Cancer
  10. 1610
    “…In this paper, we developed a spheroid culture device that can trap a spheroid in the trapping site sandwiched by two extracellular matrix gels located at the upper and lower side of the spheroid. …”
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    Microfluidic Device for the Analysis of Angiogenic Sprouting under Bidirectional Biochemical Gradients
  11. 1611
    “…However, low attachment ratio and spheroid formation of several stem cell types on plain chitosan scaffolds/films is still a problem. …”
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    Scaffolds from medical grade chitosan: A good choice for 3D cultivation of mesenchymal stem cells
  12. 1612
    “…Physciosporin also decreased spheroid formation in other colorectal cancer cell lines, including DLD1, Caco2, and HT29. …”
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    Lichen Secondary Metabolite Physciosporin Decreases the Stemness Potential of Colorectal Cancer Cells
  13. 1613
    por Tobias, Fernando, Hummon, Amanda B.
    Publicado 2022
    “…We utilized serial trypsinization on the spheroid samples to generate three cellular populations representing the proliferative, quiescent, and necrotic regions of the spheroid. …”
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    Lipidomic comparison of 2D and 3D colon cancer cell culture models
  14. 1614
    “…However, to date little is known about how spheroid biology compares to that of the more traditional and widely utilized 2D monolayer cultures. …”
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    Three-Dimensional Lung Tumor Microenvironment Modulates Therapeutic Compound Responsiveness In Vitro – Implication for Drug Development
  15. 1615
    “…RESULTS: Spheroid cells showed higher potential for spheroid formation than the pa- rental cells. …”
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    STAT3 is Overactivated in Gastric Cancer Stem-Like Cells
  16. 1616
    “…Bladder volume was measured using three ultrasound methods: (1) V(spheroid): two-dimensional (2D) method calculated assuming spheroid geometry; (2) V(bih): 2D correction method obtained by multiplying V(spheroid) by a previously derived correction factor of 1.375; and (3) V(3D): three-dimensional (3D) method obtained by manually tracing the bladder outline in six planes automatically reconstructed into a solid rendered volume. …”
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    Comparison of 2D and 3D ultrasound methods to measure serial bladder volumes during filling: Steps toward development of non-invasive ultrasound urodynamics
  17. 1617
    por Nemoto, Nobuo, Sakurai, Junko
    Publicado 1993
    “…However, the ratios in spheroid culture after 48 h treatment increased, whereas they did not change in monolayer culture. …”
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    Activation of Cyp1a1 and Cyp1a2 Genes in Adult Mouse Hepatocytes in Primary Culture
  18. 1618
    “…Co-culture of SW620 or HCT116 CRC spheroids with endothelial cells suppressed spheroid growth while it had no effect on cancer cell migration or invasion. …”
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    Characterization and In Vivo Validation of a Three-Dimensional Multi-Cellular Culture Model to Study Heterotypic Interactions in Colorectal Cancer Cell Growth, Invasion and Metastasis
  19. 1619
    por Sherman, Hilary, Rossi, Ann E.
    Publicado 2019
    “…Corning spheroid microplates are cell culture microplates with round well-bottom geometry coated with Corning Ultra-low Attachment surface, enabling the formation of a single multicellular tumor spheroid centered in each well in a highly reproducible manner. …”
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    A Novel Three-Dimensional Glioma Blood-Brain Barrier Model for High-Throughput Testing of Tumoricidal Capability
  20. 1620
    “…JeKo-1-spheroid cells and the spheroid growing cells enriched from MCL patients exhibited comparably enhanced tumorigenic abilities and similar biological features. …”
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    Identification and characterization of the cellular subclones that contribute to the pathogenesis of mantle cell lymphoma
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