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  1. 1641
    “…The aim of this work was to determine how 3D spheroid growth of breast cancer cells impacts the expression and spatial organization of major acid extruding proteins, and how these proteins in turn are required for spheroid growth. …”
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    Roles of acid-extruding ion transporters in regulation of breast cancer cell growth in a 3-dimensional microenvironment
  2. 1642
    “…The LM‐based 3D stem cell spheroids are demonstrated to possess an approximately threefold of cell viability compared with the conventional spheroid obtained from nonadhesive plates. …”
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    On‐Demand Coalescence and Splitting of Liquid Marbles and Their Bioapplications
  3. 1643
    “…Among the spheroid models, we saw the best functionality in iPSC-HLC monoculture spheroids. …”
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    Improvements in Maturity and Stability of 3D iPSC-Derived Hepatocyte-like Cell Cultures
  4. 1644
    “…Therefore, more and more laboratories are tending towards multicellular spheroids grown in vitro as a model of native tissues. …”
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    High-throughput image-based monitoring of cell aggregation and microspheroid formation
  5. 1645
    “…Furthermore, 3D in vitro tumor spheroid experiments confirmed the superior effects of the combination, as the only treatment regime resulting in growth inhibition and complete spheroid disintegration. …”
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    The Stapled Peptide PM2 Stabilizes p53 Levels and Radiosensitizes Wild-Type p53 Cancer Cells
  6. 1646
    “…Compared to control ASCs, spheroid-derived ASCs seeded at the same density exhibited decreased viability in the A2-P-supplemented medium. …”
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    The Influence of Cell Culture Density on the Cytotoxicity of Adipose-Derived Stem Cells Induced by L-Ascorbic Acid-2-Phosphate
  7. 1647
    “…In the IA3D, spheroids of cancer HepG2 cells and keratinocytes HaCaT cells appeared after 2 and 3 days of culture, respectively, whereas no spheroids were formed in 2D culture. …”
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    Surface-Modified Industrial Acrylonitrile Butadiene Styrene 3D Scaffold Fabrication by Gold Nanoparticle for Drug Screening
  8. 1648
    “…Neurites and spheroid cell sizes were reduced in mutant NB cells of 2D and 3D cultures, respectively. …”
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    Functional analysis of N-acetylglucosaminyltransferase-I knockdown in 2D and 3D neuroblastoma cell cultures
  9. 1649
    “…We identified 174 out of 1280 LOPAC that significantly suppressed BeWo spheroid attachment onto endometrial Ishikawa cells. …”
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    High-Throughput In Vitro Screening Identified Nemadipine as a Novel Suppressor of Embryo Implantation
  10. 1650
    “…OBJECTIVE: To evaluate the potency of the fraction of marine sponge Stylissa carteri in inducing cell death, inhibiting spheroid growth, and its impact on pro-apoptotic protein Mcl-1S in breast cancer cells. …”
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    The Ethyl Acetate Fraction of Marine Sponge Stylissa carteri Induces Breast Cancer Cell Death via Upregulation of Mcl-1S: an In vitro Study
  11. 1651
    “…We show that Ror1 expression can be upregulated in glioblastoma cells under spheroid culture, but not adherent culture conditions. …”
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    Ror1 is expressed inducibly by Notch and hypoxia signaling and regulates stem cell‐like property of glioblastoma cells
  12. 1652
    “…In order to decipher the meaning of this spheroid structure, we established a cardiosphere clone (CSC-21E) derived from the rat heart which can switch its morphology between spheroid and nonspheroid. …”
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    Cell Shape and Cardiosphere Differentiation: A Revelation by Proteomic Profiling
  13. 1653
    “…Here we show that a tumor spheroid embedded in a 3D collagen matrix exerts large contractile forces on the matrix before invasion. …”
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    Collective cancer cell invasion induced by coordinated contractile stresses
  14. 1654
    “…Co-culture of tumor cells with glial cells had little effect on 3D spheroid formation, while treatment with cannabinoids led to significantly larger spheroids. …”
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    Interaction of Glia Cells with Glioblastoma and Melanoma Cells under the Influence of Phytocannabinoids
  15. 1655
    “…Comparative characterization of stable luciferase expressing derivatives of well-established CRC cell lines, derived spheroids and subcutaneous xenograft tumors showed that regarding primary tumor characteristics, the 3D-spheroid cultures resembled xenografts more closely than 2D-cultured cells do. …”
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    Luciferase Expressing Preclinical Model Systems Representing the Different Molecular Subtypes of Colorectal Cancer
  16. 1656
    “…Among the spheroid groups, keloid spheroids had much higher gene expression levels of collagen-1 and aSMA, which was confirmed by the immunofluorescent staining with the same correspondence proteins. …”
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    6: Biomimetic Microtissue Keloid Scar System Using Keloid-derived Fibroblasts and Macrophages
  17. 1657
    “…Since then, we have studied cellular mechanisms triggering the cells to leave a monolayer and aggregate to spheroids. Our work focused on spheroid-related changes in gene expression patterns, in protein concentrations, and in factors secreted to the culture supernatant during the period when growth is altered. …”
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    Proteome Analysis of Human Follicular Thyroid Cancer Cells Exposed to the Random Positioning Machine
  18. 1658
    “…Ovarian cancer cells can form spheroids under serum‐free suspension culture conditions. …”
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    SOX2 is required to maintain cancer stem cells in ovarian cancer
  19. 1659
    “…We reveal a consistent increase of viscosity in cancer cells leading the invasion into the collagen matrices in comparison with cancer cells following in the stalk or remaining in the center of the spheroid. We hypothesize that this differential viscoelasticity might facilitate spheroid tip invasion through a dense matrix. …”
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    Cancer cells’ ability to mechanically adjust to extracellular matrix stiffness correlates with their invasive potential
  20. 1660
    por Clark, Kendra, Janorkar, Amol V.
    Publicado 2018
    “…Three conditions were tested: uncoated tissue culture polystyrene (TCPS) surfaces that induced a 2D monolayer formation; elastin-like polypeptide (ELP)-collagen composite hydrogel scaffolds that induced encapsulated 3D spheroid culture; and ELP-polyethyleneimine-coated TCPS surfaces that induced 3D spheroid formation in scaffold-free condition. …”
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    Milieu for Endothelial Differentiation of Human Adipose-Derived Stem Cells
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