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1661por Thaweekitphathanaphakdee, Sajee, Chanvorachote, Pithi, Prateepchinda, Sagaw, Khongkow, Mattaka, Sucontphunt, Apirada“…Cell migration assay was determined using the scratch wound healing test. Spheroid formation was evaluated and the expression level of stem cell markers was investigated by western blot analysis. …”
Publicado 2019
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1662“…We hypothesized that the inclusion of peptide-based scaffold (PuraMatrix) in the spheroid matrix, to represent in vivo tumor microenvironment alterations along with metastatic site conditions, would enhance spheroid cell growth and migration and alter nanovector transport. …”
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1663por Ou, Keng-Liang, Huang, Chiung-Fang, Lan, Wen-Chien, Huang, Bai-Hung, Pan, Hsu-An, Shen, Yung-Kang, Saito, Takashi, Tsai, Hsin-Yu, Cho, Yung-Chieh, Hung, Kuo-Sheng, Chou, Hsin-Hua“…Analytical results indicated that 3BE exhibited lower spheroid formation than Matrigel. However, the 3BE appeared biocompatible to NIH 3T3, MG-63, and MCF-7 cells. …”
Publicado 2022
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1664“…By enriching LCSCs from spheroid cultures and performing transcriptomic analysis, we determined that alanine–glyoxylate aminotransferase (AGXT), which participates in the metabolism of serine and glycine, was significantly upregulated in spheroid cultures, and its function in LCSCs remains unknown. …”
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1665“…Pulsed light stimulation of an immobilized spheroid was used to simulate the light fluctuation experienced by a rotating spheroid during phototaxis. …”
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1666por Shin, Hyun-Soo, Lee, Songyi, Hong, Hye Jin, Lim, Young Chang, Koh, Won-Gun, Lim, Jae-Yol“…We determined whether salivary gland-resident stem cells (SGSCs) are primed by a 3D spheroid culture prior to treating irradiation-induced salivary hypofunction using in-vitro coculture and in-vivo transplant models. …”
Publicado 2018
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1667por Rausch, Magdalena, Blanc, Léa, De Souza Silva, Olga, Dormond, Olivier, Griffioen, Arjan W., Nowak-Sliwinska, Patrycja“…Native or immortalized immune cells were added directly or 24 h post spheroid formation, and their infiltration was observed. …”
Publicado 2021
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1668por Hoffmann, Oliver Ingo, Regenauer, Manuel, Czogalla, Bastian, Brambs, Christine, Burges, Alexander, Mayer, Barbara“…Except for treosulfan, all nonplatinum treatments tested showed a lesser effect on tumor spheroids compared to that of platinum-based therapies. …”
Publicado 2022
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1669por Siquara da Rocha, Leonardo de Oliveira, Souza, Bruno Solano de Freitas, Coletta, Ricardo Della, Lambert, Daniel W., Gurgel Rocha, Clarissa A.“…Spheroid form, size, and reproducibility were evaluated over time (Evos(TM) XL; ImageJ version 1.8). …”
Publicado 2023
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1670“…Multi-cell spheroids were grown in soft agar. When each spheroid was cultured in a large volume of medium, frequently renewed, all spheroids eventually reached a dormant phase at a diameter of approximately 3–4 mm and a population of approximately 10(6) cells. …”
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1671Rapid isolation and expansion of skin-derived precursor cells from human primary fibroblast cultures“…Multipotency was investigated by differentiation into adipocytes, smooth muscle cells and fibroblasts. The pH-SKP spheroid yield at day 5 was four- and threefold higher than those obtained using trypsin- and no-stress methods, respectively. …”
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1672“…The pancreatic CSC population, identified using cell surface markers or a tumor spheroid formation assay, was significantly reduced, with an IC(50) value of ~100 µg/ml treatment for 48 h and ~27 µg/ml for long-term tumor spheroid formation. …”
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1673por Burand, Anthony J., Di, Lin, Boland, Lauren K., Boyt, Devlin T., Schrodt, Michael V., Santillan, Donna A., Ankrum, James A.“…Local delivery is known to cause MSCs to aggregate into “spheroids,” which alters gene expression and phenotype. …”
Publicado 2020
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1674por Hu, Shiqi, Li, Zhenhua, Lutz, Halle, Huang, Ke, Su, Teng, Cores, Jhon, Dinh, Phuong-Uyen Cao, Cheng, Ke“…DP cells can partially restore their capacity to promote hair regrowth after being subjected to spheroid culture. In this study, results revealed that DP spheroids are effective at inducing the progression of the hair follicle cycle from telogen to anagen compared with just DP cell or minoxidil treatment. …”
Publicado 2020
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1675por Shirakawa, Yuki, Ohta, Kunimasa, Miyake, Shunsuke, Kanemaru, Ayumi, Kuwano, Akari, Yonemaru, Kou, Uchino, Shota, Yamaoka, Michiko, Ito, Yuki, Ito, Naofumi, Hide, Takuichiro, Shinojima, Naoki, Mukasa, Akitake, Saito, Hideyuki, Jono, Hirofumi“…Ribosome-induced cancer cell spheroid (RICCS) formation was significantly upregulated by ribosome incorporation. …”
Publicado 2021
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1676“…DNER expression was increased upon stem cell-enriching cancer spheroid culture. Knockdown of DNER expression inhibited cell proliferation and invasion, induced apoptosis, enhanced chemosensitivity, and decreased spheroid formation of SNU-638 gastric cancer cells. …”
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1677por Zscharnack, Matthias, Krause, Christoph, Aust, Gabriela, Thümmler, Christian, Peinemann, Frank, Keller, Thomas, Smink, Jeske J., Holland, Heidrun, Somerson, Jeremy S., Knauer, Jens, Schulz, Ronny M., Lehmann, Jörg“…No mice from the huChon spheroid group developed a tumor at the implantation site. …”
Publicado 2015
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1678por Jensen, Stine S., Petterson, Stine A., Halle, Bo, Aaberg-Jessen, Charlotte, Kristensen, Bjarne W.“…Siramesine caused tumor cell death and reduced secondary spheroid formation of patient-derived spheroid cultures. …”
Publicado 2017
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1679por Bikhezar, Fatima, de Kruijff, Robin M., van der Meer, Astrid J. G. M., Torrelo Villa, Guzman, van der Pol, Susanne M. A., Becerril Aragon, Gabriel, Gasol Garcia, Ana, Narayan, Ravi S., de Vries, Helga E., Slotman, Ben J., Denkova, Antonia G., Sminia, Peter“…CONCLUSION: MEK162 loaded polymersomes are taken up by multicellular spheroids. The nanocarrier delivered drug reduced spheroid growth and inhibited its molecular target. …”
Publicado 2019
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1680por Ogoke, Ogechi, Yousef, Osama, Ott, Cortney, Kalinousky, Allison, Lin, Wayne, Shamul, Claire, Ross, Shatoni, Parashurama, Natesh“…In models in which liver spheroids and MRC-5 spheroids were co-cultured, we observed complex strand morphogenesis, whereby thin, linear, 3D liver cell strands attach to the MRC-5 spheroid, anchor and thicken to form permanent and thick anchoring contacts between the two spheroids. …”
Publicado 2021
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