“…FINDINGS: Neural stem-cell marker genes, including CD44, NES, and PROM1, are generally downregulated in IDH-mutant gliomas and IDH1(R132H)-heterozygous spheroid growth compared respectively with IDH-wildtype gliomas and IDH1(R132H)-hemizygous spheroid growth, in agreement with their negative associations with patient outcome. …”
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“…Spheroid cultures of primary human hepatocytes (PHH) are used in studies of hepatic drug metabolism and toxicity. …”
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“…In this work, by using single-cell mass cytometry, we observed that stemness in spheroid-forming cells derived from MDA-MB-231 cells was significantly increased after doxorubicin administration and up-regulated integrin αvβ3 expression was also observed. …”
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“…Tumor self-renewal was detected using a spheroid formation assay and CD133 and CD44 expression assessed by flow cytometry. …”
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“…All cell lines were subjected to transwell, spheroid drop, and spheroid-gel invasion assays, and stained for vimentin, cytokeratin, E-Cadherin and N-Cadherin to assess changes in expression. …”
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“…Graphene nanosheets enhance cell proliferation and aggregation, which aids in the formation of cancer spheroids. The spheroids are assessed after day 7 and 14 for the generation of reactive oxygen species (ROS), expression of the hypoxia inducing factor (HIF-1⍺) and vascular endothelial growth factor (VEGF). …”
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“…In the bidisperse (2 and 50 kDa) spheroid with a symmetric monomer composition, local structural irregularities are probably responsible for the observed coupling between WGMs. …”
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“…The present study aimed to examine the roles and mechanisms of metformin in the stemness and epithelial-mesenchymal transition (EMT) of colorectal cancer cells. The formation of spheroid cells, and the results of reverse transcription-quantitative polymerase chain reaction and western blot analyses showed that metformin suppressed the ability to form spheroid cells and the expression of stemness markers in HCT116 colorectal cancer cells. …”
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“…Despite their clinical importance, the molecular events that control spheroid attachment and invasion into underlying healthy tissues remain poorly understood. …”
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“…Herein, we report a bioprinting method for recapitulating the TME with a controllable spheroid size. The TME was constructed by printing a blood vessel layer consisting of fibroblasts and endothelial cells in gelatine, alginate, and fibrinogen, followed by seeding multicellular tumour spheroids (MCTSs) of glioblastoma cells (U87 MG) onto the blood vessel layer. …”
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“…Computer simulations of glioma multicellular tumor spheroid (MTS) growth are being carried out for various conditions. …”
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“…We then established a workflow for spheroid manipulation and immune phenotyping. This described workflow conserves media and reagent, facilitates the uninterrupted tracking of spheroid formation under various conditions, and enables 3D-marker analysis by means of 3D epifluorescence deconvolution microscopy. …”
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“…Some of the advantages of functional 3D spheroids include better representation of the in vivo physiology and tumor characteristics when compared to traditional 2D cultures. …”
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“…Relative to their differentiated progeny, tumour spheroid cells were more resistant to the chemotherapeutic irinotecan. …”
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“…All cell lines formed spheroids that could self-renew and be serially re-passaged. …”
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“…In the absence of a spheroid or embryo, migration of H-EnSCs from fertile or RM women was similar. …”
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“…Hypoxia-induced apoptosis under VEGF inhibited conditions was measured by TUNEL assay. Spheroid formation ability was assessed using a 3-D spheroid cell culture system. …”
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“…Disc colonies were found to have comparable levels of E-cadherin as the spheroid colonies, but showed decreased E-cadherin at the cell-matrix contact sites. …”
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“…The expression levels of stemness genes in spheroid and parental cells have investigated using SYBR Green real-time PCR. …”
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“…S703A cells also had reduced metastatic potential and markedly decreased rates of migration, invasion, spheroid growth, anchorage-dependent and soft agar colony formation. …”
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