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90581por Lyu, Xi, Song, Ai-Lin, Bai, Yin-Liang, Xu, Xiao-Dong, He, Dong-Qiang, Zhang, You-Cheng“…Cell apoptosis was analyzed by flow cytometry. Hoechst 33258 staining was used to visualize morphological changes. …”
Publicado 2019
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90582por Xing, Yan, Lin, Nancy U., Maurer, Matthew A., Chen, Huiqin, Mahvash, Armeen, Sahin, Aysegul, Akcakanat, Argun, Li, Yisheng, Abramson, Vandana, Litton, Jennifer, Chavez-MacGregor, Mariana, Valero, Vicente, Piha-Paul, Sarina A., Hong, David, Do, Kim-Anh, Tarco, Emily, Riall, Dianna, Eterovic, Agda Karina, Wulf, Gerburg M., Cantley, Lewis C., Mills, Gordon B., Doyle, L. Austin, Winer, Eric, Hortobagyi, Gabriel N., Gonzalez-Angulo, Ana Maria, Meric-Bernstam, Funda“…By IHC, there was no significant decrease in median pAKT S473 or Ki-67 staining, but a drop was observed in both responders. …”
Publicado 2019
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90583por Li, Si-Si, Jiang, Wei-Liang, Xiao, Wen-Qin, Li, Kai, Zhang, Ye-Fei, Guo, Xing-Ya, Dai, Yi-Qi, Zhao, Qiu-Yan, Jiang, Ming-Jie, Lu, Zhan-Jun, Wan, Rong“…Apoptosis, mitochondrial membrane potential (MMP), reactive oxygen species (ROS) levels, and cell cycle profile were determined by staining the cells with Annexin-V/7-AAD, JC-1, DCFH-DA, and PI respectively, as well as flow cytometric acquisition. …”
Publicado 2019
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90584por Volarevic, Vladislav, Markovic, Bojana Simovic, Jankovic, Marina Gazdic, Djokovic, Bojana, Jovicic, Nemanja, Harrell, C. Randall, Fellabaum, Crissy, Djonov, Valentin, Arsenijevic, Nebojsa, Lukic, Miodrag L.“…Biochemical, histological analysis, enzyme-linked immunosorbent assay (ELISA), immunohistochemistry, real-time PCR, magnetic cell sorting, flow cytometry and intracellular staining of renal-infiltrated immune cells were used to determine the differences between CDDP-treated WT and Gal-3(-/-) mice. …”
Publicado 2019
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90585“…Skin biopsy was non-specific but showed weak staining for IgM and C3 raising the possibility of vasculitis but was not definitive. …”
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90586por Kirkpatrick, Greg, Jones, Courtney, Fosmire, Susan, Porter, Christopher, DiPaola, Jorge“…Mouse bone marrow was isolated and fractioned into cells committed to the B cell lineage via B220+ and CD43+ staining by flow cytometry and then separated into the following fractions: Fraction A (CD24low, CD19−), Fraction B (CD19+, CD24+, BP1−), and Fraction C (CD19+ CD24+ BP1+). …”
Publicado 2018
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90587por McGarry, Sean D., Hurrell, Sarah L., Ickzkowski, Kenneth, Banerjee, Anjishnu, Jacobsohn, Kenneth, Hall, William, Hohenwalter, Mark, LaViolette, Peter, Kaczmarowski, Amy, Keuter, Tucker, Nevalainen, Marja, See, William“…Whole mount samples at 10 μm thickness were taken, hematoxylin and eosin stained, digitized, and annotated by a board certified pathologist. …”
Publicado 2018
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90588“…Afterwards, slices of the brain are stained with H&E, which serves as the gold-standard of the extent of tumor. …”
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90589por Huaman, Moises, Qualls, Joseph E., Kuhel, David, Jose, Shinsmon, Konaniah, Eddy, Komaravolu, Ravi, Fichtenbaum, Carl J., Deepe, George S., Hui, David Y.“…Mice were euthanized after 16 weeks of WD to examine atherosclerotic lesions in aortic root sections and en face aorta using Oil Red O staining. Plasma cholesterol and triglyceride levels were measured by enzymatic assays and lipoprotein distribution was assessed using fast protein liquid chromatography. …”
Publicado 2019
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90590por Fang, Jiankang, Zhao, Xia, Li, Shuai, Xing, Xingan, Wang, Haitao, Lazarovici, Philip, Zheng, Wenhua“…Cell viability was detected by MTT, FACS, LDH, and Hoechst 33342 staining assays. Mitochondrial membrane potential (ΔΨm) was measured by JC-1 assay. …”
Publicado 2019
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90591“…RESULTS: Biochemical assays and haematoxylin and eosin staining suggested severe liver function injury and liver tissue structure damage in the OJ model group. …”
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90592por Zhang, Dong-Xing, Kang, Yuan-Huan, Zhan, Sheng, Zhao, Ze-Lin, Jin, Sheng-Nan, Chen, Chong, Zhang, Lei, Shen, Jin-Yu, Wang, Chun-Feng, Wang, Gui-Qin, Shan, Xiao-Feng, Qian, Ai-Dong“…B. velezensis isolates showed typical Bacillus characteristics by endospore staining, physiological and biochemical test, enzyme activity analysis (amylase, protease, and lipase), and molecular identification. …”
Publicado 2019
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90593por Sayers, Sophie R., Beavil, Rebecca L., Fine, Nicholas H. F., Huang, Guo C., Choudhary, Pratik, Pacholarz, Kamila J., Barran, Perdita E., Butterworth, Sam, Mills, Charlotte E., Cruickshank, J. Kennedy, Silvestre, Marta P., Poppitt, Sally D., McGill, Anne-Thea, Lavery, Gareth G., Hodson, David J., Caton, Paul W.“…Islet cell number was determined by immunohistochemical staining for insulin, glucagon and somatostatin, with islet apoptosis determined by caspase 3/7 activity. …”
Publicado 2019
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90594por Wang, Lixin, Cao, Ying, Guan, Zhizhong, Luo, Guangheng, Luo, Lei, Yang, Xiushu, Chu, Mingliang“…The histopathologic characteristics were observed by hematoxylin and eosin(HE)staining, and the expression of transforming growth factor-β(1) (TGF-β(1)) and casein kinase-2 interacting protein-1 (CKIP-1) were examined by immunohistochemistry in prostatic urethra and bladder neck wound, respectively. …”
Publicado 2019
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90595por Valenzuela-Vazquez, Lucero, Núñez-Enríquez, Juan Carlos, Sánchez-Herrera, Jacqueline, Jiménez-Hernández, Elva, Martín-Trejo, Jorge Alfonso, Espinoza-Hernández, Laura Eugenia, Medina-Sanson, Aurora, Flores-Villegas, Luz Victoria, Peñaloza-González, José Gabriel, Refugio Torres-Nava, José, Espinosa-Elizondo, Rosa Martha, Amador-Sánchez, Raquel, Santillán-Juárez, Jessica Denisse, Flores-Lujano, Janet, Pérez-Saldívar, María Luisa, García-López, Luis Ramiro, Castañeda-Echevarría, Alejandro, Rodríguez-Leyva, Francisco, Rosas-Vargas, Haydeé, Mata-Rocha, Minerva, Duarte-Rodríguez, David Aldebarán, Sepúlveda-Robles, Omar Alejandro, Mancilla-Herrera, Ismael, Mejía-Aranguré, Juan Manuel, Cruz-Munoz, Mario Ernesto“…NK cell function was evaluated by degranulation assays towards K562 cells and SLAM-associated protein (SAP) expression was measured by intracellular staining. All assays were performed using peripheral blood mononuclear cells from controls and patients. …”
Publicado 2020
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90596por Zeng, Xin, Zhu, Min, Liu, Xiaohong, Chen, Xuanmin, Yuan, Yujia, Li, Lan, Liu, Jingping, Lu, Yanrong, Cheng, Jingqiu, Chen, Younan“…The morphology of pyroptosis was detected by acridine orange and ethidium bromide (AO/EB) staining. The release of IL-1 beta and tumor necrosis factor alpha (TNF-α) was determined by ELISA. …”
Publicado 2020
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90597“…The general performance of the mice during modeling, the gross changes of the colon and the rectum, and the pathological score of HE staining were used to observe tumor growth. The expression of TLR2 was detected by immunohistochemistry, and tumor proliferation was detected by Ki67 labeling. …”
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90598Publicado 2020“…The expression of programmed cell death ligand 1 (PD-L1) in 8 patients was detected. When membranous staining was present in ≥5% of the cells, it was defined as PD-L1 positive. …”
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90599“…Differentiation was confirmed by lineage-specific cell staining and quantified by the relative gene expression of lineage-specific markers. …”
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90600por Wang, Jing, Zhao, Ming, Jia, Peng, Liu, Fang-Fang, Chen, Kun, Meng, Fei-Yang, Hong, Jiang-Hao, Zhang, Ting, Jin, Xiao-Hang, Shi, Juan“…Western blot, immunohistochemical, and immunocytochemical stainings were employed to examine the level and expression feature of ionized calcium-binding adaptor molecule 1 (Iba-1), glial fibrillary acidic protein (GFAP), TRPC6, and phosphorylated p38 kinase. …”
Publicado 2020
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