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90661por Zheng, Huifeng, Liang, Xiaoting, Han, Qian, Shao, Zhuang, Zhang, Yuxiao, Shi, Linli, Hong, Yimei, Li, Weifeng, Mai, Cong, Mo, Qingwen, Fu, Qingling, Ma, Xiaoxue, Lin, Fang, Li, Mimi, Hu, Bei, Li, Xin, Zhang, Yuelin“…The mitochondrial morphology of neonatal mice cardiomyocytes (NMCMs) under serum deprivation and hypoxic (SD/H) conditions was examined by Mitotracker staining. The cellular senescence of NMCMs was determined by senescence-associated-β-galactosidase assay. …”
Publicado 2021
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90662por Jing, Wenyi, Lan, Ting, Qiu, Yan, Peng, Ran, Lu, Yang, Chen, Huijiao, Chen, Min, He, Xin, Chen, Chen, Zhang, Hongying“…Among 10 FRS2-nonamplified ALT/WDL/DDL cases, 5 cases were FRS2-immunonegative, and 5 tumors displayed 1+ staining for this marker. In 64 control cases, none of them exhibited FRS2 amplification. …”
Publicado 2021
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90663por Zou, Mingyue, Zhao, Zhenhua, Zhang, Bingqian, Mao, Haijia, Huang, Yanan, Wang, Cheng“…The correlations between CTP parameters with MVD and VEGF were analysed in 36 lung cancer patients who had extra sections be used for immunohistochemistry staining of CD34 and VEGF. RESULTS: BAP (Mean, Std, Q90) and PI Std of benign lesions were higher than malignant lesions (p < 0.05), and PAP (Q10, Q25), PI (Median, Mean, Q10, Q25, Q50) of malignant lesions were higher than the benign (p < 0.05). …”
Publicado 2021
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90664por Cao, Dongdong, Wu, Shuyuan, Wang, Xiaolu, Li, Ying, Xu, Huiling, Pan, Zemin, Wu, Zhaofu, Yang, Lei, Tan, Xiaohua, Li, Dongmei“…The effect of KSHV infection on cell proliferation was detected by MTT and Ki-67 staining. Cell migration was evaluated by Transwell and wound healing assays. …”
Publicado 2021
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90665por Xiong, Yan, Li, Xin, Liang, Li, Li, Dong, Yan, Limin, Li, Xueying, Di, Jiting, Li, Ting“…All CNB samples were stained by immunohistochemistry (IHC) using antibodies against CK19, galectin-3, HBME-1, and CD56 and detected by next-generation sequencing (NGS) using an OncoAim® thyroid cancer multigene assay kit (Singlera Genomics) that detected 26 genes. …”
Publicado 2021
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90666por Kim, Ji Won, Park, Miso, Kim, Suntae, Lim, Sung Chul, Kim, Hyung Shik, Kang, Keon Wook“…In order to demonstrate the interaction between androgen receptor (AR) and YAP1, co-immunoprecipitation (co-IP), immunofluorescence (IF) staining, chromatin immunoprecipitation (ChIP) were performed in LNCaP cells with and without GV1001 treatment. …”
Publicado 2021
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90667por Mohamed, Nouh Saad, AbdElbagi, Hanadi, Elsadig, Ahad R., Ahmed, Abdalla Elssir, Mohammed, Yassir Osman, Elssir, Lubna Taj, Elnour, Mohammed-Ahmed B., Ali, Yousif, Ali, Mohamed S., Altahir, Omnia, Abubakr, Mustafa, Siddig, Emmanuel Edwar, Ahmed, Ayman, Omer, Rihab Ali“…Malaria diagnosis was made by expert microscopists using Giemsa-stained thick and thin blood films. DNA samples were examined by the semi-nested polymerase chain reaction (PCR). …”
Publicado 2021
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90668por Katz, Ian, O’Brien, Blake, Clark, Simon, Thompson, Curtis T., Schapiro, Brian, Azzi, Anthony, Lilleyman, Alister, Boyle, Terry, Espartero, Lore Jane L., Yamada, Miko, Prow, Tarl W.“…A total of 217 lesions removed and prepared by conventional histologic method and stained with hematoxylin-eosin were reviewed by up to 9 independent pathologists for diagnosis using the MOLEM reporting schema. …”
Publicado 2021
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90669“…Xenograft tumor tissues were collected for hematoxylin and eosin (HE) staining and IHC analysis to evaluate histology and Ki-67. …”
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90670por Wu, Yi, Xu, Ling, Song, Yilin, Zhang, Qing, Qin, Guanghao, Yang, Lanting, Ma, Jinfei, Palme, Christoph, Moore, Jonathan E., Pazo, Emmanuel Eric, He, Wei“…Non-invasive tear break-up time (NITBUT), tear film lipid layer (TFLL), lower tear meniscus height (LTMH), meibomian gland quality (MGQ), meibomian gland expressibility (MGEx), corneal fluorescein staining (CFS), ocular surface disease index (OSDI) and artificial tear usage (ATU) survey were assessed at baseline (BL) and follow-up at 2 (F1) and 4 weeks (F2). …”
Publicado 2021
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90671“…METHODS: Hematoxylin–eosin (HE) and immunohistochemistry (IHC) staining were used to present the clinicopathological characteristics of 10 Chinese G34-DHG patients treated at our institution. …”
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90672por Sun, Wei-Wei, Yan, Xiu-Mei, Qiao, Ai-Jun, Zhang, Yuan-Jiao, Yang, Ling, Huang, Hui-Cong, Shi, Hong-Fei, Yan, Bao-Long“…Then, oxidative stress assay and Oil Red O fat staining were used to determine the functions of Acan-Gal-1 in C. elegans. …”
Publicado 2022
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90673“…Next-generation sequencing showed biallelic inactivation mutation of ARID1A; the predicted inactivating effect of ARID1A deletion was confirmed by immunohistochemical staining. After the surgery, the patient received an alternative combined chemotherapy of doxorubicin and ifosfamide for 5 months. …”
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90674por Dang, Ruozhi, Wang, Mingyang, Li, Xinhui, Wang, Haiyang, Liu, Lanxiang, Wu, Qingyuan, Zhao, Jianting, Ji, Ping, Zhong, Lianmei, Licinio, Julio, Xie, Peng“…Hippocampal and medial prefrontal cortex (mPFC) tissues were collected for Nissl staining, immunofluorescence, targeted energy metabolomics analysis, enzyme-linked immunosorbent assay (ELISA), measurement of MDA, SOD, GSH, GSH-PX, T-AOC and transmission electron microscopy (TEM). …”
Publicado 2022
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90675por dos Santos, Jôiciglecia Pereira, de Souza, Valesca Ferreira Machado, Santos, Marcos Wilker da Conceição, dos Santos, Juliany Nunes, dos Santos, Natilene Silva, de Oliveira, Angélica Prado, Rodrigues, Valquíria Tatiele da Silva, Carneiro, Ianei Oliveira, Vieira, Layze Cilmara Alves da Silva“…MATERIALS AND METHODS: The biological samples (21) were inoculated to brain heart infusion broth, blood agar, and MacConkey agar and incubated for 24-72 h at 37°C. Gram staining and identification through biochemical tests and matrix-associated laser desorption/ionization time-of-flight mass spectrometry were conducted. …”
Publicado 2021
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90676por Guchhait, Kartik Chandra, Manna, Tuhin, Barai, Manas, Karmakar, Monalisha, Nandi, Sourav Kumar, Jana, Debarati, Dey, Aditi, Panda, Suman, Raul, Priyanka, Patra, Anuttam, Bhattacharya, Rittwika, Chatterjee, Subhrangsu, Panda, Amiya Kumar, Ghosh, Chandradipa“…Bacterial cells were studied by fluorescence microscopy using acridine orange/ethidium bromide as the staining agents. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values were evaluated to observe the antibacterial activities. …”
Publicado 2022
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90677por Termer, Michael, Jaeger, Anita, Carola, Christophe, Salazar, Andrew, Keck, Cornelia M., Kolmar, Harald, von Hagen, Joerg“…Immunohistochemistry staining of ferritin on human skin explants further showed that the treatment with MeO-MBM reduced the ferritin expression. …”
Publicado 2021
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90678por Zhang, Leyi, Lu, Chenglong, Kang, Li, Li, Yingji, Tang, Wenjing, Zhao, Dengfa, Yu, Shengyuan, Liu, Ruozhuo“…We detected the expression profile of glial fibrillary acidic protein (GFAP) in the TNC through immunofluorescence staining and western blot assay. We also investigated the variation with time of the transcriptional levels of GFAP and ionized calcium binding adapter molecule 1 (Iba1) through RNAscope in situ hybridization analysis. …”
Publicado 2022
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90679por Palacios-Saucedo, Gerardo del Carmen, Rivera-Morales, Lydia Guadalupe, Vázquez-Guillén, José Manuel, Caballero-Trejo, Amilcar, Mellado-García, Melissa Carolina, Flores-Flores, Aldo Sebastián, González-Navarro, José Alfredo, Herrera-Rivera, Celia Geovana, Osuna-Rosales, Luis Ernesto, Hernández-González, Julio Antonio, Vázquez-Juárez, Ricardo, Barrón-Enríquez, Carolina, Valladares-Trujillo, Ramón, Treviño-Baez, Joaquín Dario, Alonso-Téllez, César Alejandro, Ramírez-Calvillo, Luis Daniel, Cerda-Flores, Ricardo Martín, Ortiz-López, Rocío, Rivera-Alvarado, Miguel Ángel, Solórzano-Santos, Fortino, Castro-Garza, Jorge, Rodríguez-Padilla, Cristina“…After overnight incubation, suggestive colonies with or without hemolysis were analyzed to confirm GBS identification by Gram staining, catalase test, hippurate hydrolysis, CAMP test, and incubation in a chromogenic medium. …”
Publicado 2022
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90680por Duan, Jiali, Gao, Jing, Liu, Qiuhong, Sun, Mengfei, Liu, Yang, Tan, Yingshuai, Xing, Lihua“…RESULTS: Metagenomic next-generation sequencing (mNGS) showed a satisfying diagnostic performance of 100% positive of detecting P. jirovecii from bronchoalveolar lavage (BAL) specimens in forty-six patients with non-HIV PCP, compared to only 15.2% for Gomori Methenamine silver (GMS) staining and 84.8% for Serum 1,3-beta-D-glucan (BDG). …”
Publicado 2022
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