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90701por Sarenje, Kelvin L., Ngalamika, Owen, Maimbolwa, Margaret C., Siame, Amon, Munsaka, Sody M., Kwenda, Geoffrey“…Identification of N. gonorrhoeae isolates was achieved by Gram stain, oxidase, nitrocefin disk, BactiCard Neisseria, and Viteck® Compact. …”
Publicado 2022
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90702por Pirlog, Cristina-Florina, Cotan, Horia Teodor, Parosanu, Andreea, Orlov Slavu, Cristina, Popa, Ana Maria, Iaciu, Cristian, Olaru, Mihaela, Oprita, Alexandru Vlad, Nita, Irina, Nitipir, Cornelia“…PD-L1 expression was determined by immunohistochemical staining. Univariate and multivariate survival analyses were conducted to test their prognostic value. …”
Publicado 2022
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90703por Luo, Qianqian, Tang, Yu, Jiang, Zhonglin, Bao, Hongchu, Fu, Qiang, Zhang, Hongqin“…Then, using ELISA, HE staining, TUNEL apoptosis test kit, immunohistochemistry and western blot, researchers examined hormonal levels, ovarian morphology, TICs apoptosis, NR4A1 and Cyp17a1 in response to cisplatin treatment and hUCMSCs. …”
Publicado 2022
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90704por Huang, Qiubin, Liu, Huiping, Zhang, Juanmei, Wang, Shaowei, Liu, Fengying, Li, Chengdie, Wang, Gang“…METHODS: Mutant gene strains and fluorescently tagged strains were constructed by homologous recombination, and amylase protein was expressed in the prokaryotic Escherichia coli BL21(DE3) expression system. The iodine staining method was used to measure the activity of amylase proteins. …”
Publicado 2022
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90705“…Based on the results of diagnostic investigations which confirmed the possibility of the tumor Ewing sarcoma of esophagus, which was biopsy and immune histochemical stain proven the patient was qualified for surgical treatment. …”
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90706por ZHANG, Mengting, ZHANG, Yulu, WANG, Haojiang, LI, Ning, LI, Bo, XIAO, Hong, BIAN, Wei, CAI, Zongwei“…Mass spectrometry imaging (MSI) is a useful technique for acquiring molecular information about biological tissues, including qualitative, quantitative, and spatial distribution information, because it is based on the ion mass-to-charge ratio of the biomolecules and avoids the need for their labeling and staining. MSI can also acquire molecular information on drugs and their metabolites, as well as that on molecules related to endogenous metabolism, such as lipids, peptides, and proteins. …”
Publicado 2021
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90707por CHEN, Guohong, GUO, Zehua, CAO, Yiren, FAN, Liuyin, LIU, Weiwen, MA, Yixin, CAO, Chengxi, ZHANG, Qiang“…This method can be used in beads/spots-based immunoassay in gel, immuno-electrophoresis, and fluorescent staining of protein/nucleic acid bands in gel electrophoresis.…”
Publicado 2022
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90708por Zafar, Naheed, Uzair, Bushra, Menaa, Farid, Khan, Barkat Ali, Niazi, Muhammad Bilal Khan, Alaryani, Fatima S., Majrashi, Kamlah Ali, Sajjad, Shamaila“…Further, biological studies were carried out both ex vivo (i.e., by disk diffusion method (DDM), fluorescence-activated single cell sorting (FACS), MTT assay) and in vivo (i.e., antibacterial activity in a rabbit model, colony-forming unit (CFU) on blood agar, histopathological analysis using H&E staining). Results: The encapsulation efficiency (EE) and the loading capacity (LC) of the NDDS were as high as 94% ± 1.26 and 57% ± 3.5, respectively. …”
Publicado 2022
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90709“…First, a NASH mouse model was established by feeding a methionine choline-deficient (MCD) diet. Hematoxylin-eosin staining of liver tissues showed successful modeling of inflammatory injury in the mouse liver. …”
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90710“…LYVE-1, VEGF-C/D, and VEGFR-3 levels in recurrence tumors and/or lung tissues were determined by immunohistochemistry staining. RESULTS: The mRNA, as well as protein expression of GSK-3β were up-regulated and the mRNA expression of β-catenin was down-regulated after PBMF treatment. …”
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90711por Chaudhary, Pankaj, Basnet, Samridhi, Chaulagain, Manisha, Khadgi, Anu, B.K, Samana“…Preliminary identification of the Candida was done on the basis of colony characteristics, gram staining, and germ tube test. Further for the speciation of Candida species, HiCrome™ Candida Differential Agar (HImedia, India) was applied where on the basis of coloration and colony morphology speciation was done. …”
Publicado 2022
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90712por Sharma, Arunima, Singh, Shreya, Dogra, Sunil, Narang, Tarun, Saikia, Biman, Saikia, Uma Nahar, Chakrabarti, Arunaloke, Kaur, Harsimran, Ghosh, Anup, Rudramurthy, Shivaprakash M.“…The viability and Cell-cycle analysis of HaCaT cells in the presence and absence of clobetasol propionate (0.05% w/w) was performed by MTT assay and Propidium Iodide (PI) staining via flow cytometry, respectively. Growth kinetics of dermatophytes was performed for 96 h in presence and absence of corticosteroid. …”
Publicado 2022
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90713por Romald, Packia Nancy, Kindo, Anupma Jyoti, Veereraghavan, Mahalakshmi, Umadevi, U.“…Samples were cultured and Phenotypic Identification was done based on colony growth characteristics, gram staining, urease test, and catalase test, bile esculin with tween 60 hydrolysis and tween assimilation. …”
Publicado 2022
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90714por Perween, Naz, Nandwani, Sumi, Verma, Nikhil, Rai, Sumit, Prakash, M.R. Shiva, Saxena, Krati, Dwivedi, Niharika“…Initially the samples, with probable yeast were inoculated on Saboraud Dextrose agar (SDA) with chloramphenicol incubated at 37(0)C. Gram stain was done from the culture growth look for yeast cells. …”
Publicado 2022
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90715“…Lung tissues were collected on day 21, and hematoxylin–eosin (HE) and Masson staining were performed to assess morphological changes and collagen deposition in the lungs. …”
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90716por Tang, Haiying, Li, Jiahui, Zinker, Bradley, Boehm, Stephanie, Mauer, Amy, Rex‐Rabe, Sandra, Glaser, Kevin J., Fronheiser, Matthew, Bradstreet, Thomas, Nakao, Yasuhiko, Petrone, Thomas, Pena, Adrienne, Villano, MacKenzie, Chow, Patrick, Malhi, Harmeet, Charles, Edgar D., Hayes, Wendy, Ehman, Richard L., Du, Shuyan, Yin, Meng“…Fibrosis and steatosis were measured histologically after picrosirius red and H&E staining. Alkaline phosphatase, alanine transaminase, bile acids, and triglycerides (TGs) were measured. …”
Publicado 2022
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90717“…Case 1: Mr S.O, 26 years old man, presenting a dysmorphic syndrome associating short stature, facial dysmorphism, dental staining, gingival retractions, recurrent bronchial infections, mitral valve disease and joint deformities with a tunnel syndrome. …”
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90718“…The expression levels of YKL-40, Wnt4, β-catenin and TGF-β (1) mRNA in renal tissue were detected by fluorescence quantitative PCR. Hematoxylin-eosin staining was used to observe the changes in renal pathological injury in each group; GC-MS detected the changes of short chain fatty acid content. …”
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90719“…The CIBERSORT algorithm showed increased M0 and M1, decreased M2 macrophage infiltration in NAFLD with comparison to healthy obese group; (4) After 12 weeks of HFD-feeding, the obesity mice had significantly higher serum TSH and In IHC-stained liver sections of obesity group, the intensity of SPP1 had a significantly increased, while TRβ reduced; (5) In vitro studies have shown SPP1 aggravated lipid deposition in hepatic cells dependent on down-regulating the expression of TRβ and TSH acts to promote secretion of SPP1 in M1 macrophage cells. …”
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90720por Lazcano, Rossana, Barreto, Carmelia M., Salazar, Ruth, Carapeto, Fernando, Traweek, Raymond S., Leung, Cheuk H., Gite, Swati, Mehta, Jay, Ingram, Davis R., Wani, Khalida M., Vu, Kim-Anh T., Parra, Edwin R., Lu, Wei, Zhou, Jianling, Witt, Russell G., Cope, Brandon, Thirasastr, Prapassorn, Lin, Heather Y., Scally, Christopher P., Conley, Anthony P., Ratan, Ravin, Livingston, J. Andrew, Zarzour, Alexandra M., Ludwig, Joseph, Araujo, Dejka, Ravi, Vinod, Patel, Shreyaskumar, Benjamin, Robert, Wargo, Jennifer, Wistuba, Ignacio I., Somaiah, Neeta, Roland, Christina L., Keung, Emily Z., Solis, Luisa, Wang, Wei-Lien, Lazar, Alexander J., Nassif, Elise F.“…MATERIAL AND METHODS: Surgically resected UPS samples were stained by immunohistochemistry (IHC) for the following: tumor-associated immune cells (CD3, CD8, CD163, CD20), immune checkpoints (stimulatory: OX40, ICOS; inhibitory: PD-L1, LAG3, IDO1, PD1), and the adenosine pathway (CD73, CD39). …”
Publicado 2022
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