“…Triple immunofluorescence staining of decidua tissues identified expression of ER46 in the cell membrane of CD56-positive uNK cells which were otherwise ER66-negative. …”
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“…Biochemical methods included anti-isoD7-Aβ monoclonal antibody characterization by surface plasmon resonance, immunohistochemical staining of human and transgenic mouse brain, and the development and application of isoD7-Aβ ELISA as well as different non-modified Aβ ELISA. …”
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“…The levels of NO, ANG II, and ET-1 in the serum were measured, and pathological changes in the heart were examined by H&E staining. The main active components of LFG, their corresponding targets, and hypertension associated pathways were discerned through network pharmacology analysis based on the Traditional Chinese Medicine Systems Pharmacology (TCMSP), Traditional Chinese Medicine Integrated Database (TCMID), and the Bioinformatics Analysis Tool for Molecular Mechanism of Traditional Chinese Medicine (BATMAN-TCM). …”
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“…Both patients had bone marrow specimens stained for SARS-CoV-2 by immunohistochemistry that were negative. …”
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“…Orchiectomy specimen sections were stained with Mayer’s hematoxylin and eosin and histologically analyzed to assess the Johnsen score and the ratio of most advanced germ cell types in at least 50 seminiferous tubular cross-sections. …”
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“…In addition, at 10 days after cell transplantation, old MDSCs having undergone double transduction regenerated bone more rapidly as showed by Alcian blue and Von Kossa staining. Western blot assays demonstrated that old MDSCs after retro-BMP4/GFP double transduction have significantly lower P18 expression levels when compared to young BMP4-transduced MDSCs. …”
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“…Focus score nearly reaching 50 cells. Plasma cell staining ruled out IgG4 related disease. Ophthalmic assessment ruled out eye involvement by the appearance of her eyes. …”
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“…RNA-seq, real-time PCR, immunofluorescence staining and Western blot were used in the above observation. …”
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“…The monoclonal antibody 2.4G2, which detects both FcγRII and RIII, stained macrophages from the synovial lining of DBA/1 mice more intensely than those from C57BL/6 mice. …”
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“…After 3 weeks, rats were euthanized, and the menisci were histologically evaluated by hematoxylin and eosin (H&E) staining. The histologic assessment and scoring of meniscus tissue wound healing were done by one blinded and one unblinded observer, each using selected wound healing parameters as described in Table 1, and the results were averaged. …”
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“…The length of needle was more than 11.9 mm and remained roughly consistent. HE staining was performed after puncture biopsy. The positive rate of biopsy was observed. …”
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“…STUDY DESIGN, SIZE, DURATION: Blastocysts from old (34–39 weeks) or young (8–9 weeks) C57BL/6 females mated with young CBA males (13–15 weeks) were either subjected to differential cell staining (inner cell mass and trophectoderm) or underwent embryo transfer (ET) into young MF1 surrogates (8–9 weeks) to produce young (Young-ET, 9 litters) and old (Old-ET, 10 litters) embryo-derived offspring. …”
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“…Furthermore, we investigated lineage-specific staining and gene expression after MSC trilineage differentiation. …”
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“…The mesh-tissue complex was removed from each rabbit and processed for histological staining as well as immunolabeling of immune cells, such as macrophages. …”
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“…Cell viability was measured via MTT assay (Vybrant(®) MTT Cell Proliferation Assay, Invitrogen) and by live/dead staining (LIVE/DEAD(®) Cell Imaging Kit, Invitrogen). …”
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“…Malignancy could be excluded, and the absence of characteristic histochemical staining patterns favoured sarcoid to other differential diagnoses. …”
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“…After treatment, cells were collected to either assess the expression of mRNAs and miR-29b by real-time PCR or to determine the expression of tight junction-associated proteins and HIF-1α nuclear accumulation by Western blot and/or immunofluorescent staining; the conditioned media were collected to evaluate the MMP-2 and MMP-9 activities by gelatin zymography assay. …”
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“…We analysed the magnitude and breadth of the SARS-CoV-2-specific memory T-cell responses using the interferon γ (IFNγ) enzyme-linked immune absorbent spot (ELISpot) assay and intracellular cytokine staining (ICS) assay. The antibody response and T-cell response (ie, IFN-γ, interleukin-2 [IL-2], and tumour necrosis factor α [TNFα]) were analysed by age and disease severity. …”
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“…All specimens were prepared for the following outcomes: (1) measurements of mitochondrial metabolism (SOD activity); (2) analysis of mitochondrial morphology (TEM); (3) membrane polarity assay using mito-trackergreen/Tetramethylrhodamine methyl ester staining); (4) cell viability (CCK8). STATISTICAL ANALYSIS: One-way ANOVA with Tukey’s test were used. …”
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“…Patients with anti PM/Scl 75/100 show a nucleolar pattern on ANA staining as in our patient and usually show better prognosis in terms of ILD. …”
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