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  1. 1461
  2. 1462
    “…Using an open-source computer-based imaging software, an algorithm was developed for quantification of staining based on a signal-to-background ratio. Next, two blinded observers evaluated images and assigned a semi-quantitative visual score based on surface staining (0—no blue stain, 1−1–50% stained, and 2−51–100% stained) and those results were compared to the established quantitative standard. …”
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  3. 1463
    “…Its applicability is limited in some cases due to the additional risks associated with prolonged surgery, largely from the time-consuming staining procedure. Our work uses a measurable property of bulk tissue to bypass the staining process: as tumour cells proliferate, they influence the surrounding extra-cellular matrix, and the resulting change in elastic modulus provides a signature of the underlying pathology. …”
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  4. 1464
  5. 1465
    “…This plasma cell percentage is often determined by visual estimation of CD138-stained bone marrow biopsies and clot sections. While not necessarily inaccurate, estimates are by definition imprecise. …”
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  6. 1466
    “…RESULTS: LU and GR revealed the highest differences in whiteness index after coffee staining (P < 0.001). GR revealed lower translucency parameter differences after staining with coffee than LU (P = 0.007). …”
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  7. 1467
  8. 1468
  9. 1469
    “…Intramuscular neural arborization was visualized by Sihler’s staining. The supraspinatus muscle was divided into four portions named A, B, C, and D. …”
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  10. 1470
  11. 1471
  12. 1472
  13. 1473
    “…Next, a user-defined tolerance for error is applied to the negative control slides to set a NRMB threshold distinguishing stained from unstained tissue and this threshold is applied to calculate the fraction of stained tissue pixels on each test slide. …”
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  14. 1474
  15. 1475
    “…METHODS: Paraformaldehyde plus saponin or the eBioscience Foxp3/Transcription Factor Staining Buffer Set were used to evaluate the non-specific intracellular retention of phycoerythrin-containing antibody conjugates by ASCs. …”
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  16. 1476
    “…Here we introduce a new seed-tracking method using safranine to stain seeds/fruits by immersing in (ex situ) or spraying with (in situ) staining solution. …”
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  17. 1477
    “…Bar-shaped and rectangle-shaped specimens of the 5Y-PSZ were prepared and divided into six groups, according to finishing procedure: GC (control), GS (staining), GG (glazing), GSG (staining and glazing), GP (polishing), GSP (staining and polishing). …”
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  18. 1478
    “…However, traditional manual imaging of sections stained with immunohistochemistry is laborious, time-consuming, and often assesses fields of view rather than the whole tissue section. …”
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  19. 1479
    “…SLNB was performed by ICG combined with methylene blue staining under the guidance of the fluorescent tracer navigation system FLI-10B. …”
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  20. 1480
    “…The use of the available drug as an inter-ESPT fluorescent probe is simple and accurate, providing a good method for Fe(3+) ion sensing and protein staining.…”
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