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781por Islamuddin, Mohammad, Chouhan, Garima, Want, Muzamil Y., Tyagi, Maujiram, Abdin, Malik Z., Sahal, Dinkar, Afrin, Farhat“…The effect was mediated through programmed cell death as confirmed by externalization of phosphatidylserine, DNA nicking by TdT-mediated dUTP nick-end labeling assay, dyskinetoplastidy, cell cycle arrest at sub-G(0)–G(1) phase, loss of mitochondrial membrane potential and reactive oxygen species generation in promastigotes and nitric oxide generation in ex vivo model. …”
Publicado 2014
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782por Yan, Lifeng, Guo, Wenhui, Wu, Shengmin, Liu, Jining, Zhang, Shenghu, Shi, Lili, Ji, Guixiang, Gu, Aihua“…Sperm DNA fragmentation was detected using the Tdt-mediated dUTP nick-end labeling assay, and the level of 8-hydroxydeoxyguanosine (8-OHdG) in sperm DNA was measured using immunofluorescence. …”
Publicado 2014
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783por Wang, Guojin, Wang, Yuanguo, Zhang, Peng, Chen, Yuan, Liu, Yimei, Guo, Feng, Zhang, Hui“…Immunocytochemistry indicated that the cell line positive expression of CK7, CK8/18, CK19, CK-pan, CD24, BCL-2, P63, Vimentin, epithelial membrane antigen and epidermal growth factor receptor, lymphocyte related antigen CD99, and TdT were negatively expressed. CONCLUSIONS: The newly established thymic epithelial tumor cell line from Chinese patients provides a model in the study of thymoma and molecularly targeted therapies.…”
Publicado 2015
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784por Song, Kexin, Zhang, Mingzi, Hu, Jianqiang, Liu, Yunqi, Liu, Yifang, Wang, Youbin, Ma, Xuemei“…On the 3rd postoperative day, a laser Doppler flowmeter was used to measure flap blood supply, and hematoxylin and eosin (H&E) staining was used to observe morphological changes. TdT-mediated dUTP-X nick end labeling (TUNEL) staining was also used to observe early apoptosis and is presented as the percentage of TUNEL-positive cells. …”
Publicado 2015
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785“…The serum levels of malondialdehyde (MDA) and cardiac troponin I (cTnI) and cardiomyocyte apoptosis were detected by enzyme linked immunosorbent assay and TdT-mediated dUTP nick end labeling assays. The mRNA and protein level of calpain-2 were measured by reverse transcriptase polymerase chain reaction and Western blot. …”
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786por Yang, Xin, Zhu, Tian-Yue, Wen, Li-Cheng, Cao, Yong-Ping, Liu, Chao, Cui, Yun-Peng, Meng, Zhi-Chao, Liu, Heng“…We used toluidine blue staining, Mankin scores, and TdT-mediated dUTP-biotin nick end labeling staining to evaluate the therapeutic effect of the injections. …”
Publicado 2015
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787por Volckmar, Anna-Lena, Han, Chung Ting, Pütter, Carolin, Haas, Stefan, Vogel, Carla I. G., Knoll, Nadja, Struve, Christoph, Göbel, Maria, Haas, Katharina, Herrfurth, Nikolas, Jarick, Ivonne, Grallert, Harald, Schürmann, Annette, Al-Hasani, Hadi, Hebebrand, Johannes, Sauer, Sascha, Hinney, Anke“…For SNP Arg695Cys (rs58983546) in TBC1D1 we detected nominal association with obesity (p(TDT) = 0.03 in 705 trios). Eleven of the variants were rare, thus were only detected heterozygously in up to ten individual(s) of the complete screening sample of 372 individuals. …”
Publicado 2016
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788“…Terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labeling (TUNEL) staining, immunohistochemistry staining, and Western blot assay were used to investigate the signal pathway underlying the expression of bFGF in DCM treatment. …”
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789por Chang, Jiwon, Choi, June, Rah, Yoon Chan, Yoo, Myung Hoon, Oh, Kyoung Ho, Im, Gi Jung, Lee, Seung Hoon, Kwon, Soon Young, Park, Hae-Chul, Chae, Sung Won, Jung, Hak Hyun“…Apoptosis and hair cell damage of neuromasts were evaluated using the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick end labeling (TUNEL) assay and 2-[4-(dimethylamino) styryl]-N-ethylpyridinium iodide (DASPEI) assay, respectively. …”
Publicado 2016
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790por Zhang, Li-Da, Ma, Li, Zhang, Li, Dai, Jian-Guo, Chang, Li-Gong, Huang, Pei-Lin, Tian, Xiao-Qiang“…Morris water maze tests were used to assess cognitive, and memory capacities of rats; TdT-mediated dUTP Nick-End Labeling staining and Western blotting were used to analyze apoptosis and NF-κB pathway-related proteins in hippocampus tissues. …”
Publicado 2015
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791“…We established a mouse neonatal myocardial infarction model by visually confirmed ligation of the LAD using microsurgery. TdT-mediated dUTP nick-end labeling (TUNEL) proved reproducible massive myocardial infarctions in a defined region of the apex and anterior wall of neonatal and 7-day-old mice. …”
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792por Thiébaut, Raphaële, Esmiol, Sophie, Lecine, Patrick, Mahfouz, Batoul, Hermant, Aurelie, Nicoletti, Cendrine, Parnis, Stephane, Perroy, Julie, Borg, Jean-Paul, Pascoe, Leigh, Hugot, Jean-Pierre, Ollendorff, Vincent“…Furthermore, to determine whether these NOD2 protein partners could be encoded by IBD susceptibility genes, a transmission disequilibrium test (TDT) was performed on 101 single nucleotide polymorphisms (SNPs) and the main corresponding haplotypes in genes coding for 15 NIPs using a set of 343 IBD families with 556 patients. …”
Publicado 2016
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793“…Terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labeling assay showed a decreased apoptotic index in each group, with a significant decrease in the SB20358 + electroacupuncture group. …”
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794por Folk, Alexandra, Balta, Cornel, Herman, Hildegard, Ivan, Alexandra, Boldura, Oana Maria, Paiusan, Lucian, Ardelean, Aurel, Hermenean, Anca“…Amphotericin B–flucytosine cotreatment increased significantly the number of terminal deoxy-nucleotidyl transferase (TdT)-mediated dUTP nick end-labeling positive nuclei. …”
Publicado 2017
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795por Zhao, Hongjing, He, Ying, Li, Siwen, Sun, Xiao, Wang, Yu, Shao, Yizhi, Hou, Zhijun, Xing, Mingwei“…We found that (1) Oxidative damage and inflammatory response were confirmed in the immune organs of chickens fed on As(2)O(3) diet. (2) Subchronic arsenism induced typical apoptotic changes in ultrastructure. (3) TdT-mediated dUTP Nick-End Labeling (TUNEL) showed that the number of apoptotic cells significantly increased under subchronic arsenism. (4) As(2)O(3)-induced apoptosis of immune organs involved in mitochondrial pathway (decrease of B-cell lymphoma-2 (Bcl-2) and increase of protein 53 (p53), Bcl-2 Associated X Protein (Bax), caspase-9, caspase-3) and death receptor pathway (increase of factor associated suicide (Fas) and caspase-8). …”
Publicado 2017
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796“…The effect of KU60019 in the proliferation and apoptosis of primary cultured rat PA-SMCs was measured by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) and TdT-mediated dUTP nick-end labeling (TUNEL), respectively. …”
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797por Talib, Wamidh H.“…Terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) colorimetric assay and caspase-3 activity assays were used to detect apoptosis. …”
Publicado 2017
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798por Kim, Ju-Ha, Kwon, Hee Young, Ryu, Dong Hoon, Nam, Min-Ho, Shim, Bum Sang, Kim, Jin Han, Lee, Jae Yeol, Kim, Sung-Hoon“…Also, apoptotic bodies and terminal deoxynucleotidyl transferase (TdT) dUTP Nick End Labeling (TUNEL) positive cells were observed in BK10007S treated HepG2 and SK-Hep1 cells by 4',6-diamidino-2-phenylinodole (DAPI) staining and TUNEL assay, respectively. …”
Publicado 2017
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799“…Mice were sacrificed 14 days after TBI, and then brain sections were stained by terminal deoxyribonucleotidyl transferase (TDT)-mediated dUTP-digoxigenin nick end labeling staining to assess brain neuronal apoptosis. …”
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800por Chen, Yi-Xuan, Zhu, Dao-Yu, Yin, Jun-Hui, Yin, Wen-Jing, Zhang, Yue-Lei, Ding, Hao, Yu, Xiao-Wei, Mei, Jiong, Gao, You-Shui, Zhang, Chang-Qing“…Hematoxylin & eosin (H&E) staining combined with TdT-mediated dUTP nick end labeling (TUNEL), cleaved caspase-3 immunohistochemical staining, and micro-CT images revealed substantial ONFH in the alcohol-administered rats, whereas significantly less osteonecrosis developed in the rats injected with PFTα. …”
Publicado 2017
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