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981por Yuan, Tao, Yang, Ni, Bi, Wei, Zhang, Jinwen, Li, Xueyan, Shi, Long, Liu, Yang, Gao, Xiang“…Apoptotic parameters were detected by the TdT-mediated dUTP Nick-End Labeling method. To what extent and levels that antiapoptotic and proapoptotic proteins were expressed could be sensitively revealed by immunohistochemistry assay. …”
Publicado 2021
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982“…MitoTracker Green FM staining and an ALP kit were employed for detection of mitophagy. TdT-mediated dUTP-biotin nick-end labeling assay was used to reveal cell apoptosis. …”
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983por Li, Lu, Liu, Xiaoxi, Li, Shasha, Wang, Qingyan, Wang, Hongru, Xu, Menglu, An, Yanxin“…Abbreviation: Ac-p65: acetylated p65; Ac-foxo 1: acetylated forkhead box O1; AKI: acute kidney injury; BUN: blood urea nitrogen; CAT: catalase; DHE: dihydroethidium; GPx: glutathione peroxidase; GSH: reduced glutathione; IL-1β: Interleukin-1 beta; IL-6: Interleukin-6; KIM-1: kidney injury molecule 1; MDA: malondialdehyde; SCr: serum creatinine; SIRT1: silent information regulator 1; SOD: superoxide dismutase; THC: tetrahydrocurcumin; TNF-α: tumor necrosis factor-alpha; TUNEL: TdT-mediated dUTP Nick-End Labeling; UAlb/Cr: urine micro albumin/creatinine.…”
Publicado 2021
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984“…BACKGROUND: Iron overload is a major problem in patients with transfusion-dependent beta-thalassemia (TDT). Reports on the correlation between iron overload and endocrine function with growth retardation in such a population in Indonesia have not been established. …”
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985“…The primary measured outcomes included the neural functional deficit score (NFS), infarct volume (IV), brain water content, cell viability, apoptotic cells, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick end labeling (TUNEL)-positive cells, B-cell lymphoma-2 (Bcl-2) level and tumor necrosis factor-α (TNF-α) level. …”
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986por He, Xiang, Zhang, Lei, Hu, Lingjuan, Liu, Shengbin, Xiong, Anying, Wang, Junyi, Xiong, Ying, Li, Guoping“…RNA-seq and gene set enrichment analysis (GSEA) was performed to identify the key pathway. TdT mediated dUTP Nick End Labeling (TUNEL) assay, real-time qPCR, Western blot, immunofluorescence and lentivirus transfection were applied for mechanism discovery. …”
Publicado 2021
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987“…According to the results of ORR and CRR, transfusion-dependent thalassemia (TDT) patients showed remarkable efficacy of thalidomide, 83 and 52% respectively. …”
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988por Cheng, Zenghui, Wang, Yixue, Yuan, Min, Liang, Jianxiao, Feng, Yanling, Shi, Yuxin, Zhang, Zhiyong, Shan, Fei“…The parameters (BF, PF and PI of tumor; PF of GGO) of the complete and incomplete RFA groups were compared based on nicotinamide adenine dinucleotide hydrogen (NADH) and TdT-mediated dUTP nick-end labeling (TUNEL) staining and were correlated with the microvascular density (MVD). …”
Publicado 2022
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989por Long, Linzi, Zhang, Xiuli, Wen, Ying, Li, Jiapeng, Wei, Lihui, Cheng, Ying, Liu, Huixin, Chu, Jianfeng, Fang, Yi, Xie, Qiurong, Shen, Aling, Peng, Jun“…Consistently, terminal deoxynucleotidyl transferase (TdT) dUTP nick-end labeling (TUNEL) staining and Annexin V staining revealed that QDG treatment significantly attenuated Ang II-induced cell apoptosis in renal tissues and cultured renal tubular epithelial cell lines (NRK-52E). …”
Publicado 2022
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990por Dong, Wen, Chen, Qingfang, Zhao, Shunying, Wen, Shaohong, Chen, Wentao, Ye, Weizhen, Gong, Ting, Jiang, Mingyu, Liu, Xiangrong“…Cell death after dMCAO was confirmed with terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay. …”
Publicado 2022
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991por Zhou, Peng, Gao, Ge, Zhao, Chun-chun, Li, Jing-ya, Peng, Jian-fei, Wang, Shu-shu, Song, Rui, Shi, Hui, Wang, Liang“…Hematoxylin-eosin (HE) and Masson staining were used to evaluate histological changes, and cardiomyocyte apoptosis was identified using TdT-mediated dUTP nick-end labelling (TUNEL). Enzymatic indexes were determined. mRNA and protein expressions were analysed through RT-qPCR and Western blotting. …”
Publicado 2022
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992por Du, Longlong, Li, Xuefeng, Gao, Qimeng, Yuan, Puwei, Sun, Yindi, Chen, Yingpu, Huang, Bo, Deng, Yu, Wang, Baohui“…Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was applied to verify the expression of NR2F1-AS1 and miR-145-5p in nucleus pulposus (NP) tissues from IVDD patients or NP cells dealt with IL-1β or TNF-α. Flow cytometry or the TdT-mediated dUTP nick end labeling (TUNEL) assay was performed to validate the apoptosis of NP cells with selective regulation of NR2F1-AS1 and miR-145-5p. …”
Publicado 2022
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993“…METHODS: After ALK5 was silenced in SAH mice, neurological function was evaluated, neuron apoptosis was detected by TdT-mediated dUTP-biotin nick end labeling with NeuN staining, and expression of serum inflammatory factors (interleukin-6, interleukin-1β, and tumor necrosis factor-α) was determined by enzyme-linked immunosorbent assay. …”
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994por Lian, Huifang, Fang, XiaoLong, Li, Qingyu, Liu, Shuang, Wei, Qiuhong, Hua, Xia, Li, Wenguang, Liao, Chunyang, Yuan, Xiaoyong“…Gene expression was assessed using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blot analysis or immunofluorescence staining was performed to evaluate protein expression. TdT-mediated dUTP nick end labeling (TUNEL) staining was performed to examine the pyroptotic cell death. …”
Publicado 2022
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995“…The expressions of the vascular endothelial growth factor receptor-2 (VEGFR-2), platelet endothelial cell adhesion molecule-1 (CD31), proliferating cell nuclear antigen (Ki-67), and histone H2AX family member (γ-H2AX) were determined using immunohistochemistry (IHC), the extent of apoptosis was determined using terminal deoxynucleotidyl transferase (TdT)-mediated (dUTP) nick-end labeling (TUNEL), and tumor metabolism was determined using micro18F-fluorodexyglucose positron emission tomography/computed tomography. …”
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996por Zhang, Peixu, Zhang, Xiaobo, Xiong, Peiyu, Zhong, Chun, Zhou, Zhen, Jia, Bo, Liu, Xinglong“…Hematoxylin-eosin (HE) staining was used to observe the pathological change of colon in UC rats after treating RBP, and terminal deoxynucleotidyl-transferase (TdT)-mediated dUTP Nick end labeling (TUNEL) staining was used to detect apoptosis after RBP treatment. …”
Publicado 2022
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997“…The effect of EsA and/or lipopolysaccharide (LPS) on the viability, proliferation, and apoptosis of SMCs was explored via 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT), 5-ethynyl-2’-deoxyuridine (EdU) staining, flow cytometry, and TdT-mediated dUTP nick end labeling (TUNEL) staining, respectively. …”
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998por Lai, Pan-Pan, Jing, Yun-Tao, Guo, Ling, Qin, Tong-Zhou, Xue, Yi-Zhe, Zhang, Zhao-Wen, Wang, Xing, Miao, Xia, Zhang, Wei, Ding, Gui-Rong“…The location of apoptotic cells was confirmed by terminal deoxynucleotidyl transferase (TdT) enzymaticated dUTP nick end labelling (TUNEL) assay. …”
Publicado 2022
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999por Zhang, Shihui, Chen, Yunyun, Hu, Qiong, Zhao, Tingting, Wang, Zhuo, Zhou, Yijian, Wei, Yuxuan, Zhao, Hongzhou, Wang, Junkai, Yang, Yaxin, Zhang, Jiaying, Shi, Songlin, Zhang, Yujun, Yang, Ling, Fu, Zhichao, Liu, Kuancan“…ABBREVIATIONS: CCK-8, Cell Counting Kit 8; Chip, Chromatin Immunoprecipitation; EC, Esophageal cancer; EMT, epithelial-to-mesenchymal transition; ESCC, Esophageal squamous cell carcinomas; LLGL2, lethal (2) giant larvae protein homolog 2; LLGL2ov, LLGL2 overexpression; MET, mesenchymal-epithelial transition; miRNAs, MicroRNAs; PRM-MS, Parallel reaction monitoring-Mass spectrometry; SD, Standard deviation; SOX, sex determining region Y (SRY)-like box; SOX2-Kd, SOX2-knockdwon; TUNEL, TdT-mediated dUTP Nick-End Labeling.…”
Publicado 2022
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1000por García-Ortiz, Humberto, Barajas-Olmos, Francisco, Flores-Huacuja, Marlen, Morales-Rivera, Monserrat I., Martínez-Hernández, Angélica, Baca, Vicente, Contreras-Cubas, Cecilia, Orozco, Lorena“…The association analysis [case-control and transmission disequilibrium test (TDT)] included 376 cases and 400 adult controls, all of whom were mestizos (MEZ). …”
Publicado 2023
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