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1021por Takeda, Reina, Yokoyama, Kazuaki, Fukuyama, Tomofusa, Kawamata, Toyotaka, Ito, Mika, Yusa, Nozomi, Kasajima, Rika, Shimizu, Eigo, Ohno, Nobuhiro, Uchimaru, Kaoru, Yamaguchi, Rui, Imoto, Seiya, Miyano, Satoru, Tojo, Arinobu“…A 65-year-old woman was initially diagnosed with Philadelphia chromosome-negative B-ALL primarily expressing TdT/CD34/HLA-DR; more than 20% of the blasts were positive for CD19/CD20/cytoplasmic CD79a/cytoplasmic CD22/CD13/CD71.The blasts were negative for T-lineage markers and myeloperoxidase (MPO). …”
Publicado 2022
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1022“…Scratch tests and transwell assays were used to detect cell migration and invasion. TdT-mediated dUTP nick-end labeling (TUNEL) staining and flow cytometry were performed to examine apoptosis and mitochondrial membrane potential. …”
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1023“…Apoptosis was detected using the terminal TdT-mediated dUTP nick end labeling (TUNEL) assay and flow cytometry (FCM). …”
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1024por Wang, Wei, Xu, Jie, Khoury, Joseph D., Pemmaraju, Naveen, Fang, Hong, Miranda, Roberto N., Yin, C. Cameron, Hussein, Siba El, Jia, Fuli, Tang, Zhenya, Hu, Shimin, Konopleva, Marina, Medeiros, L. Jeffrey, Wang, Sa A.“…We characterized their immunophenotype and genetic profiles and compared these findings with blastic plasmacytoid dendritic cell neoplasm (BPDCN). pDC-differentiation/expansion was preferentially observed in AML with an immature myeloid or myelomonocytic immunophenotype, where myeloblasts were frequently positive for CD34 (98%), CD117 (94%), HLA-DR (100%) and TdT (79%), with increased CD123 (89%) expression. …”
Publicado 2022
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1025“…The infarct volume and extent of apoptosis in brain tissues was then assessed by 2,3,5-triphenyltetrazolium (TTC) and TdT-mediated dUTP nick end labeling (TUNEL) staining. …”
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1026por Nie, Haitao, Zhang, Yuqian, Duan, Shulong, Zhang, Ying, Xu, Yunlu, Zhan, Jixiang, Wen, Yue, Wu, Xiaobing“…Furthermore, the remarkable immunohistochemical staining of proliferating cell nuclear antigen (PCNA) and B-cell lymphoma 2 (Bcl-2)-positive signals in the basilar cells, in parallel with the immuno-reactive TdT-mediated dUTP nick-End labeling(TUNEL) within suprabasal cells, provided direct molecular evidence supporting for the speculation that DG serves as a holocrine secretion mode. …”
Publicado 2022
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1027por Yang, Shu, Zhou, Xuanping, Jia, Zhen, Zhang, Mali, Yuan, Minghao, Zhou, Yizhao, Wang, Jing, Xia, Duo“…Chondrocyte apoptosis was detected by flow cytometry and TdT dUTP nick-end labeling. Signal transducer and activator of transcription 1 (STAT1), ADAMTS12, and methyltransferase-like 3 (METTL3) levels were detected by immunohistochemistry, quantitative polymerase chain reaction (qPCR), western blot, or immunofluorescence assay. …”
Publicado 2023
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1028por Zhao, Zhongwei, Yang, Qian, Zhou, Tingting, Liu, Chunhong, Sun, Manqing, Cui, Xinmu, Zhang, Xuewu“…The anti-proliferative activity of B. coagulans MZY531 was evaluated by Cell Counting Kit-8 (CCK-8) assay, and cell apoptosis was revealed with Terminal Deoxynucleotidyl Transferase (TDT)-mediated dUTP Nick-End Labeling (TUNEL) staining and flow cytometric analysis. …”
Publicado 2023
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1029por Kan, Shunli, Liu, Chengjiang, Zhao, Xinyan, Feng, Sa, Zhu, Haoqiang, Ma, Boyuan, Zhou, Mengmeng, Fu, Xuanhao, Hu, Wei, Zhu, Rusen“…Immunofluorescence with NeuN and glial fibrillary acidic protein (GFAP) was employed to evaluate neuronal survival and glial changes. TdT-mediated dUTP nick end labeling (TUNEL) assay was performed to examine apoptotic rates. …”
Publicado 2023
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1030“…We examined the impact of HIF-1α overexpression on AMI rats using Haematoxylin-Eosin (H&E) staining, TTC staining, enzyme-linked immunosorbent assay (ELISA), TdT-mediated dUTP Nick-End Labeling (TUNEL) assay, and immunohistochemistry (IHC) staining. …”
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1031por Muscarella, Lucia A, Guarnieri, Vito, Sacco, Roberto, Curatolo, Paolo, Manzi, Barbara, Alessandrelli, Riccardo, Giana, Grazia, Militerni, Roberto, Bravaccio, Carmela, Lenti, Carlo, Saccani, Monica, Schneider, Cindy, Melmed, Raun, D'Agruma, Leonardo, Persico, Antonio M“…SNPs rs72338773 and rs12939811 were not associated with autism, using either a case-control (alleles, exact P = 0.13) or a family-based design [transmission/disequilibrium test (TDT)χ(2 )= 1.774, P = 0.183]. The rare variants, all inherited from one of the parents, were present in two Italian and in two Caucasian-American families. …”
Publicado 2010
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1032por Chen, Rui, Davis, Lea K., Guter, Stephen, Wei, Qiang, Jacob, Suma, Potter, Melissa H., Cox, Nancy J., Cook, Edwin H., Sutcliffe, James S., Li, Bingshan“…Finally, we carried out rare variant group-wise transmission disequilibrium tests (gTDT) and observed significant association of rare variants in genes encoding a subset of the serotonin pathway with ASD. …”
Publicado 2017
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1033por Bhanja, Payel, Norris, Andrew, Gupta-Saraf, Pooja, Hoover, Andrew, Saha, Subhrajit“…The regenerative response of Lgr5-positive ISC was examined by lineage tracing assay using Lgr5-EGFP-ires-CreERT2-TdT mice with tamoxifen administration to activate Cre recombinase and thereby marking the ISC and their respective progeny. …”
Publicado 2018
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1034por Morishita, Miyuki, Endo, Toshiaki, Baba, Tsuyoshi, Kuno, Yoshika, Ikeda, Keiko, Kiya, Tamotsu, Honnma, Hiroyuki, Saito, Tsuyoshi“…We also examined ovarian histology to check follicle numbers by using hematoxylin-eosin staining, and the number of atretic follicles using Tdt-mediated dUTP nick end labeling (TUNEL) methods. …”
Publicado 2018
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1035por Toriuchi, Kohki, Kakita, Hiroki, Tamura, Tetsuya, Takeshita, Satoru, Yamada, Yasumasa, Aoyama, Mineyoshi“…In the presence of ACM derived from hypothermic astrocytes following OGD, the number of cleaved caspase 3 and TdT-mediated dUTP nick-end labeling-positive apoptotic neurons was lower than in the presence of ACM from normothermic astrocytes following OGD. …”
Publicado 2020
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1036Development and optimization of orthotopic liver metastasis xenograft mouse models in uveal melanomapor Sugase, Takahito, Lam, Bao Q., Danielson, Meggie, Terai, Mizue, Aplin, Andrew E., Gutkind, J. Silvio, Sato, Takami“…We also determine if hepatic tumors could be effectively monitored with non-invasive live imaging. METHODS: tdtTomate-labeled, patient-derived MUM cells were injected into the liver, spleen or tail vein of immunodeficient NSG mice. …”
Publicado 2020
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1037“…The effect of E2F1 on gastric cancer cell sensitivity of 5-Fu was evaluated using cell viability assay and TdT-mediated dUTP Nick-End Labeling staining. We also analyzed the association between E2F1 expression and clinical characteristics in gastric cancer patients. …”
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1038“…The apoptosis level of renal tissue cells was detected by TdT-mediated dUTP nick end labeling (TUNEL). 16S rDNA gene sequencing was used to detect the changes of microbial species in the contents of the duodenum and screen out the differentially expressed flora. …”
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1039“…The viability and apoptosis of transfected Huh-7 cells were, respectively, analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay. The expression of apoptosis-related proteins and HK-2 in transfected Huh-7 cells was also detected by Western blot analysis. …”
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1040por Fu, Zhonghua, Jiang, Zhengying, Guo, Guanghua, Liao, Xincheng, Liu, Mingzhuo, Xiong, Zhenfang“…The pathological changes and cell apoptosis were determined by hematoxylin-eosin (HE) staining and TdT-mediated dUTP nick end labeling (TUNEL) assay, and the PI3K/Akt/Nrf2/HO-1/NQO1, and FoxO1-NLRP3 inflammasome expression were verified by western blot (WB). …”
Publicado 2021
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