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    por Nazari, Shayan S.
    Publicado 2020
    “…Details are provided for generating 3D spheroids with a basement membrane, polymerizing collagen I, embedding the spheroids in the 3D collagen gel, and immunostaining the spheroids for invasion studies. Published 2020. U.S. Government. Basic Protocol 1: Growth of uniformly sized tumor spheroids with an encapsulating basement membrane Basic Protocol 2: Polymerization and embedding of tumor spheroids in a 3D type I collagen gel Alternate Protocol: Embedding of tumor spheroids in collagen gels using a sandwich method Basic Protocol 3: Fixing and immunostaining of tumor spheroids embedded in 3D collagen gels…”
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