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41“…U2 mutants that restore U2-BS base pairing enable dissociation of Prp5 and allows splicing to proceed. …”
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42por Guiro, Joana, Fagbemi, Mathias, Tellier, Michael, Zaborowska, Justyna, Barker, Stephanie, Fournier, Marjorie, Murphy, Shona“…ChIP-seq and ChIP-qPCR confirm the association of SPT6, Cyclin K, and CDK12 with the U2 genes. In addition, knockdown of SPT6 causes loss of subunit 3 of the Integrator Complex (INTS3) from the U2 genes, indicating a functional role in snRNA gene expression. …”
Publicado 2022
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43“…Here, we describe a protocol to stimulate the formation of ALT-associated PML bodies (APBs) and ALT activity by tethering PML-IV to telomeres in human U2OS cells. Through immunofluorescence, in situ hybridization, and microscopy, we analyze dynamics of telomere clustering, visualize recruitment of DNA repair proteins to APBs, and measure telomere DNA synthesis during ALT. …”
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47“…We investigated the transcriptomic changes in the shoot apices during floral transition in Arabidopsis mutants of two closely related splicing factors: AtU2AF65a (atu2af65a) and AtU2AF65b (atu2af65b). The atu2af65a mutants exhibited delayed flowering, while the atu2af65b mutants showed accelerated flowering. …”
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48“…The 17S U2 small nuclear ribonucleoprotein particle (snRNP) represents the active form of U2 snRNP that binds to the pre-mRNA during spliceosome assembly. …”
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49“…For splicing to proceed, U2AF has to be replaced by other factors. We show here that U2AF interacts with the signal-dependent splice regulator Sam68 and that forced expression of Sam68 results in enhanced binding of the U2AF65 subunit to an alternatively spliced pre-mRNA sequence in vivo. …”
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50por Qian, Jun, Yao, Dong-ming, Lin, Jiang, Qian, Wei, Wang, Cui-zhu, Chai, Hai-yan, Yang, Jing, Li, Yun, Deng, Zhao-qun, Ma, Ji-chun, Chen, Xing-xing“…Somatic mutations of U2AF1 gene have recently been identified in myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). …”
Publicado 2012
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51por Rehman, Sadia, Ikram, Muhammad, Baker, Robert J, Zubair, Muhammad, Azad, Effat, Min, Soyoung, Riaz, Kashif, Mok, KH, Rehman, Saeed-Ur“…The compounds were screened against U2OS cancerous cells and pathogenic micro organisms. …”
Publicado 2013
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52por Wu, Guowei, Radwan, Mohamed K., Xiao, Mu, Adachi, Hironori, Fan, Jason, Yu, Yi-Tao“…For instance, growing yeast cells to saturation induces the formation of Ψ93 in U2 snRNA. Here, we further investigate this inducible RNA modification. …”
Publicado 2016
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53por Howard, Jonathan M., Lin, Hai, Wallace, Andrew J., Kim, Garam, Draper, Jolene M., Haeussler, Maximilian, Katzman, Sol, Toloue, Masoud, Liu, Yunlong, Sanford, Jeremy R.“…One of the earliest events in this process is recognition of the 3′ splice site (3′ss) by U2 small nuclear RNA auxiliary factor 2 (U2AF2). …”
Publicado 2018
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54por Zhang, Jieying, Zhao, Huizhi, Wu, Kunlu, Peng, Yuanliang, Han, Xu, Zhang, Huan, Liang, Long, Chen, Huiyong, Hu, Jingping, Qu, Xiaoli, Zhang, Shijie, Chen, Lixiang, Liu, Jing“…U2AF1 (U2AF35) is the small subunit of the U2 auxiliary factor (U2AF) that constitutes the U2 snRNP (small nuclear ribonucleoproteins) of the spliceosome. …”
Publicado 2019
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55“…Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blot analysis were used to detect the expression of ANXA3 in osteoblasts in the osteosarcoma cell lines U2OS and HOS. Furthermore, small interfering (si)-RNA were transfected into U2OS and HOS cells via a liposome-mediated method. …”
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56“…Regardless of these solutions, there are a limited number of studies which focus on connecting layered-UAVs to everything (U2X). In particular, none of them have actually addressed the aspect of resource allocation. …”
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57“…INTRODUCTION: Although the effects of U2 small nuclear RNA auxiliary factor 1 gene (U2AF1) mutations on the outcomes of patients with myelodysplastic syndromes (MDS) have previously been investigated, their prognostic significance remains controversial. …”
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58“…For addressing this point, we parametrize NP using a gauge invariant effective field theory approach combined with a global U(2)3 flavor symmetry in the quark sector (also known as less-minimal flavor violation). …”
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59“…Conclusion: Elephant garlic exhibits antibacterial property and has an inhibitory effect on osteosarcoma cells (U2OS) proliferation and cell activity, suggesting the mechanism of its anticancer effects on U2OS human osteosarcoma cells.…”
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60por Hinteregger, Ernst, Wurst, Klaus, Perfler, Lukas, Kraus, Florian, Huppertz, Hubert“…A new actinide borate phosphate, U(2)[BO(4)][PO(4)], was synthesized in a Walker-type multianvil apparatus at 12.5 GPa and 1000 °C. …”
Publicado 2013
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