“…In particular, we focus on potential energy curves for the 1(1)Σ(u)(+), 2(1)Σ(u)(+), 1(1)Π(u), 2(1)Π(u), and 1(1)Δ(u) states computed using several variants of ab initio coupled-cluster and configuration-interaction methods to benchmark them. …”
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“…Herein, we identify a role for actopaxin phosphorylation in cell spreading and migration. Stable clones of U2OS cells expressing actopaxin wild-type (WT), nonphosphorylatable, and phosphomimetic mutants were developed to evaluate actopaxin function. …”
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“…Here, we have used FRET for an in cell study of the interaction of Ataxin-1 with the spliceosome-associated U2AF65 and the adaptor 14-3-3 proteins. Using wild-type Ataxin-1 and Ser776 mutants to a phosphomimetic aspartate and to alanine, we show that U2AF65 binds Ataxin-1 in a Ser776 phosphorylation independent manner whereas 14-3-3 interacts with phosphorylated wild-type Ataxin-1 but not with the mutants. …”
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“…Utilizing matrix-coated polydimethylsiloxane gels to manipulate substrate compliance, we show that cdGAP, an adhesion-localized Rac1 and Cdc42 specific GTPase activating protein, is necessary for U2OS osteosarcoma cells to coordinate cell shape changes and migration as a function of extracellular matrix stiffness. …”
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“…These results identify PRMT9 as a non-histone methyltransferase that primes the U2snRNP for interaction with SMN.…”
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“…The results show that a separate treatment, that is, only inhibiting viral replication (u (1)(t)) or enhancing interferon activity (u (2)(t)), has more advantages for controlling viral infection than a mixed treatment, that is, controlling both (u (1)(t)) and (u (2)(t)) simultaneously, including the smallest cost and operability. …”
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“…Complementarity to the 5′ end of exon 7 increases U2AF65 binding, but the ESE sequence is required for efficient recruitment of U2 snRNP. …”
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“…Methods: Real-time quantitive PCR was used to detect the transcription level of TUG1 in a series of osteosarcoma cell lines. Knockdown of TUG1 in U2OS cells was carried out by transient transfection of siRNAs. …”
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“…U1 subcomplex bound to the 5' splice site of an intron acts jointly with U1 bound to the 5' splice site of the next intron to dramatically increase the rate and efficiency by which U2 subcomplex is recruited to the branch site/3' splice site of the upstream intron. …”
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“…Three cells lines (WPMY-1 myofibroblasts, HT-29 colorectal adenocarcinoma, and U2OS osteosarcoma) were selected and growth in 10 ml of Minimum Essential Medium for 5 weeks with serial dilutions of sirolimus. …”
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“…The SF3b complex is an intrinsic component of the functional U2 small nuclear ribonucleoprotein (snRNP). As U2 snRNP enters nuclear pre-mRNA splicing, SF3b plays key roles in recognizing the branch point sequence (BPS) and facilitating spliceosome assembly and activation. …”
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“…Further, we have examined the cellular response of human osteoblasts (U-2 OS) on the modified substrates. The micropattern prepared with a selected combination of surface activation and amount of PMMA for honeycomb construction showed a positive effect on U-2 OS cell adhesion and proliferation. …”
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“…In human cells, the U12 spliceosome, also known as the minor spliceosome, is responsible for the splicing of 0.5% of introns, while the major U2 spliceosome is responsible for the other 99.5%. …”
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“…While the role of U2 snRNA-m(6)A(m) in splicing regulation has been reported by different independent studies, conflicting data were found for the role of cap-associated m(6)A(m) in mRNA stability and translation. …”
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“…To gain insight into the T-dependence and magnitude of H, we also estimate another more familiar measure of particle localization, the Debye–Waller parameter 〈u(2)〉 describing the mean-square particle displacement on a timescale on the order of the fast relaxation time, and we also calculate H and 〈u(2)〉 for heated crystalline Cu. …”
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“…In U2OS cell line, GABRD expression levels were significantly upregulated. …”
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“…RESULTS: The incidence rate of hematoma was significantly lower in group 2 versus group 1 (p = 0.014), the mean procedure time (minutes) and the median hospital stay (days) were significantly higher in group 1 versus group 2 (t(98) =  − 2.631, p = 0.01, and U = 2.403, p = 0.018, respectively), and the c-reactive protein pre-procedure and the c-reactive protein post-procedure were significantly lower in group 2 versus group 1 (U = -2.969, p = 0.003, and U = -2.674, p = 0.007, respectively). …”
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“…The 5 new procedures for SNP weighting were: (1) u(2) plus a constant equal to the weight of the top SNP; (2) from a heavy-tailed distribution (similar to BayesA); (3) for every 20 SNP in a window along the whole genome, the largest effect (u(2)) among them; (4) the mean effect of every 20 SNP; and (5) the summation of every 20 SNP. …”
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“…Multivariate analysis revealed that lower expression of miR-30c was associated with shorter survival of patients (P<0.01). U2OS cell growth was significantly inhibited when transfected with miR-30c mimic. …”
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“…METHODS: The expressions of miR-133b and FGFR1 were examined by RT-qPCR and compared between 30 paired normal bone tissues and OS tissues, and also between normal osteoblasts and three OS cells lines, MG-63, U2OS, and SAOS-2. Using U2OS and MG-63 as the model system, the functional significance of miR-133b and FGFR1 was assessed on cell viability, proliferation, apoptosis, migration/invasion, and epithelial–mesenchymal transition (EMT) by overexpressing miR-133b and down-regulating FGFR1 expression, respectively. …”
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