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61por Hoffmann, Julia, Symul, Laura, Shostak, Anton, Fischer, Tamás, Naef, Felix, Brunner, Michael“…U2OS cells harbor a circadian clock but express only a few rhythmic genes in constant conditions. …”
Publicado 2014
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62“…The human osteosarcoma cell line U2OS is useful for studying genome replication of human papillomavirus (HPVs) subtypes that belong to different phylogenetic genera. …”
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63por Okeyo-Owuor, Theresa, White, Brian S., Chatrikhi, Rakesh, Mohan, Dipika R., Kim, Sanghyun, Griffith, Malachi, Ding, Li, Ketkar-Kulkarni, Shamika, Hundal, Jasreet, Laird, Kholiswa M., Kielkopf, Clara L., Ley, Timothy J., Walter, Matthew J., Graubert, Timothy A.“…Although the role of U2AF1 as an accessory factor in the U2 snRNP is well established, it is not yet clear how mutations affect splicing or contribute to MDS pathophysiology. …”
Publicado 2014
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64“…BACKGROUND: The human papillomavirus (HPV) genomes can replicate, and are maintained as autonomously replicating extrachromosomal plasmids in human U2OS cells. Previous studies have shown that HPV genomes are transcriptionally active in U2OS cells and can express the viral early proteins required for initiation and establishment of HPV replication. …”
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65“…Utopia is inserted into a specific site of U2 small nuclear RNA genes with different strength of specificity for each family. …”
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66por Agrawal, Anant A., Salsi, Enea, Chatrikhi, Rakesh, Henderson, Steven, Jenkins, Jermaine L., Green, Michael R., Ermolenko, Dmitri N., Kielkopf, Clara L.“…How the essential pre-mRNA splicing factor U2AF(65) recognizes the polypyrimidine (Py) signals of the major class of 3′ splice sites in human gene transcripts remains incompletely understood. …”
Publicado 2016
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67“…The auxiliary factor of U2 small nuclear ribonucleoprotein (U2AF) facilitates branch point (BP) recognition and formation of lariat introns. …”
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68“…The branch point recognition region of spliceosomal snRNA U2 is heavily modified post-transcriptionally in most eukaryotic species. …”
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69por Zhou, Chuanman, Gao, Xiaoyang, Hu, Surong, Gan, Wenjing, Xu, Jing, Ma, Yongchao C., Ma, Long“…A key step in pre-mRNA splicing is the recognition of 3ʹ splicing sites by the U2AF large and small subunits, a process regulated by numerous trans-acting splicing factors. …”
Publicado 2018
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70RBM17 Interacts with U2SURP and CHERP to Regulate Expression and Splicing of RNA-Processing Proteinspor De Maio, Antonia, Yalamanchili, Hari Krishna, Adamski, Carolyn J., Gennarino, Vincenzo A., Liu, Zhandong, Qin, Jun, Jung, Sung Y., Richman, Ronald, Orr, Harry, Zoghbi, Huda Y.“…Here, we find that it interacts with the spliceosomal factors U2SURP and CHERP and that they reciprocally regulate each other’s stability, both in mouse and in human cells. …”
Publicado 2018
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71“…This study was focused on investigating the anticancer potential of indole-3-carbinol (I3C) against osteosarcoma MG-63 and U2OS cells. A wound healing assay indicated that IC3 inhibited migration of MG-63 and U2OS cells. …”
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72por Yoshida, Hisashi, Park, Sam-Yong, Sakashita, Gyosuke, Nariai, Yuko, Kuwasako, Kanako, Muto, Yutaka, Urano, Takeshi, Obayashi, Eiji“…The accurate exclusion of introns by RNA splicing is critical for the production of mature mRNA. U2AF1 binds specifically to the 3´ splice site, which includes an essential AG dinucleotide. …”
Publicado 2020
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73“…U2 Small Nuclear RNA Auxiliary Factor 1 (U2AF1) forms a heterodimeric complex with U2AF2 that is primarily responsible for 3ʹ splice site selection. …”
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74por Choi, Namjeong, Liu, Yongchao, Oh, Jagyeong, Ha, Jiyeon, Zheng, Xuexiu, Shen, Haihong“…A deletion mutation demonstrated that the U2 snRNP auxiliary factor 65 kDa (U2AF65) interaction domain of SF3B1 was required for its function in SMN exon 7 splicing. …”
Publicado 2020
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75por Miller, Christopher A., Walker, Jason R., Jensen, Travis L., Hooper, William F., Fulton, Robert S., Painter, Jeffrey S., Sekeres, Mikkael A., Ley, Timothy J., Spencer, David H., Goll, Johannes B., Walter, Matthew J.“…The U2AF1 gene is a core part of mRNA splicing machinery and frequently contains somatic mutations that contribute to oncogenesis in myelodysplastic syndrome, acute myeloid leukemia, and other cancers. …”
Publicado 2022
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76por Lee, Young-Sun, Kwon, Osung, Jeong, Geuk-Rae, Noh, Junyeol, Kim, Sung Eun, Yi, Gwan-Su, Hwang, Eun Mi, Park, Jae-Yong“…Here, we report that deficient TTYH1 expression results in the inhibition of the migration and invasion of U2OS human osteosarcoma cells. We found that TTYH1 was endogenously expressed at both mRNA and protein levels in U2OS cells and that these channels were located at the plasma membrane of the cells. …”
Publicado 2022
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77“…SIMPLE SUMMARY: U2 small nuclear RNA auxiliary factor 1 (U2AF1) is one of the most important RNA splicing genes involved in regulating the alternative splicing of pre-mRNA. …”
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78por Gao, Cong, Lu, Shuai, Zhou, Rong, Ding, Junjie, Fan, Jialiang, Han, Binying, Chen, Moxian, Wang, Baohua, Cao, Yunying“…BACKGROUND: Alternative splicing (AS) is an important channel for gene expression regulation and protein diversification, in addition to a major reason for the considerable differences in the number of genes and proteins in eukaryotes. In plants, U2 small nuclear ribonucleoprotein B″ (U2B″), a component of splicing complex U2 snRNP, plays an important role in AS. …”
Publicado 2022
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79U2AF1 and EZH2 mutations are associated with nonimmune hemolytic anemia in myelodysplastic syndromespor Komrokji, Rami, Aguirre, Luis E., Al Ali, Najla, Hussaini, Mohamad, Sallman, David, Rollison, Dana, Padron, Eric“…U2AF1 mutations were observed in 30% of patients with hemolysis and occurred almost exclusively at the S34 hotspot. …”
Publicado 2022
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80por Yang, Fenghua, Bian, Tong, Zhan, Xiechao, Chen, Zhe, Xing, Zhihan, Larsen, Nicolas A., Zhang, Xiaofeng, Shi, Yigong“…Three RNA helicases – DDX42, DDX46 and DHX15 – are found to be associated with human U2 snRNP, but their roles and mechanisms in U2 snRNP and spliceosome assembly are insufficiently understood. …”
Publicado 2023
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