“…Experiments were carried out with human peripheral mononuclear cells (PBMC) and the human osteosarcoma cell line U2OS. Endpoints related to DNA damage response were analysed. …”
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“…PANX3 and miR-431-5p were overexpressed in U2OS and HOS cells. The cell viability and apoptosis were determined by MTT and FITC/PI double staining assay, respectively. …”
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“…Results: For the MDA-MB-231 cells, F-actin signal performed the fluorescent antibody staining of vinculin prediction better than phase-contrast as an input. For the U2OS cells, satisfactory metrics of performance were archieved in comparison with ground truth. …”
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“…The expressions of GSK3β, p-GSK3β(Ser9), and p-GSK3β(Tyr216) in human osteoblast cells (hFOB1.19) and human osteosarcoma cells (MG63, SaOS-2, and U2-OS) were detected by Western blotting. The GSK3β activity was measured by non-radio isotopic in vitro kinase assay. …”
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“…Effects of the WEE1 inhibitor MK1775 and ATR inhibitor VE822 were investigated in U2OS osteosarcoma cells and in four lung cancer cell lines, H460, A549, H1975, and SW900, with different sensitivities to the WEE1 inhibitor. …”
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“…Here, we investigated molecular mechanisms that mediate anti-tumor effects of 15d-PGJ(2) in different OS cell lines. Human U2-OS and Saos-2 cells were treated with 15d-PGJ(2) and cell survival was measured by MTT assay. …”
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“…Western blot and immunoprecipitation were used to detect the expression of apoptotic molecules and activation of STAT3/c-Myc pathway in Saos-2 and U2SO cells. RESULTS: Diosmetin significantly inhibited cell proliferation, induced cell cycle arrest at G2/M phase and promoted cell apoptosis in both Saos-2 and U2SO cells. …”
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“…Specifically, a relatively high expression of HMGA2 was present in KHOS, MNNG/HOS, 143B and a relatively low expression was in MG63, U2OS as well as Saos‐2. HMGA2 expression is correlated with metastasis and shorter overall survival. …”
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“…We found that primary skin keratinocytes and U2OS cells expressed RIG-I, MDA5, TLR3, and STING, but not TLR7, TLR8, or TLR9. …”
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“…All protocols and software pipelines can be quickly and easily adapted for the use of alternative autophagy markers or cell types, and can also be used for high-throughput purposes.Abbreviations: AF Alexa Fluor ATG autophagy related BafA1 bafilomycin A1 BSA bovine serum albumin DAPI 4,6-diamidino-2-phenylindole DMEM Dulbecco’s modified Eagle’s medium DMSO dimethyl sulfoxide EDTA ethylenediaminetetraacetic acid EBSS Earle’s balanced salt solution FBS fetal bovine serum GFP green fluorescent protein LD lipid droplet LSM laser scanning microscope MAP1LC3B microtubule associated protein 1 light chain 3 beta MTOR mechanistic target of rapamycin kinase PBS phosphate-buffered saline PIK3C3/VPS34 phosphatidylinositol 3-kinase catalytic subunit type 3 SQSTM1 sequestosome 1 TIFF tagged image file format U2OS U-2 OS cell line WIPI WD repeat domain, phosphoinositide interacting…”
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“…The authors prove the long time stability of KAM tori (thus quasi-periodic solutions) for nonlinear Schrödinger equation \sqrt{-1}\, u_{t}=u_{xx}-M_{\xi}u+\varepsilon|u|^2u, subject to Dirichlet boundary conditions u(t,0)=u(t,\pi)=0, where M_{\xi} is a real Fourier multiplier. …”
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“…In this framework, we discuss a class of models based on the group U(2) that describe the fermion flavor structure and are compatible with an underlying GUT. …”
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“…In the crystal structure of the title compound, [U(2)Cl(4)O(4)(C(2)H(6)OS)(4)], the compound has a centrosymmetric dimeric structure bridged by two chloride anions. …”
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“…In this study, we show that morpholino oligomers complementary to the branch-site recognition elements of U2 or U12 small nuclear RNA specifically suppress the function of the two splicing systems in mammalian cells. …”
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“…According to branching formulae, which are obtained by composing representations and $\ast$-endomorphisms, it is shown that a KMS state of the CAR algebra is obtained through the above even-CAR endomorphisms from the Fock representation. A $U(2^p)$ action on ${\mathcal O}_{2^p}$ induces $\ast$-automorphisms of the CAR algebra, which are given by nonlinear transformations expressed in terms of polynomials in generators. …”
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“…According to branching formulae, which are obtained by composing representations and $\ast$-endomorphisms, it is shown that a KMS state of the CAR algebra is obtained through the above even-CAR endomorphisms from the Fock representation. A $U(2^p)$ action on ${\mathcal O}_{2^p}$ induces $\ast$-automorphisms of the CAR algebra, which are given by nonlinear transformations expressed in terms of polynomials in generators. …”
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“…According to branching formulae, which are obtained by composing representations and $\ast$-endomorphisms, it is shown that a KMS state of the CAR algebra is obtained through the above even-CAR endomorphisms from the Fock representation. A $U(2^p)$ action on ${\mathcal O}_{2^p}$ induces $\ast$-automorphisms of the CAR algebra, which are given by nonlinear transformations expressed in terms of polynomials in generators. …”
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“…According to branching formulae, which are obtained by composing representations and $\ast$-endomorphisms, it is shown that a KMS state of the CAR algebra is obtained through the above even-CAR endomorphisms from the Fock representation. A $U(2^p)$ action on ${\mathcal O}_{2^p}$ induces $\ast$-automorphisms of the CAR algebra, which are given by nonlinear transformations expressed in terms of polynomials in generators. …”
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“…According to branching formulae, which are obtained by composing representations and $\ast$-endomorphisms, it is shown that a KMS state of the CAR algebra is obtained through the above even-CAR endomorphisms from the Fock representation. A $U(2^p)$ action on ${\mathcal O}_{2^p}$ induces $\ast$-automorphisms of the CAR algebra, which are given by nonlinear transformations expressed in terms of polynomials in generators. …”
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“…The first objective of this paper is to prove the existence and uniqueness of global solutions for a Kirchhoff-type wave equation with nonlinear dissipation of the form Ku′′ + M(|A (1/2) u|(2))Au + g(u′) = 0 under suitable assumptions on K, A, M(·), and g(·). …”
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