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961por Rodrigues, Tiago B., Serrao, Eva M., Kennedy, Brett W.C., Hu, De-en, Kettunen, Mikko I., Brindle, Kevin M.“…Glycolysis in murine lymphoma and lung tumors was monitored by measuring the conversion of hyperpolarized [U-(2)H, U-(13)C]glucose to lactate using (13)C magnetic resonance spectroscopy and spectroscopic imaging. …”
Publicado 2013
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962“…A significant degree of multiple U[double bond, length as m-dash]O bonding is evident throughout the U(2)O(3) core, but either trans-,cis- or trans-,trans-OUOUO motifs can be isolated as boron- or silicon-capped oxo complexes. …”
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963por de Kernier, Isaure, Ali-Cherif, Anaïs, Rongeat, Nelly, Cioni, Olivier, Morales, Sophie, Savatier, Julien, Monneret, Serge, Blandin, Pierre“…The potential of the system to address biological questions is illustrated on white blood cell numeration in whole blood and multiwavelength imaging of the human osteosarcoma (U2-OS) cells.…”
Publicado 2019
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964“…In the present review, we will discuss how three CCCH-type zinc finger proteins, ZC3H11A, MKRN1, and U2AF1, interfere with human adenovirus replication. …”
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965“…We use the generation of endogenously AID-tagged SPT6 in U2OS cells as an example but provide sufficient details for usage in other cell types. …”
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966“…Splicing dysfunction can be independent of genetic lesions or appear as a direct consequence of mutations in components of the RNA-splicing machinery, such as in the case of mutations occurring in splicing factor genes (i.e., SF3B1, SRSF2, U2AF1) and their regulators. In addition, cancer cells exhibit marked gene expression alterations, including different usage of AS isoforms, possibly causing tissue-specific effects and perturbations of downstream pathways. …”
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967“…The steps below are specific for hTERT RPE-1 cell lines, but other adherent cell lines (e.g., U2OS, MRC-5) are also amenable for this protocol. …”
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968por Sharma, Ajit K., Fitieh, Amira Mohammed, Locke, Andrew J., Ali, Jana Yasser Hafez, Ismail, Ismail Hassan“…We describe steps to induce DSBs in U2OS osteosarcoma cells stably expressing the restriction endonucleases FokI or AsiSI. …”
Publicado 2022
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969por Dai, Quan, Pang, Li-Ting, Zhang, Fa-Lei, Wang, Gang-Qiang, Li, Xue-Mei, Liu, Ji-Kai, Feng, Tao“…Two unusual polyketide–sesquiterpene metabolites, craterodoratins T (1) and U (2), along with the known compound craterellin A (3), were isolated from the higher fungus Craterellus odoratus. …”
Publicado 2023
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970por Banerjee, Sourav, Buhrlage, Sara J., Huang, Hai-Tsang, Deng, Xianming, Zhou, Wenjun, Wang, Jinhua, Traynor, Ryan, Prescott, Alan R., Alessi, Dario R., Gray, Nathanael S.“…We find that WZ4003 and HTH-01-015 impaired the invasive potential of U2OS cells in a 3D cell invasion assay to the same extent as NUAK1 knockdown. …”
Publicado 2013
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971por Perez-Marques, Francesca, Simpson, Pippa, Yan, Ke, Quasney, Michael W., Halligan, Nadine, Merchant, Daniel, Dahmer, Mary K.“…Linkage-disequilibrium-tag single nucleotide polymorphisms (LD-tag SNPs) in genes of the following splicing factors (followed by gene name) involved in exon 9 skipping PTB1 (PTBP1), SRp40 (SFRS1), SR2/ASF (SFRS5), TDP-43 (TARDBP), TIA-1 (TIA1), and U2AF(65) (U2AF2) were genotyped. SNPs in the gene of the splicing factor CELF2 (CELF2) were selected by conservation score. …”
Publicado 2016
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972por Yu, Shibing, Fourman, Mitchell Stephen, Mahjoub, Adel, Mandell, Jonathan Brendan, Crasto, Jared Anthony, Greco, Nicholas Giuseppe, Weiss, Kurt Richard“…METHODS: Three human OS cell lines (SJSA-1, Saos-2, U-2) and two human lung cell lines (HULEC-5a, MRC-5) were cultured according to American Type Culture Collection recommendations. …”
Publicado 2017
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973“…A seven-subunit Sm protein ring assembles around specific U-rich RNA segments of the U1, U2, U4, and U5 snRNPs that direct pre-mRNA splicing. …”
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974por Ju, Cheng, Zhou, Ruihao, Sun, Jun, Zhang, Feifei, Tang, Xiaofeng, Chen, Kaddie Kwok, Zhao, Junliang, Lan, Xiaoyong, Lin, Shifan, Zhang, Zhiping, Lv, Xiao-Bin“…METHODS: We performed quantitative real time PCR to detect the expression of lncRNA SNHG5 in OS cells. 143B, MG63 (knockdown) and U2OS, U2R (overexpression) cell lines were chosen for the function study of SNHG5. …”
Publicado 2018
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975por Bereshchenko, Oxana, Lo Re, Oriana, Nikulenkov, Fedor, Flamini, Sara, Kotaskova, Jana, Mazza, Tommaso, Le Pannérer, Marguerite-Marie, Buschbeck, Marcus, Giallongo, Cesarina, Palumbo, Giuseppe, Li Volti, Giovanni, Pazienza, Valerio, Cervinek, Libor, Riccardi, Carlo, Krejci, Lumir, Pospisilova, Sarka, Stewart, A. Francis, Vinciguerra, Manlio“…MacroH2A1.1 is a target of the frequent U2AF1 S34F mutation in MDS. The role of macroH2A1.1 in hematopoiesis is unclear. …”
Publicado 2019
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976por Tristão Parra, Maíra, Esmeaeli, Naghmeh, Kohn, Jordan, Henry, Brook L., Klagholz, Stephen, Jain, Shamini, Pruitt, Christopher, Vicario, Daniel, Jonas, Wayne, Mills, Paul J.“…Patients engaging in more PA reported better physical (U = 1265.5, P = .013), functional (U = 1306.5, P = .024), and general QoL (U = 1341, P = .039), less fatigue (U = 1268, P = .014), fewer physical cancer-related symptoms (U = 2.338, P = .021), and less general distress (U = 2.061, P = .021). …”
Publicado 2020
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977por Engeltjes, Bernice, Van Dijk, Corlijn, Rosman, Ageeth, Rijke, Rudy, Scheele, Fedde, Wouters, Eveline“…It consists of five urgency levels: resuscitation and life threatening (U1), emergency (U2), urgent (U3), non-urgent (U4) and self-care advice (U5). …”
Publicado 2021
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978“…Based on mutation and X-ray evidence, we propose that −3C pairs with U2 G(31) juxtaposing the branchpoint and the 3′intron end. …”
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979por Sasa, Keita, Saito, Tsuyoshi, Kurihara, Taisei, Hasegawa, Nobuhiko, Sano, Kei, Kubota, Daisuke, Akaike, Keisuke, Okubo, Taketo, Hayashi, Takuo, Takagi, Tatsuya, Yao, Takashi, Ishijima, Muneaki, Suehara, Yoshiyuki“…The expression of XBP1s and XBP1u in OS cell lines and OS surgical samples were confirmed using qPCR. In MG63 and U2OS, toyocamycin decreased the expression level of XBP1s induced by tunicamycin. …”
Publicado 2021
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980por Giatagana, Eirini-Maria, Berdiaki, Aikaterini, Gaardløs, Margrethe, Samsonov, Sergey A., Tzanakakis, George N., Nikitovic, Dragana“…This mechanism is not valid in moderately and well-differentiated, biglycan non-expressing U-2OS and Saos-2 OS cells. ABSTRACT: Osteosarcoma (OS) is a mesenchymally derived, aggressive bone cancer. …”
Publicado 2022
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