“…Furthermore, comprehensive gene mutation and expression analyses performed using massively parallel sequencing have identified gene mutations associated with the prognosis of BCR::ABL1-negative MPNs such as ASXL1, EZH2, IDH1/2, SRSF2, and U2AF1. Furthermore, single-cell analyses have partially elucidated the effect of the order of mutation acquisition on the phenotype of BCR::ABL1-negative MPNs and the mechanism of the pathogenesis of BCR::ABL1-negative MPNs. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Crosslinked templates (xHDRTs) increase Cas9-mediated editing efficiencies by up to fivefold in K562, HEK293T, U2OS, iPS and primary T cells. Increased editing from xHDRTs is driven by events on the template molecule and requires ataxia telangiectasia and Rad3-related (ATR) kinase and components of the Fanconi anemia pathway.…”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Here, we used live-cell imaging to perform a comprehensive analysis of the effects of SiR-DNA on mitosis of four human cell lines (RPE-1, DLD-1, HeLa, and U2OS). We report a dose-, time-, and light-dependent effect of SiR-DNA on chromosome segregation. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Unexpectedly, we find that higher expression of RAR, but not RXR increases heterodimerization and chromatin binding in U2OS cells. This surprising finding indicates the limiting factor is not RXR but likely its cadre of obligate dimer binding partners. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…We employed our method on two powerful and widely used semantic segmentation networks: UNet and U2Net to train in a multi-task setup. To validate our hypothesis, we performed experiments on two different medical image segmentation data sets. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The classification report reveals the model’s proficiency in distinguishing various attack categories, including ‘Normal’, ‘DoS’ (Denial of Service), ‘Probe’, ‘U2R’ (User to Root), and ‘Sybil’. Additionally, the confusion matrix offers valuable insights into the model’s performance across these attack types. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…We find that SPF45 associates with the U2 snRNP and that mutations that remove the C-terminal end of the protein disrupt this interaction. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Furthermore, we show that inhibition of APC in U2OS cells compromises the mitotic spindle checkpoint. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Here, we report a comparison of oscillating transcription from mouse liver, NIH3T3, and U2OS cells. Several surprising observations resulted from this study, including a 100-fold difference in the number of cycling transcripts in autonomous cellular models of the oscillator versus tissues harvested from intact mice. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Using a combination of bioinformatics, cell and structural biology approaches, we have identified a UHM ligand motif (ULM), present in proteins associated with splicing, in the C-terminus of Atx1 and shown that Atx1 interacts with and influences the function of the splicing factor U2AF65 via this motif. ULM comprises S776 of Atx1 and overlaps with a nuclear localization signal and a 14-3-3 binding motif. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Core components of the spliceosome, U2 and U5 snRNPs, associated with pre-mRNA for 15–30 s, indicating that splicing is accomplished within this time period. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Overexpression of EAPP in U2OS cells increased the fraction of G1 cells and lead to heightened resistance against DNA damage- or E2F1-induced apoptosis in a p21-dependent manner. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The predictions of the theory, which can be applied using graphical methods similar to those of enzyme kinetics, are validated by obtaining internally consistent data for pair-wise analyses of three cofactors (TIF2, sSMRT, and NCoR) in U2OS cells. The analysis of TIF2 and sSMRT actions on GR-induction of an endogenous gene gave results identical to those with an exogenous reporter. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Knockdown of β2-adaptin in both U2OS osteosarcoma cells and MCF10A normal breast epithelial cells promotes the formation of matrix degrading invadopodia, adhesion structures linked to invasive migration in cancer cells. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…We compared ibandronate treatment in neoplastic human U-2 osteosarcoma and in mouse CCL-51 breast cancer cells as well as in the immortalized non-neoplastic MC3T3-E1 osteoblastic cells. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Additionally, we provide evidence of specific coilin RNase activity regulation, on both U2 and hTR transcripts, by phosphorylation of a single residue, serine 489. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…In this study, we therefore tested the dependence between the age of fullerene films (from one week to one year) and the proliferation, viability and metabolic activity of human osteosarcoma cells (lines MG-63 and U-2 OS). We also monitored potential membrane and DNA damage and morphological changes of the cells. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…RESULTS: We observed the existence of double pathogenic mutations (m.11778G>A and m.1555A>G) in two Indian LHON families, who are from different haplogroup backgrounds (M5a and U2e1), with different clinical penetrance of the disease (visual impairment). …”
Enlace del recurso
Enlace del recurso

“…We observed that the cyclin B 3'UTR was not sufficient to enhance cyclin B synthesis in human U2Os, HeLa or hTERT RPE-1 cells. A better understanding of how the equilibrium of cyclin B is regulated in mitosis may contribute to the development of improved therapeutic approaches to prevent mitotic slippage in cancer cells treated with antimitotic agents.…”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…We also found evidence that small nucleolar RNAs U2 and U12 were underrepresented in the SIV preparation, indicating that the relative number or the content of co-isolated exosomes was changed upon infection. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso