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  1. 1561
    “…The experimental design comprised predictable feeding phases (control phases: C(1), C(2) and C(3)) and unpredictable feeding phases (U(1)- signaled and U(2)- non-signaled). The animals explored more during the signaled and non-signaled unpredictable phases and during the second control phase (C(2)) than during the other two predictable phases (C(1) and C(3)). …”
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    The Effects of Feeding Unpredictability and Classical Conditioning on Pre-Release Training of White-Lipped Peccary (Mammalia, Tayassuidae)
  2. 1562
    “…To further investigate the role of DHX9 in the recognition/processing of H-DNA, we performed binding assays in vitro and chromatin immunoprecipitation assays in U2OS cells. DHX9 recognized H-DNA, as evidenced by its binding to the H-DNA structure and enrichment at the H-DNA region compared with a control region in human cells. …”
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    DHX9 helicase is involved in preventing genomic instability induced by alternatively structured DNA in human cells
  3. 1563
    “…IRIF (ionizing radiation induced foci) in U2OS cells stably expressing 53BP1-GFP were used as a surrogate marker of DSBs. …”
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    ATM Alters the Otherwise Robust Chromatin Mobility at Sites of DNA Double-Strand Breaks (DSBs) in Human Cells
  4. 1564
    “…The functionality of basic vectors for FAST assembly was tested and validated by transient expression of fluorescent model proteins in CHO, U-2-OS and HEK293 cell lines. In this test, we included many of the most common vector elements for heterologous gene expression in mammalian cells, in addition the system is fully extendable by other users. …”
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    A Versatile System for USER Cloning-Based Assembly of Expression Vectors for Mammalian Cell Engineering
  5. 1565
    “…In this study, systematic mutational analysis of the human U90 and another newly identified box C/D scaRNA, mgU2-47, demonstrated that the CB-specific accumulation of vertebrate intron-encoded box C/D scaRNAs relies on GU- or UG-dominated dinucleotide repeat sequences which are predicted to form the terminal stem-loop of the RNA apical hairpin. …”
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    Targeting vertebrate intron-encoded box C/D 2′-O-methylation guide RNAs into the Cajal body
  6. 1566
    “…This finding was confirmed in human NHDF and U2OS cells and chicken micromass culture, demonstrating the value of the SHOX-transgenic mouse for the characterization of SHOX-dependent genes and pathways in early limb development.…”
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    Identification of Novel SHOX Target Genes in the Developing Limb Using a Transgenic Mouse Model
  7. 1567
    “…METHODS: Human osteosarcoma cell lines U-2OS and Saos were exposed to increasing concentrations of paclitaxel alone or in combination with NSC23925 for 6 months. …”
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    Prevention of multidrug resistance (MDR) in osteosarcoma by NSC23925
  8. 1568
    “…GAK appears to be essential for cell death because co-administration of gefitinib and luteolin to EGFR-deficient U2OS osteosarcoma cells also had a greater effect on cell viability than administration of either compound alone. …”
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    Gefitinib and Luteolin Cause Growth Arrest of Human Prostate Cancer PC-3 Cells via Inhibition of Cyclin G-Associated Kinase and Induction of miR-630
  9. 1569
    “…Consistently, we report here that Stau1 abundance fluctuates through the cell cycle in HCT116 and U2OS cells: it is high from the S phase to the onset of mitosis and rapidly decreases as cells transit through mitosis. …”
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    Cell cycle-dependent regulation of the RNA-binding protein Staufen1
  10. 1570
    “…Drn1 interacts with Dbr1, components of the Nineteen Complex, U2 snRNA, branched intermediates, and products of splicing. …”
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    A homolog of lariat-debranching enzyme modulates turnover of branched RNA
  11. 1571
    por Koskinen, Mikko, Hotulainen, Pirta
    Publicado 2014
    “…We validate the method in U2OS cell line where the actin structures can be clearly distinguished and apply to analyze how actin filament organization in dendritic spines changes during neuronal maturation. …”
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    Measuring F-actin properties in dendritic spines
  12. 1572
    “…Notably, the engineered U2OS cells carrying an Arg296/Arg299 methylation-defective hnRNPK mutant exhibited increased apoptosis upon DNA damage. …”
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    Arginine methylation of hnRNPK negatively modulates apoptosis upon DNA damage through local regulation of phosphorylation
  13. 1573
    “…Both human osteosarcoma cell lines SOSP-9607 and U2-OS were transfected with recombinant lentiviruses to regulate miR-194 expression. …”
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    microRNA-194 suppresses osteosarcoma cell proliferation and metastasis in vitro and in vivo by targeting CDH2 and IGF1R
  14. 1574
    “…Fourth, microirradiation experiments in U2OS cells demonstrate that PARP inhibition alters the recruitment properties of XPA‐green fluorescent protein to sites of laser‐induced DNA damage. …”
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    Poly(ADP‐ribose)‐mediated interplay of XPA and PARP1 leads to reciprocal regulation of protein function
  15. 1575
    “…Snu17p exhibits an RRM domain that resembles a U2AF homology motif (UHM) and Bud13p harbors a Trp residue reminiscent of an UHM-ligand motif (ULM). …”
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    A Novel Protein-Protein Interaction in the RES (REtention and Splicing) Complex
  16. 1576
    “…Analysis of human branchpoints reveals numerous novel features, including distinct features of branchpoints for alternatively spliced exons and a family of conserved sequence motifs overlapping branchpoints we term B-boxes, which exhibit maximal nucleotide diversity while maintaining interactions with the keto-rich U2 snRNA. Different B-box motifs exhibit divergent usage in vertebrate lineages and associate with other splicing elements and distinct intron–exon architectures, suggesting integration within a broader regulatory splicing code. …”
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    Genome-wide discovery of human splicing branchpoints
  17. 1577
    “…METHODS: For detection of newborn neurons, mice were injected intraperitoneally with 5-bromo-2’-deoxyuridine (BrdU) 2h before receiving 15 tone–footshock pairings, and newborn neurons were analyzed 14 and 42 days after training. …”
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    Learning Induces Sonic Hedgehog Signaling in the Amygdala which Promotes Neurogenesis and Long-Term Memory Formation
  18. 1578
    “…Although 8-week treatment led to eventual loss of HIF1α(PP) expression, treated osteosarcoma U-2 OS cells acquired tumorigenicity in the subcutaneous tissue. …”
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    Intermittent Induction of HIF-1α Produces Lasting Effects on Malignant Progression Independent of Its Continued Expression
  19. 1579
    “…Fluorescence microscopy studies and real-time polymerase chain reaction results showed that dextran nanoparticles could deliver both miR-199a-3p and let-7a into osteosarcoma cell lines (KHOS and U-2OS) successfully. Western blotting analysis and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays demonstrated that dextran nanoparticles loaded with miRs could efficiently downregulate the expression of target proteins and effectively inhibit the growth and proliferation of osteosarcoma cells. …”
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    Polymeric nanoparticle-based delivery of microRNA-199a-3p inhibits proliferation and growth of osteosarcoma cells
  20. 1580
    “…In addition, intronic pAs are substantially regulated by splicing factors, with U1 mostly inhibiting C/P events in introns near the 5’ end of gene and U2 suppressing those in introns with features for efficient splicing. …”
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    Systematic Profiling of Poly(A)+ Transcripts Modulated by Core 3’ End Processing and Splicing Factors Reveals Regulatory Rules of Alternative Cleavage and Polyadenylation
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