“…The knockdown of Arp4 (Arp4 KD) led to an increase in nuclear F-actin formation in NIH3T3 cells, and purified Arp4 potently inhibited F-actin formation in mouse nuclei transplanted into Xenopus laevis oocytes. Consistently, Arp4 KD facilitated F-actin-inducible gene expression (e.g., OCT4) and DNA damage repair. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…In addition, we found that cockroach Pameα7 subunits expressed in Xenopus laevis oocytes can assemble to form homomeric receptors. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Further, vwde expression is tightly linked to the ability to regenerate appendages in Xenopus laevis. Functional experiments demonstrate a requirement for vwde in regeneration and indicate that Vwde is a potent growth factor in the blastema. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Here, we demonstrate that eggs from amphibia and teleost fish also release zinc. Using Xenopus laevis as a model, we document that zinc reversibly blocks fertilization. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…We performed a functional characterization of Am_Eglp 1 using heterologous expression in Xenopus laevis oocyte followed by water uptake assays. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Injection of this rad21 variant mRNA into Xenopus laevis embryos disrupted the organization of corneal stroma fibrils. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The transcriptome analysis of injured Xenopus laevis tadpole and mice suggested that Neurod4L.S., a basic-helix-loop-helix transcription factor, was the most promising transcription factor to exert neuroregeneration after spinal cord injury (SCI) in mammals. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Here, we demonstrate biochemically using Xenopus laevis egg extracts that the Cdk1-counter-acting phosphatase PP2A-B55 functions as a bistable switch, even when the bistability of Cdk1 activation is suppressed. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Here, we use nucleoplasmic extract derived from the eggs of Xenopus laevis to investigate the role of BRCA1 in a cell-free transcription system. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…In this study, we analyzed possible effects of propacetamol, or its metabolite N,N-diethylglycine (DEG), on GlyRs or GlyTs function by using a two-electrode voltage clamp approach in Xenopus laevis oocytes. Our data demonstrate that, although propacetamol or acetaminophen had no effect on the function of the analysed glycine-responsive proteins, the propacetamol metabolite DEG acted as a low-affine substrate for both GlyT1 (EC50 > 7.6 mM) and GlyT2 (EC50 > 5.2 mM). …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Using Xenopus Metamorphosis Assay (XEMA) protocol, this study assessed the thyroidal effects of environmentally relevant concentrations of Diquat dibromide at 0.05, 0.11, and 0.14 mg/L on Xenopus laevis metamorphosis. The formulation significantly reduced both the fore and hind limb lengths, and disrupted the developmental stage at concentrations of 0.11 and 0.14 mg/L, with a median at NF-stage 57, while median of NF-stage 60 was recorded in the control. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…We used GC-MS and LC-MS for metabolic screening of Xenopus laevis oocytes expressing various human amino acid transporters incubated in complex media to establish their comprehensive substrate profiles. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Three versions of DHCR7 originating from different organisms—including the DHCR7 from Physalis angulata (PhDHCR7) newly discovered in this study, as well as the previously reported OsDHCR7 from Oryza sativa and XlDHCR7 from Xenopus laevis—were assessed for their ability to produce 24-methylene-cholesterol. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Here, we use cryogenic electron microscopy to determine structures of recombinant Xenopus laevis CRL2(Lrr1) with and without neddylation. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…However, the mechanisms by which RNAs and proteins interact to promote RNP granule structure and function in vivo remain unclear. In Xenopus laevis oocytes, maternal mRNAs are localized as large RNPs to the vegetal hemisphere of the developing oocyte, where local translation is critical for proper embryonic patterning. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…When human AQPs were expressed in Xenopus laevis oocytes, the results of the swelling assay showed that boric acid permeability significantly increased in oocytes expressing AQP3, 7, 8, 9, and 10, but not in those expressing AQP1, 2, 4, and 5. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…We have analyzed mutagenesis in Xenopus laevis and Drosophila melanogaster early embryos. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Here, to elucidate the mechanism underlying such a broad substrate specificity as that of AP endonucleases, we performed a computational study of four homologous APE1-like endonucleases: insect (Drosophila melanogaster) Rrp1, amphibian (Xenopus laevis) APE1 (xAPE1), fish (Danio rerio) APE1 (zAPE1), and human APE1 (hAPE1). …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The following customizations to traditional tissue explant generation procedures have facilitated the successful generation of adherent skin epithelial-like cell lines from Xenopus laevis and can be further adapted for use with different frog species, such as Rana sylvatica, and different tissues: • Osmotic adjustment of culture medium and solutions for different amphibian species. • Use of small tissue explants, instead of enzymatic digestion of tissues, and gentle spotting of these tissue explants onto the growth surface of tissue culture flasks to promote better tissue adherence. • Partial replacement of medium to allow accumulation of potential endogenous growth factors in cultures.…”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Two different E3 ubiquitin ligases have now been shown to ubiquitylate the helicase under different conditions: Cul2(Lrr1) and TRAIP. Here, using Xenopus laevis egg extract cell-free system and biochemical approaches, we have found two p97 cofactors, Ubxn7 and Faf1, which can interact with p97 during replisome disassembly during S-phase. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso