“…The factors involved have been partially identified and are poorly characterised. Here, using Xenopus laevis egg extracts we show that RNAs are required for proper nuclear envelope assembly following sperm DNA decondensation. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Importantly, mutant BuGZ that disrupts the coacervation activity in vitro fails to promote AurA phosphorylation in Xenopus laevis egg extracts. These results suggest that BuGZ coacervation promotes AurA activation in mitosis.…”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Existing heterologous expression systems that have been used to functionally characterize plant CCC proteins, namely yeast and Xenopus laevis oocytes, have limitations that are discussed. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Studies in multiple systems, including Xenopus laevis epidermis, murine trachea, and zebrafish kidney, have uncovered a transcriptional network that regulates multiple steps of multiciliogenesis, ultimately leading to an MCC with hundreds of motile cilia extended from their apical surface, which beat in a coordinated fashion. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Microinjection of additional XMAP215 protein into Xenopus laevis embryos was sufficient to induce the assembly of larger spindles during developmental stages 6.5, 7, and 8, whereas addition of a polymerase-incompetent XMAP215 mutant resulted in a downward shift in the in vivo spindle scaling curve. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…To determine augmin’s architecture and function, we purified Xenopus laevis augmin from insect cells. We demonstrate that augmin is sufficient to target γ-TuRC to MTs by in vitro reconstitution. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Except for the aspirin and acyclovir prodrugs, which were unstable in the assay conditions and were not further studied, the prodrugs were tested for affinity and transport by PepT1 expressed in Xenopus laevis oocytes: binding affinities ranged from approximately 0.1 to 2 mM. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Here, we characterized barley CNGC2-3 (HvCNGC2-3) by the two-electrode voltage-clamp technique in the Xenopus laevis oocyte heterologous expression system. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Here, to address this problem, we focus our efforts on finding conditions that allow reliable AP recordings from Xenopus laevis oocytes coexpressing Na(+) and K(+) channels. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Here, we characterized the localization of all 15 formins in epithelial cells of Xenopus laevis gastrula-stage embryos. Dia1 and Dia2 localized to tight junctions, while Fhod1 and Fhod3 localized to adherens junctions. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…In this study, we investigated emerin’s impact on nuclei assembled in Xenopus laevis egg extract, a simplified biochemical system that lacks potentially confounding cellular factors and activities. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…We have performed electrophysiological recordings on Xenopus laevis oocytes, finding rapid (time constants, 50 μs to 5 ms) photoinduced transient changes in the range of 20 to 110 mV. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Here we studied the expression dynamics of all hox genes of the diploid species Xenopus tropicalis in four Hox clusters (A–D) by analyzing high-temporal-resolution RNA-seq databases and the results showed that temporal collinearity is not supported, which is consistent with our previous data from allotetraploid Xenopus laevis. Because the temporal collinearity hypothesis implicitly assumes the collinear order of gene activation, not mRNA accumulation, we determined for the first time the timing of when new transcripts of hox genes are produced, by detecting pre-spliced RNA in whole embryos with reverse transcription and quantitative PCR (RT-qPCR) for all hoxa genes as well as several selected hoxb, hoxc and hoxd genes. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Here, we show that DAPLE, a non-GPCR protein linked to human neurodevelopmental disorders, is expressed specifically in the neural plate of Xenopus laevis embryos to trigger a G protein signaling pathway that promotes apical cell constriction during neurulation. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Here, we test for an effect of acclimation on locomotor performance traits (jump force and stamina) in adults of two predominantly aquatic and closely related frog species from different climatic regions, Xenopus tropicalis (tropical) and Xenopus laevis (temperate). We find significant effects of acclimation temperature on exertion capacity and for jump force in X. tropicalis but no effect of acclimation temperature on burst performance in X. laevis. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Although extensively studied, there are still significant gaps regarding the proteins involved in regulating ciliogenesis. Using the Xenopus laevis embryo, we show that Dishevelled (Dvl), a key Wnt signaling scaffold that is critical to proper ciliogenesis, interacts with Drg1 (developmentally regulated GTP-binding protein 1). …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…In this paper we combine experiments performed in untreated and in progesterone-treated Xenopus laevis oocytes and mathematical models to investigate how the interplay between geometry (the IP(3)R spatial distribution) and dynamics (the processes that characterize the release, transport, and removal of cytosolic Ca(2+)) affects the resulting signals. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Here we have developed improved methods for simultaneously preserving F-actin and microtubules in fixed samples and exploited them to demonstrate that F-actin is indeed associated with mitotic spindles in intact Xenopus laevis embryonic epithelia. We also find that there is an “F-actin cycle,” in which the distribution and organization of spindle F-actin changes over the course of the cell cycle. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Reductions in both cell and nuclear sizes during Xenopus laevis embryogenesis provide a robust scaling system to study mechanisms of nuclear size regulation. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Xenopus laevis frogs are a widely used organism to study aspects of modern biology (Harland and Grainger, 2011). …”
Enlace del recurso
Enlace del recurso
Enlace del recurso