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1341por Richter, K., Mathes, V., Fronius, M., Althaus, M., Hecker, A., Krasteva-Christ, G., Padberg, W., Hone, A. J., McIntosh, J. M., Zakrzewicz, A., Grau, V.“…In stark contrast to choline, phosphocholine does not evoke ion current responses in Xenopus laevis oocytes, which heterologously express functional homomeric nAChR composed of α9 subunits or heteromeric receptors containing α9 and α10 subunits. …”
Publicado 2016
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1342“…Heterologous expression of AtSLAH1 in Xenopus laevis oocytes led to no detectible transport, suggesting the need for post-translational modifications for AtSLAH1 to be active. …”
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1343por Truszkowski, Torrey L. S., James, Eric J., Hasan, Mashfiq, Wishard, Tyler J., Liu, Zhenyu, Pratt, Kara G., Cline, Hollis T., Aizenman, Carlos D.“…METHODS: We knocked down FMRP expression using morpholino oligos in the optic tectum of Xenopus laevis tadpoles and performed a series of behavioral and electrophysiological assays. …”
Publicado 2016
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1344por Ying, Guoxin, Gerstner, Cecilia D., Frederick, Jeanne M., Boye, Sanford L., Hauswirth, William W., Baehr, Wolfgang“…Rab11a and Rab8a are ubiquitous small GTPases shown as required for rhodopsin transport in Xenopus laevis and zebrafish photoreceptors by dominant negative (dn) disruption of function. …”
Publicado 2016
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1345por Rutherford, Erin L., Carandang, Leslie, Ebbert, Patrick T., Mills, Alexandra N., Bowers, Jackson T., Lowery, Laura Anne“…We previously showed that TACC1 and TACC3, members of the transforming acidic coiled-coil protein family, can act as +TIPs to regulate MT dynamics in Xenopus laevis. Here we characterize TACC2 as a +TIP that localizes to MT plus ends in front of EB1 and overlapping with TACC1 and TACC3 in multiple embryonic cell types. …”
Publicado 2016
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1346por Inoue, Yasuhiro, Suzuki, Makoto, Watanabe, Tadashi, Yasue, Naoko, Tateo, Itsuki, Adachi, Taiji, Ueno, Naoto“…To validate the model, apical constriction and cell elongation were inhibited in Xenopus laevis embryos. The resulting cell and tissue shapes resembled the corresponding simulation results. …”
Publicado 2016
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1347por Cortes, Pablo A., Puschel, Hans, Acuña, Paz, Bartheld, José L., Bozinovic, Francisco“…In this study, we tested the effect of thermal acclimation on thermal tolerance and locomotor performance in the invasive Xenopus laevis and the Chilean native Calyptocephalella gayi. …”
Publicado 2016
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1348por Marken, John P., Halleran, Andrew D., Rahman, Atiqur, Odorizzi, Laura, LeFew, Michael C., Golino, Caroline A., Kemper, Peter, Saha, Margaret S.“…We applied our and other commonly used calcium analysis methods on a dataset from Xenopus laevis neural progenitors which displays irregular calcium activity and a dataset from murine synaptic neurons which displays activity time series that are well-described by visually-distinguishable features. …”
Publicado 2016
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1349Quantifying the relative contributions of different solute carriers to aggregate substrate transportpor Taslimifar, Mehdi, Oparija, Lalita, Verrey, Francois, Kurtcuoglu, Vartan, Olgac, Ufuk, Makrides, Victoria“…Data input were the normalized measured uptake of the essential neutral amino acid (AA) L-leucine (Leu) from concentration-dependence assays performed using Xenopus laevis oocytes. Our methodology was verified by calculating Leu and L-phenylalanine (Phe) data in the presence of competitive substrates and/or inhibitors. …”
Publicado 2017
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1350por Erdogan, Burcu, Cammarata, Garrett M., Lee, Eric J., Pratt, Benjamin C., Francl, Andrew F., Rutherford, Erin L., Lowery, Laura Anne“…RESULTS: Here, we show that TACC3 is required to promote persistent axon outgrowth and prevent spontaneous axon retractions in embryonic Xenopus laevis neurons. We also show that overexpressing TACC3 can counteract the depolymerizing effect of low doses of nocodazole, and that TACC3 interacts with MT polymerase XMAP215 to promote axon outgrowth. …”
Publicado 2017
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1351“…The widespread African clawed frog (Xenopus laevis) occurs in sympatry with the IUCN Endangered Cape platanna (Xenopus gilli) throughout its entire range in the south-western Cape, South Africa. …”
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1352“…We investigated the ontogeny of leptin mRNA, leptin actions on cell proliferation in the brain, and gene expression in the preoptic area/hypothalamus of tadpoles of Xenopus laevis. The level of leptin mRNA was low in premetamorphic tadpoles, but increased strongly at the beginning of metamorphosis and peaked at metamorphic climax. …”
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1353por Kepchia, Devin, Moliver, Scott, Chohan, Kunal, Phillips, Cameron, Luetje, Charles W.“…Orco antagonist activity was assessed on ORs from Drosophila melanogaster flies and Culex quinquefasciatus mosquitoes, expressed in Xenopus laevis oocytes and assayed by two-electrode voltage clamp electrophysiology. …”
Publicado 2017
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1354“…Functional assays in mouse show cannabinoid sensitivity changes and Cnrip1 has recently been suggested to control eye development in Xenopus laevis. Two Cnrip1 genes are described in zebrafish, cnrip1a and cnrip1b. …”
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1355por Chen, Gong, Wang, Wei, Chen, Hongli, Dai, Weijiang, Peng, Xiangran, Li, Xiaoliang, Tang, Xudong, Xu, Li, Shen, Zhongyuan“…After expression of NbAQP in Xenopus laevis oocytes, it was observed that NbAQP can promote rapid penetration of water into oocytes. …”
Publicado 2017
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1356“…ORs were expressed in Xenopus laevis oocytes and assayed by two-electrode voltage clamp electrophysiology. …”
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1357por Sakamaki, Kazuhiro, Iwabe, Naoyuki, Iwata, Hiroaki, Imai, Kenichiro, Takagi, Chiyo, Chiba, Kumiko, Shukunami, Chisa, Tomii, Kentaro, Ueno, Naoto“…Overexpression of a truncated mutant of c-FLIP in the Xenopus laevis embryos by mRNA microinjection caused thorax edema and abnormal constriction of the abdomen. …”
Publicado 2015
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1358por Garrood, William T., Zimmer, Christoph T., Gutbrod, Oliver, Lüke, Bettina, Williamson, Martin S., Bass, Chris, Nauen, Ralf, Emyr Davies, T.G.“…To analyse the roles of these mutations in resistance to phenylpyrazoles, three Rdl constructs: wild type, A301S and A301S + Q359E were expressed in Xenopus laevis oocytes and assessed for their sensitivity to ethiprole and fipronil using two-electrode voltage-clamp electrophysiology. …”
Publicado 2017
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1359por Gorraitz, Edurne, Hirayama, Bruce A., Paz, Aviv, Wright, Ernest M., Loo, Donald D. F.“…The mutants were expressed in Xenopus laevis oocytes and tagged with environmentally sensitive fluorescent rhodamines [e.g., tetramethylrhodamine (TMR)-thiols]. …”
Publicado 2017
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1360“…Proper migration of these cells requires proteolysis of cell adhesion molecules, such as cadherins. In Xenopus laevis, preventing extracellular cleavage of cadherin-11 impairs CNC migration. …”
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