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  1. 30261
    “…Here, we apply a hybrid sequencing approach relying on the combination of short- and long-read next-generation sequencing platforms and whole-genome restriction analysis by using pulsed-field gel electrophoresis (PFGE) to produce a high-quality reference genome for this biotechnologically important bacterium. By using PFGE to separate and isolate plasmid DNA from chromosomal DNA, we successfully sequenced the GLP using Nanopore data alone. …”
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    Reference-Grade Genome and Large Linear Plasmid of Streptomyces rimosus: Pushing the Limits of Nanopore Sequencing
  2. 30262
    “…RapidFor™ SARS-CoV-2 antigen test (Vitrosens Biotechnology, Istanbul, Turkey) was used to investigate the presence of SARS-CoV-2 antigen in all samples following the manufacturer’s instructions. …”
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    Comparison of a novel antigen detection test with reverse transcription polymerase chain reaction assay for laboratory diagnosis of SARS-CoV-2 infection
  3. 30263
    “…Expression of HDAC1 (binding target of S1P) and genes associated with the sphingomyelin (SM) pathway were examined in datasets identified in the National Centre for Biotechnology Information, Gene Expression Omnibus, and analysed using the R2: Genomics Analysis and Visualization Platform (http://r2.amc.nl). …”
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    DIPG-50. Bioinformatic evaluation of genes involved in sphingomyelin biosynthesis in Diffuse Midline Glioma H3K27 altered/DIPG: dysregulation of sphingosine 1-phosphate (SP1)
  4. 30264
    “…Subsequent aggregated analysis of variables measured across multiple volunteers will enable us to further understand factors which may underpin differential response to dietary interventions. FUNDING SOURCES: Biotechnology and Biological Sciences Research Council (BBSRC) UK and Unilever Foods Innovation Centre, Wageningen, The Netherlands: Collaborative Training Partnership (CTP) PhD.…”
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    Examining Individual Factors Influencing Adherence and Response to Wholegrains and Nuts in a Series of Interventional N-of-1 Studies
  5. 30265
    “…BACKGROUND: Contamination−free culture is a prerequisite for the success of in vitro − based plant biotechnology. Aseptic initiation is an extremely strenuous stride, particularly in woody species. …”
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    Establishment of optimized in vitro disinfection protocol of Pistacia vera L. explants mediated a computational approach: multilayer perceptron–multi−objective genetic algorithm
  6. 30266
    “…Knowledge of insect CRMs is also an important step in developing biotechnology methods for control of insect disease vectors and for eliminating pathogen transmission. …”
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    REDfly: An Integrated Knowledgebase for Insect Regulatory Genomics
  7. 30267
    “…It currently includes gene information for over 38,000 different taxa retrieved from the National Center for Biotechnology and Information (NCBI) Gene resource. Supported databases of gene IDs include NCBI Gene Symbols, NCBI Gene IDs (Entrez IDs), OMIM IDs, HGNC IDs, Ensembl IDs, and 28 other taxa-specific identifiers. …”
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    GeneToList: A Web Application to Assist with Gene Identifiers for the Non-Bioinformatics-Savvy Scientist
  8. 30268
    “…An approach to improving mesh-tissue integration is coating PPMs with anti-inflammatory and wound-healing molecules, such as platelet-rich plasma (PRP), which is a component of biotechnologies that are capable of accelerating wound healing. …”
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    Mesh-Tissue Integration of Platelet-Rich Plasma–Decellularized Amnion Scaffold–Polypropylene Mesh Sandwiches Implanted in the Vesicovaginal Spaces of Hypoestrogenic Rabbit Models: Protocol for a Randomized Controlled Trial
  9. 30269
    “…The GeneCards, National Center for Biotechnology Information, and Online Mendelian Inheritance in Man databases were used to compile information on the diseases hepatitis, adenovirus, and SARS-CoV-2. …”
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    Potential protective benefits of Schisandrin B against severe acute hepatitis in children during the COVID-19 pandemic based on a network pharmacology analysis
  10. 30270
    “…SIMPLE SUMMARY: The characterization of microbial communities from Antarctic temporary meltwater ponds is limited, while they could serve as a source of biotechnologically interesting microorganisms. In this study, we characterized a set of bacteria isolated from the sea-affected temporary meltwater ponds in the East Antarctica area of the Vecherny region of the Thala Hills Oasis, Enderby Land. …”
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    Isolation, Physiological Characterization, and Antibiotic Susceptibility Testing of Fast-Growing Bacteria from the Sea-Affected Temporary Meltwater Ponds in the Thala Hills Oasis (Enderby Land, East Antarctica)
  11. 30271
    “…Researchers have used various forms of biotechnology and human, animal, and cellular models to better understand the relationship of ALPK1 with cancer and cancer-related inflammatory diseases. …”
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    Systematic Review of the Role of Alpha-Protein Kinase 1 in Cancer and Cancer-Related Inflammatory Diseases
  12. 30272
    “…Bacillus subtilis is a biotechnologically important bacterium that serves as a model for studying biofilms. …”
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    Kin Discrimination Modifies Strain Distribution, Spatial Segregation, and Incorporation of Extracellular Matrix Polysaccharide Mutants of Bacillus subtilis Strains into Mixed Floating Biofilms
  13. 30273
    “…METHODS: Microarrays data of human CRC with liver metastasis (CRCLM) were downloaded from the National Center for Biotechnology Information (NCBI) Gene Expression Omnibus (GEO) database to identify potential key genes. …”
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    Construction of a co-expression network and prediction of metastasis markers in colorectal cancer patients with liver metastasis
  14. 30274
    “…Bb5, Bb6, Bb8, Bb12, Bb15, and Bb21 strains were identified as B. bassiana by sequencing of rDNA-ITS and TEF segments and sequence comparison to NCBI (National Center for Biotechnology Information) GenBank. Out of the six strains tested for pathogenicity, Bb6, Bb12, and Bb15 strains outperformed against T. truncatus with LC(50) values 1.4×10(6), 1.7×10(6), and 1.4×10(6) and with a LC(90) values 7.3×10(7), 1.4×10(8), and 4.2×10(8) conidia/ml, respectively, at 3 days after inoculation and were considered as potential strains for effective mite control. …”
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    “Metabolomic diversity of local strains of Beauveria bassiana (Balsamo) Vuillemin and their efficacy against the cassava mite, Tetranychus truncatus Ehara (Acari: Tetranychidae)”
  15. 30275
    “…In this study, we described the nature of discordance in a sample of 80 variants from a list of variants requiring updating by InSiGHT for ClinGen by comparing their existing InSiGHT classifications with the various submissions for each variant on the US National Centre for Biotechnology Information's (NCBI) ClinVar database. …”
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    Evaluation of literature searching tools for curation of mismatch repair gene variants in hereditary colon cancer
  16. 30276
    “…Gene functions were annotated using the National Center for Biotechnology Information (NCBI) non-redundant protein, Swiss-Prot, Kyoto Encyclopedia of Genes and Genomes, Clusters of Orthologous Groups of proteins, Gene Ontology, Pathogen–Host Interactions, Carbohydrate-Active enZYmes, and Rapid Annotations using Subsystem Technology databases, revealing non-ribosomal pathways associated with antifungal mechanisms, biofilm formation, chemotactic motility, VOC 3-hydroxy-2-butanone, cell wall-associated enzymes, and synthesis of various secondary metabolites. antiSMASH analysis predicted that CTXW 7-6-2 can produce the active substances bacillaene, bacillibactin, subtilosin A, bacilysin, and luminmide and has four gene clusters of unknown function. …”
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    Biocontrol potential of Bacillus subtilis CTXW 7-6-2 against kiwifruit soft rot pathogens revealed by whole-genome sequencing and biochemical characterisation
  17. 30277
    “…This study provides effective tools that can be employed to smoothly adjust the erythritol-inducible co-expression of two target genes in biotechnology applications. BDPs developed in this study have potential applications in the fields of heterologous protein production, metabolic engineering, and synthetic biology. …”
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    Bidirectional hybrid erythritol-inducible promoter for synthetic biology in Yarrowia lipolytica
  18. 30278
    por Zhong, Junqing, Xiang, Ding, Ma, Xinlong
    Publicado 2023
    “…METHODS: The GSE169077 gene chip dataset was downloaded from the public gene chip data platform of the National Biotechnology Information Center. The dataset included 6 samples of OA tissues and 5 samples of healthy cartilage tissues. …”
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    Prediction and analysis of osteoarthritis hub genes with bioinformatics
  19. 30279
    “…Fabrication of micro- and nanofibers are critical for a wide range of applications from microelectronics to biotechnology. Alginate microfibers with diameters of tens to hundreds of microns play an important role in tissue engineering and fibers of these diameters are impossible to fabricate via electrospinning and can only be produced via fluidic spinning. …”
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    Dissolvable Calcium Alginate Microfibers Produced via Immersed Microfluidic Spinning
  20. 30280
    “…Government funded grants were scored as directly, indirectly, or not likely related to four key innovations underlying mRNA covid-19 vaccines—lipid nanoparticle, mRNA synthesis or modification, prefusion spike protein structure, and mRNA vaccine biotechnology—on the basis of principal investigator, project title, and abstract. …”
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    US public investment in development of mRNA covid-19 vaccines: retrospective cohort study
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