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  1. 31061
    “…Our results can guide the improvement of conifer somatic embryogenesis technology and can provide a theoretical basis for accelerating the application of biotechnology in large-scale artificial breeding.…”
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    Morphological and Physiological Indicators for Screening Cell Lines with High Potential for Somatic Embryo Maturation at an Early Stage of Somatic Embryogenesis in Pinus Koraiensis
  2. 31062
    “…The diagnosis of HCTDs is aided by genomic biotechnologies (e.g., next-generation sequencing panels) facilitating the discovery of novel variants causing disease. …”
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    Clinical genetics evaluation and testing of connective tissue disorders: a cross-sectional study
  3. 31063
    “…METHODS: We employed the Search Tag Analyze Resource for Gene Expression Omnibus platform to search the The National Center for Biotechnology Information Gene Expression Omnibus to elucidate NAFLD and NASH pathology. …”
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    Transcriptome changes in stages of non-alcoholic fatty liver disease
  4. 31064
    “…OBJECTIVE: The study aimed at isolation of CD117(+) stem cells from amniotic fluid samples followed by their invitro differentiation towards nephron progenitors that can be potentially used for regenerative medicine studies and to further understand pathways involved in renal pathogenesis METHODS: This experimental study was conducted at Dow Research Institute of Biotechnology and Biomedical Sciences (DRIBBS), Dow University of Health Sciences, OJHA Campus Karachi from November 2019 to December 2020. …”
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    Differentiation of CD117(+) Amniotic Fluid Stem Cells towards Nephron Progenitors
  5. 31065
    “…RNA-seq data of 11 wheat tissues were obtained from the National Center for Biotechnology Information (NCBI), including the stamens of CSTP and the pistils and pistillodic stamen of HTS-1. …”
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    Co-expression network analysis of genes and networks associated with wheat pistillody
  6. 31066
    “…BACKGROUND: Climate change caused by greenhouse gas emission has become a global hot topic. Although biotechnology is considered as an environmentally friendly method to produce chemicals, almost all biochemicals face carbon dioxide emission from inevitable respiration and energy metabolism of most microorganisms. …”
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    In situ carbon dioxide capture to co-produce 1,3-propanediol, biohydrogen and micro-nano calcium carbonate from crude glycerol by Clostridium butyricum
  7. 31067
    “…The databases/web search engines that will be used are IEEE Xplore, PubMed, Scopus, Google Scholar, National Center for Biotechnology Information, SAGE Journals, and ScienceDirect. …”
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    Technologies for Interoperable Internet of Medical Things Platforms to Manage Medical Emergencies in Home and Prehospital Care: Protocol for a Scoping Review
  8. 31068
    “…The molecular identity of the strains was confirmed by matching 16S rRNA sequences of bacterial strains with the gene sequences of type strains available in the National Center for Biotechnology Information database and reported 38 different species of Bacillus in all the five wheat cultivars. …”
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    Diversity and Exploration of Endophytic Bacilli for the Management of Head Scab (Fusarium graminearum) of Wheat
  9. 31069
    “…Thus, we constructed a circRNA-miRNA-mRNA network based on the ceRNA hypothesis by analyzing data from the National Center for Biotechnology Information Gene Expression Omnibus (NCBI-GEO) database. …”
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    Reconstruction and Differential Expression Profiling Core Target Analyses of the circRNA-miRNA-mRNA Network Based on Competitive Endogenous RNAs in Ulcerative Colitis
  10. 31070
    “…This protocol which used simple mixture of commercial enzymes is highly reproducible and can be applied in biotechnology research on cassava.…”
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    Plant regeneration from leaf mesophyll derived protoplasts of cassava (Manihot esculenta Crantz)
  11. 31071
    “…Today, itaconic acid is biotechnologically produced with Aspergillus terreus at industrial scale from sugars. …”
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    Metabolic engineering of Corynebacterium glutamicum for acetate-based itaconic acid production
  12. 31072
    “…Thus, competent cells are suitable for different cloning jobs and should help with the increased demand for DNA assembly in biological studies and biotechnology. IMPORTANCE DNA transformation is commonly used in cloning; however, high transformation efficiency becomes a limiting factor in many applications, such as the construction of CRISPR and DNA libraries, the assembly of multiple fragments, and the transformation of large plasmids. …”
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    An Optimized Transformation Protocol for Escherichia coli BW3KD with Supreme DNA Assembly Efficiency
  13. 31073
    “…Because of the lack of modern biotechnology research, C. oleifera faces huge challenges in both breeding and basic research. …”
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    Isolation, purification and PEG-mediated transient expression of mesophyll protoplasts in Camellia oleifera
  14. 31074
    “…Thus, its efficient degradative system provides a great platform for developing sustainable biotechnologies for biomass conversions. However, progress has been hampered by the lack genetic tools facilitating mechanistic studies and engineering applications. …”
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    A Laccase Gene Reporting System That Enables Genetic Manipulations in a Brown Rot Wood Decomposer Fungus Gloeophyllum trabeum
  15. 31075
    “…While recombinant good manufacturing practices production technologies are required to produce clinical grade mAbs, academic laboratories and biotechnology companies still rely on the original hybridoma lines to stably and effortlessly produce high antibody yields at a modest price. …”
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    CRISPR/Cas9-based Engineering of Immunoglobulin Loci in Hybridoma Cells
  16. 31076
    “…These local ram breeds have low seasonality and can be employed in natural mating as well as semen donors for cryopreservation and assisted reproductive biotechnologies during the whole year at our latitude.…”
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    Ram Semen Cryopreservation for Portuguese Native Breeds: Season and Breed Effects on Semen Quality Variation
  17. 31077
    “…METHODS: First, the proteomes of 6 Nocardia subspecies Nocardia subspecies (Nocardia farcinica, Nocardia cyriacigeorgica, Nocardia abscessus, Nocardia otitidiscaviarum, Nocardia brasiliensis and Nocardia nova) were download NCBI (National Center for Biotechnology Information) database on May 1st, 2022 for the target proteins selection. …”
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    Design of a multi-epitope vaccine against six Nocardia species based on reverse vaccinology combined with immunoinformatics
  18. 31078
    “…His group develops new materials for nano- and biotechnology. The materials include glasses, ceramics, and nanoporous substrates for biological membranes.…”
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    Biomembrane Frontiers: Nanostructures, Models, and the Design of Life
  19. 31079
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    Network Proximity-based computational pipeline identifies drug candidates for different pathological stages of Alzheimer's disease
  20. 31080
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    Chemoproteomic profiling of O‐GlcNAcylated proteins and identification of O‐GlcNAc transferases in rice
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