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  1. 26601
    “…Unusual protein-like, partially reversible associative behaviour has recently been observed in solutions of the water soluble carbohydrates known as 6-deoxy-6-(ω-aminoalkyl)aminocelluloses, which produce controllable self-assembling films for enzyme immobilisation and other biotechnological applications. Now, for the first time, we have found a fully reversible self-association (tetramerisation) within this family of polysaccharides. …”
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    Protein–like fully reversible tetramerisation and super-association of an aminocellulose
  2. 26602
    por Oey, Melanie, Ross, Ian L., Hankamer, Ben
    Publicado 2014
    “…Renewable production systems, including photosynthetic microalgal biotechnologies, can produce biomass for foods, fuels and chemical feedstocks and in parallel allow the production of high value protein products, including recombinant proteins. …”
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    Gateway-Assisted Vector Construction to Facilitate Expression of Foreign Proteins in the Chloroplast of Single Celled Algae
  3. 26603
    “…Unfortunately, Escherichia coli, one of the most widely used microorganisms in biotechnological processes, cannot use starch as a carbon source. …”
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    Engineering Escherichia coli K12 MG1655 to use starch
  4. 26604
    por Fan, Lin-Lin, Li, Hong-Ji, Chen, Qi-He
    Publicado 2014
    “…In recent years, ILs have been widely used in biotechnological fields involving whole-cell biotransformations of biodiesel or biomass, and organic compound synthesis with cells. …”
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    Applications and Mechanisms of Ionic Liquids in Whole-Cell Biotransformation
  5. 26605
    “…Real-time amplification and quantification of specific nucleic acid sequences plays a major role in medical and biotechnological applications. In the case of infectious diseases, such as HIV, quantification of the pathogen-load in patient specimens is critical to assess disease progression and effectiveness of drug therapy. …”
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    Nuclemeter: A Reaction-Diffusion Based Method for Quantifying Nucleic Acids Undergoing Enzymatic Amplification
  6. 26606
    “…Here we use next-generation sequencing to produce a draft genome and transcriptome of T. canis to support future biological and biotechnological investigations. This genome is 317 Mb in size, has a repeat content of 13.5% and encodes at least 18,596 protein-coding genes. …”
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    Genetic blueprint of the zoonotic pathogen Toxocara canis
  7. 26607
    “…The soluble hydrogenase I (SHI) from the hyperthermophile Pyrococcus furiosus, a member of the euryarchaeota, has been studied extensively and used in various biotechnological applications. This review summarizes the strategies used in engineering and characterizing three different forms of SHI and the properties of the recombinant enzymes. …”
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    Production and Application of a Soluble Hydrogenase from Pyrococcus furiosus
  8. 26608
    “…pH-dependent antibodies are engineered to release their target at a slightly acidic pH, a property making them suitable for clinical as well as biotechnological applications. Such antibodies were previously obtained by histidine scanning of pre-existing antibodies, a labor-intensive strategy resulting in antibodies that displayed residual binding to their target at pH 6.0. …”
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     De novo isolation of antibodies with pH-dependent binding properties
  9. 26609
    “…In addition to the fact that it represents a potent vector for the cell penetration of structurally diverse therapeutic compounds, MCa also displays several distinguishing features that make it a potential peptide of choice for clinical and biotechnological applications. The aim of the present study was to gain new information about the properties of MCa in vivo in order to delineate the future potential applications of this vector. …”
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    Biodistribution, Stability, and Blood Distribution of the Cell Penetrating Peptide Maurocalcine in Mice
  10. 26610
    “…The synthesis of highly biocompatible polymers is important for modern biotechnologies and medicine. Here, we report a unique process based on a two-step high-pressure ramp (HPR) for the ultrafast and efficient bulk polymerization of 2-(hydroxyethyl)methacrylate (HEMA) at room temperature without photo- and thermal activation or addition of initiator. …”
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    A New Route for High-Purity Organic Materials: High-Pressure-Ramp-Induced Ultrafast Polymerization of 2-(Hydroxyethyl)Methacrylate
  11. 26611
    “…Usually implicated in specific protein-protein interactions, these proteins are readily amenable for design, with promising biotechnological and biomedical applications. Studying repeat protein families presents technical challenges due to the high sequence divergence among the repeating units. …”
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    Structural and Energetic Characterization of the Ankyrin Repeat Protein Family
  12. 26612
    “…This Gram-positive bacterium has now become of industrial importance as an expression host for the overproduction of lipopolysaccharide-free recombinant proteins used as food ingredients, therapeutic proteins and biotechnological enzymes. RESULTS: This paper reports an agmatine-controlled expression (ACE) system for L. lactis, comprising the lactococcal agmatine-sensor/transcriptional activator AguR and its target promoter P(aguB). …”
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    Implementation of the agmatine-controlled expression system for inducible gene expression in Lactococcus lactis
  13. 26613
    “…Similarly, the initial association step between two binding partners often represents a rate-limiting step in biotechnologically relevant reactions. We demonstrate the practical use of an 11-a.a. …”
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    Speeding up biomolecular interactions by molecular sledding
  14. 26614
    “…LAB are Gram-positive bacteria with great biotechnological potential in the food industry. They can produce bacteriocins, which are proteinaceous antimicrobial molecules with a diverse genetic origin, posttranslationally modified or not, that can help the producer organism to outcompete other bacterial species. …”
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    Bacteriocins of lactic acid bacteria: extending the family
  15. 26615
    “…We have specifically highlighted the role that recent biotechnological advancements have to offer for enrichment of its nutritional value and how these developments can commission to the field of nutritional biology by opening new avenues for future research.…”
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    Nutraceutical Value of Finger Millet [Eleusine coracana (L.) Gaertn.], and Their Improvement Using Omics Approaches
  16. 26616
    por de Ollas, Carlos, Dodd, Ian C.
    Publicado 2016
    “…Understanding the physiological significance of this complex regulation offers opportunities to find strategies of drought tolerance that avoid unwanted side effects that limit growth and yield, and may allow biotechnological crop improvement.…”
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    Physiological impacts of ABA–JA interactions under water-limitation
  17. 26617
    “…The active ingredients can be extracted and purified from natural sources (botanicals, herbal extracts, or animals) but can also be obtained biotechnologically by fermentation and cell cultures or by enzymatic synthesis and modification of natural compounds. …”
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    Enzymatic synthesis of bioactive compounds with high potential for cosmeceutical application
  18. 26618
    “…They are also being harnessed to improve microbial biotechnological processes, including biofuel production. …”
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    How to Measure Export via Bacterial Multidrug Resistance Efflux Pumps
  19. 26619
    “…Summary: Atomistic molecular dynamics simulation is a promising technique to investigate the energetics and dynamics in the protein–surface adsorption process which is of high relevance to modern biotechnological applications. To increase the chance of success in simulating the adsorption process, favorable orientations of the protein at the surface must be determined. …”
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    ProtPOS: a python package for the prediction of protein preferred orientation on a surface
  20. 26620
    “…Current progress in computers and biotechnologies allows improving surgical approach and forecasting final result of reconstruction as well. …”
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    Crouzon syndrome: Virtual planning of surgical treatment by application of internal distractors
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