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26661“…There are numerous examples of the development of new biotechnological approaches to plant protection using transgenic plants and exogenous dsRNAs. …”
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26662por Yoo, Yo-Han, Kim, Yu-Jin, Moon, Sunok, Gho, Yun-Shil, Hong, Woo-Jong, Kim, Eui-Jung, Jiang, Xu, Jung, Ki-Hong“…Promoters are key components for the application of biotechnological techniques in crop plants. Reporter genes such as GUS or GFP have been used to test the activity of promoters for diverse applications. …”
Publicado 2020
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26663por Shishido, Tania Keiko, Popin, Rafael Vicentini, Jokela, Jouni, Wahlsten, Matti, Fiore, Marli Fatima, Fewer, David P., Herfindal, Lars, Sivonen, Kaarina“…Cyanobacteria are photosynthetic organisms that produce a large diversity of natural products with interesting bioactivities for biotechnological and pharmaceutical applications. Cyanobacterial extracts exhibit toxicity towards other microorganisms and cancer cells and, therefore, represent a source of potentially novel natural products for drug discovery. …”
Publicado 2019
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26664“…As the previous literature intensively introduced the genetic construction and application of the genome-reduced Escherichia coli strains, the present review focuses the design principles and compares the reduced genome collections from the specific viewpoint of growth, which represents a fundamental property of living cells and is an important feature for their biotechnological application. For the extended simplification of the genomic sequences, the approach of experimental evolution and concern for medium optimization are newly proposed. …”
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26665“…The last decade has seen bacteria at the forefront of biotechnological innovation, with applications including biomolecular computing, living therapeutics, microbiome engineering and microbial factories. …”
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26666“…We show that orthogonal split inteins can be coupled to multiple split transcription factors to implement complex logic circuits in living organisms, and that they can also be used for the in vitro seamless assembly of large repetitive proteins with biotechnological relevance. Our work demonstrates the versatility and vast potential of an expanded library of orthogonal split inteins for their use in the fields of synthetic biology and protein engineering.…”
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26667por Mizushima, Ryota, Inoue, Kanako, Fujiwara, Hideaki, Iwane, Atsuko H., Watanabe, Tomonobu M., Kimura, Atsuomi“…Recently, GVs have gained considerable attention for biotechnological applications as genetically encodable contrast agents for MRI and ultrasonography. …”
Publicado 2020
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26668“…This opens the possibility for biotechnological production of Tα1 for the research and clinical applications.…”
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26669“…The efficacy of gaseous disinfection is critical for prevention and treatment of microbial contamination in biotechnological facilities. For an evaluation of gaseous disinfection efficacy, a down-scaled laboratory model was established, using currently available carrier tests and a custom-made dry fog box. …”
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26670por Ryan, Jeffrey R.“…Fortunately, the same advances in genomic biotechnologies that can be used to create bioweapons can also be used to set up countermeasures against them. …”
Publicado 2016
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26671por Ciavatta, Maria Letizia, Lefranc, Florence, Vieira, Leandro M., Kiss, Robert, Carbone, Marianna, van Otterlo, Willem A. L., Lopanik, Nicole B., Waeschenbach, Andrea“…It additionally weighs the pros and cons of total syntheses of some bryozoan metabolites versus the synthesis of non-natural analogues, and explores the hopes put into the development of biotechnological approaches to provide sustainable amounts of bryozoan metabolites without harming the natural environment.…”
Publicado 2020
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26672por Rotondo, Cinzia, Corrado, Addolorata, Mansueto, Natalia, Cici, Daniela, Corsi, Fabrizio, Pennella, Antonio, Paolo Cantatore, Francesco“…Moreover, we discuss the clinical manifestation and the therapeutic choices in PWCD, from classical immunosuppressive drugs to new biotechnological agents, and we provide a comprehensive review of the available literature.…”
Publicado 2020
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26673“…The modular structure of MoBPS allows to combine rather coarse simulations, as needed to generate founder populations, with a very detailed modeling of todays’ complex breeding programs, making use of all available biotechnologies. MoBPS provides pre-implemented functions for common breeding practices such as optimum genetic contributions and single-step GBLUP but also allows the user to replace certain steps with personalized and/or self-written solutions.…”
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26674Development and Optimization of a Germination Assay and Long-Term Storage for Cannabis sativa Pollenpor Gaudet, Daniel, Yadav, Narendra Singh, Sorokin, Aleksei, Bilichak, Andriy, Kovalchuk, Igor“…Pollen viability and storage is of great interest to cannabis breeders and researchers to maintain desirable germplasm for future use in breeding or for biotechnological and gene editing applications. Here, we report a simple and efficient cryopreservation method for long-term storage of Cannabis sativa pollen. …”
Publicado 2020
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26675“…Therefore, we hypothesized that bypassing this step by genetically fusing the subunits could be beneficial for biotechnological production of fatty acids. To test the concept, we expressed fused FAS subunits engineered for production of octanoic acid in Saccharomyces cerevisiae. …”
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26676“…Highly stable enzymes exhibiting several industrial and biotechnological properties are being isolated and purified from these extremophiles. …”
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26677“…To reduce the environmental burden, food researchers are seeking strategies to utilize this waste for microbial pigments production and further biotechnological exploitation in functional foods or value-added products. …”
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26678“…Thus, the unique properties of bacillary proteinases, such as stability in a wide range of temperatures and pH, high specificity, biodegradability of a wide range of substrates, and the high potential of sequenced Bacillus genomes are a powerful foundation for the development of new biotechnologies. The current review aims to discuss recent studies on various enzymes in particular, proteinases produced by bacteria of the genus Bacillus, along with their prospective practical applications. …”
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26679“…The design of effective biotechnological processes with this cell factory requires an in-depth knowledge of its metabolism. …”
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26680“…Spatiotemporal control of integrin‐mediated cell adhesions to extracellular matrix regulates cell behavior with has numerous implications for biotechnological applications. In this work, two approaches for regulating cell adhesions in space and time with high precision are reported, both of which utilize green light. …”
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