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29141“…In addition to structural and biochemical data which revealed the archaellum to be a type IV pilus-like structure repurposed as a rotating nanomachine (Beeby et al. 2020), we also review the initial discoveries and subsequent advances using a wide variety of approaches to reveal: complex regulatory events that lead to the assembly of the archaellum filaments (archaellation); the roles of the various archaellum proteins; key post-translational modifications of the archaellum structural subunits; evolutionary relationships; functions of archaella other than motility and the biotechnological potential of this fascinating structure. …”
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29142“…The developed strategy for MMP-9(CAT) stabilization could be applied to redesign of other proteases to improve their stability for various biotechnological applications.…”
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29143“…Indeed, the chitinolytic pathway is initiated by the coexpression of the enzymes endochitinase (EnCh) and chitobiosidase (ChB); however, few studies, including biotechnological production of these enzymes, have been reported, although chitosaccharide are helpful in several industries, such as cosmetics. …”
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29144por Tassara, Eleonora, Orel, Boaz, Ilan, Micha, Cavallo, Dario, Dodero, Andrea, Castellano, Maila, Vicini, Silvia, Giovine, Marco, Pozzolini, Marina“…Chondrosia reniformis (Nardo, 1847) is a marine sponge of high biotechnological interest both for its natural compound content and for its peculiar collagen, which is suitable for the production of innovative biomaterials in the form, for instance, of 2D membranes and hydrogels, exploitable in the fields of tissue engineering and regenerative medicine. …”
Publicado 2023
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29145por Feng, Linjuan, Xu, Junhao, Ye, Cuifang, Gao, Jucan, Huang, Lei, Xu, Zhinan, Lian, Jiazhang“…Triacetic acid lactone (TAL) is a promising renewable platform polyketide with broad biotechnological applications. In this study, we constructed an engineered Pichia pastoris strain for the production of TAL. …”
Publicado 2023
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29146por Johnson, Ryan E., Murray, Makay T., Bycraft, Lucas J., Wetmore, Stacey D., Manderville, Richard A.“…Development of synthetically simple, high yielding modular methods to fine-tune dye performance is crucial to expand the biotechnological applications of oligonucleotides. Herein, we report the utility of 6-hydroxy-indanone (6HI) with a glycol backbone to serve as a handle for on-strand aldehyde capture as a modular aldol approach for site-specific insertion of internal FMR chalcones. …”
Publicado 2023
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29147por Gonçalves, Cleidiane Gonçalves e, Lourenço, Lúcia de Fátima Henriques, Philippsen, Hellen Kempfer, Santos, Alberdan Silva, dos Santos, Lucely Nogueira, Ferreira, Nelson Rosa“…It is, therefore, a hydrolysis process with great biotechnological relevance. Thus, this study aims to use a crude enzyme concentrate (C(EC)) produced by a filamentous fungus to obtain oligomers with different molecular weights. …”
Publicado 2023
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29148por de la Fuente, M. Carmen, Ageitos, Lucía, Lages, Marta A., Martínez-Matamoros, Diana, Forero, Abel M., Balado, Miguel, Lemos, Manuel L., Rodríguez, Jaime, Jiménez, Carlos“…The results obtained will be of great help in the development of biotechnological applications for these types of compounds.…”
Publicado 2023
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29149por Pereira, Bruna Medeiros, Arraes, Fabrício, Martins, Andressa Cunha Quintana, Alves, Nayara Sabrina Freitas, Melo, Bruno Paes, Morgante, Carolina Vianna, Saraiva, Mario Alfredo Passos, Grossi-de-Sá, Maria Fátima, Guimaraes, Patricia Messenberg, Brasileiro, Ana Cristina Miranda“…The established detached-leaf method was further explored for functional validation of two candidate genes encoding for cell wall modifying proteins (CWMPs) to promote resistance against M. incognita through distinct biotechnological strategies: the overexpression of a wild Arachis α-expansin transgene (AdEXPA24) and the dsRNA-mediated silencing of an endogenous soybean polygalacturonase gene (GmPG). …”
Publicado 2023
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29150por Perozeni, Federico, Pivato, Matteo, Angelini, Margherita, Maricchiolo, Elisa, Pompa, Andrea, Ballottari, Matteo“…By utilizing the biotechnological tools available for this green alga, we introduced a synthetic gene encoding a chimeric protein, zeolin, obtained by merging the γ-zein and phaseolin proteins, in the algal genome. …”
Publicado 2023
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29151“…Subsequently, this study comprehensively examines different frameworks and biotechnological techniques available for eliminating heavy metals according to environmental guidelines, underscoring the difficulties and limitations of phytoremediation and its potential application in the clean-up of other harmful pollutants. …”
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29152“…COS-induced salt resilience suggests that COS could become a useful biotechnological tool in reclaiming saline soil for improved crop productivity, especially when such soil is unfit for leading food crops. …”
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29153por Mi, Yidong, Xu, Chao, Li, Xinru, Zhou, Min, Cao, Ke, Dong, Cuimin, Li, Xuemei, Ji, Ningning, Wang, Fanfan, Su, Hailei, Liu, Xuesong, Wei, Yuan“…INTRODUCTION: The lack of systematic investigations of arbuscular mycorrhizal fungi (AMF) community composition is an obstacle to AMF biotechnological applications in antimony (Sb)- and arsenic (As)-polluted soil. …”
Publicado 2023
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29154“…Moreover, it could enable the development of new functional foods and processing strategies to maintain and enhance their physiological benefits, besides the studies of new raw materials for extraction and biotechnological development.…”
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29155por Faria-Reis, Allana, Santos-Araújo, Samara, Pereira, Jéssica, Rios, Thamara, Majerowicz, David, Gondim, Katia C., Ramos, Isabela“…Therefore, it is strategically critical to find molecular targets with biotechnological potential through the functional study of genes essential for insect reproduction. …”
Publicado 2023
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29156por Berlemont, Renaud“…Glycoside hydrolases (GHs) are carbohydrate-active enzymes essential for many environmental (e.g., carbon cycling) and biotechnological (e.g., biofuels) processes. The complete processing of carbohydrates by bacteria requires many enzymes acting synergistically. …”
Publicado 2023
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29157por Wu, Zhirui, Zhang, Tingyue, Li, Jinna, Chen, Sixue, Grin, Inga R., Zharkov, Dmitry O., Yu, Bing, Li, Haiying“…The result has laid a foundation for further mechanistic study of the BvWD40 genes in sugar beet tolerance to salt stress, and it may inform biotechnological applications in improving crop stress resilience.…”
Publicado 2023
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29158por Koehle, Allison P., Brumwell, Stephanie L., Seto, Emily P., Lynch, Anne M., Urbaniak, Camilla“…In this review, information will be presented on the beneficial use of microbes in bioregenerative life support systems, their applicability to bISRU, and their capability to be genetically engineered for biotechnological space applications. In addition, we discuss the negative effect microbes and microbial communities may have on long-duration space travel and provide mitigation strategies to reduce their impact. …”
Publicado 2023
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29159por Kilic, Nur Melis, Singh, Sima, Keles, Gulsu, Cinti, Stefano, Kurbanoglu, Sevinc, Odaci, Dilek“…Moreover, using biosensors to quantify biological or biochemical processes is critical in medical, biological, and biotechnological applications. Nowadays, there are several electrochemical biosensors for various healthcare applications, such as for the determination of glucose, lactate, catecholamines, nucleic acid, uric acid, and so on. …”
Publicado 2023
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29160“…Lipid monolayers are ubiquitous in biological systems and have multiple roles in biotechnological applications, such as lipid coatings that enhance colloidal stability or prevent surface fouling. …”
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