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  1. 30261
    “…The use of transgenic plants to produce novel products has great biotechnological potential as the relatively inexpensive inputs of light, water, and nutrients are utilised in return for potentially valuable bioactive metabolites, diagnostic proteins and vaccines. …”
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    An Interspecific Nicotiana Hybrid as a Useful and Cost-Effective Platform for Production of Animal Vaccines
  2. 30262
    “…Studies with the biotechnological fungal workhorse Trichoderma reesei (Hypocrea jecorina) have revealed an interconnection between transcriptional regulation of cellulolytic enzymes and the light response. …”
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    Unravelling the molecular basis for light modulated cellulase gene expression - the role of photoreceptors in Neurospora crassa
  3. 30263
    “…Furthermore, genome analysis of multiple phototrophic strains allows us to obtain a detailed picture of metabolic diversity that can serve as a starting point for biotechnological applications and automated metabolic reconstructions.…”
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    The diversity of cyanobacterial metabolism: genome analysis of multiple phototrophic microorganisms
  4. 30264
    “…Due to the low content, there is a need to enhance the production of asiaticoside of C. asiatica. The biotechnological approach is one of the methods that can be used to enhance its production. …”
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    Effect of Agrobacterium rhizogenes and elicitation on the asiaticoside production in cell cultures of Centella asiatica
  5. 30265
    “…Protein promiscuity is of considerable interest due its role in adaptive metabolic plasticity, its fundamental connection with molecular evolution and also because of its biotechnological applications. Current views on the relation between primary and promiscuous protein activities stem largely from laboratory evolution experiments aimed at increasing promiscuous activity levels. …”
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    Probing the Mutational Interplay between Primary and Promiscuous Protein Functions: A Computational-Experimental Approach
  6. 30266
    “…It demonstrates that the library of standard biological parts serves as a valuable resource for initial synthetic blocks that needs to be further refined to be successfully applied in practical problems of biotechnological significance. Finally, the development of a predictive model in conjunction with experimental validation facilitates a better understanding of the underlying dynamics and can be used as a guide to experimental design.…”
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    A synthetic biology approach to self-regulatory recombinant protein production in Escherichia coli
  7. 30267
    “…The cellulosome was first discovered in the cellulolytic thermophile, Clostridium thermocellum, and much of our knowledge of this intriguing type of protein composite is based on the cellulosome of this environmentally and biotechnologically important bacterium. The recently sequenced genome of the cellulolytic mesophile, Acetivibrio cellulolyticus, allows detailed comparison of the cellulosomes of these two select cellulosome-producing bacteria. …”
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    Genome-wide analysis of acetivibrio cellulolyticus provides a blueprint of an elaborate cellulosome system
  8. 30268
    “…Our systematic analysis of the GmIPT and GmCKX families has provided an insight into CK metabolism in soybean under drought stress and a solid foundation for in-depth characterization and future development of improved drought-tolerant soybean cultivars by manipulation of CK levels via biotechnological approach.…”
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    Identification and Expression Analysis of Cytokinin Metabolic Genes in Soybean under Normal and Drought Conditions in Relation to Cytokinin Levels
  9. 30269
    “…BACKGROUND: Isobutanol is considered as a leading candidate for the replacement of current fossil fuels, and expected to be produced biotechnologically. Owing to the valuable features, Bacillus subtilis has been engineered as an isobutanol producer, whereas it needs to be further optimized for more efficient production. …”
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    Rational improvement of the engineered isobutanol-producing Bacillus subtilis by elementary mode analysis
  10. 30270
    “…KivRFp has been shown to have useful properties, such as moderate thermal stability and broad pH optima, and may serve as the starting points for future protein engineering and directed evolution, towards the goal of developing efficient enzyme for downstream biotechnological applications.…”
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    Cloning and characterization of a novel 2-ketoisovalerate reductase from the beauvericin producer Fusarium proliferatum LF061
  11. 30271
    “…The rational design of synthetic cell populations with prescribed behaviours is a long-standing goal of synthetic biology, with the potential to greatly accelerate the development of biotechnological applications in areas ranging from medical research to energy production. …”
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    Towards the rational design of synthetic cells with prescribed population dynamics
  12. 30272
    “…Triacylglycerol lipases (EC 3.1.1.3) catalyze both hydrolysis and synthesis reactions with a broad spectrum of substrates rendering them especially suitable for many biotechnological applications. Most lipases used today originate from mesophilic organisms and are susceptible to thermal denaturation whereas only few possess high thermotolerance. …”
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    The Metagenome-Derived Enzymes LipS and LipT Increase the Diversity of Known Lipases
  13. 30273
    “…Overall the study has identified features responsible for the thermostability and enantioselectivity of LinA-type2 that can be exploited for the design of variants for specific biotechnological applications.…”
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    Crystal Structure of the Hexachlorocyclohexane Dehydrochlorinase (LinA-Type2): Mutational Analysis, Thermostability and Enantioselectivity
  14. 30274
    “…These are of most interest for the design of metabolic engineering/synthetic biology strategies in the field of biotechnological applications of this enzyme. RESULTS: We report here the cloning of an aryl-alcohol dehydrogenase cDNA from the white-rot fungus Phanerochaete chrysosporium, its expression in Escherichia coli and the biochemical characterization of the encoded GST and His(6) tagged protein. …”
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    Cloning, expression and characterization of an aryl-alcohol dehydrogenase from the white-rot fungus Phanerochaete chrysosporium strain BKM-F-1767
  15. 30275
    “…Strain tolerance to toxic metabolites is an important trait for many biotechnological applications, such as the production of solvents as biofuels or commodity chemicals. …”
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    Toward a Semisynthetic Stress Response System To Engineer Microbial Solvent Tolerance
  16. 30276
    “…Lactococcus lactis is a biotechnological workhorse for food fermentations and potentially therapeutic products and is therefore widely consumed by humans. …”
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    Mobile CRISPR/Cas-Mediated Bacteriophage Resistance in Lactococcus lactis
  17. 30277
    “…Among them, oxygen sensitivity of hydrogenases represents one of the major limitations hampering the biotechnological implementation of photobiological production processes. …”
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    Reversible oxygen-tolerant hydrogenase carried by free-living N(2)-fixing bacteria isolated from the rhizospheres of rice, maize, and wheat
  18. 30278
    “…Pseudomonas stutzeri A15 is an interesting candidate host for environmentally relevant biotechnological applications. With the recently characterized P. stutzeri A15 EstA autotransporter at hand, all tools for developing a surface display system for environmental use are available. …”
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    Probing the applicability of autotransporter based surface display with the EstA autotransporter of Pseudomonas stutzeri A15
  19. 30279
    “…METHODS: The expression of p42.3 gene was analyzed by RT-PCR, Western Blot and other biotechnologies. The relationship between the spatial conformation of p42.3 protein molecule and its function was analyzed using bioinformatics, MATLAB and related knowledge about protein structure and function. …”
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    p42.3 gene expression in gastric cancer cell and its protein regulatory network analysis
  20. 30280
    “…Our method is of immediate practical relevance for medical and industrial applications, such as the identification of novel drug targets, and the development of biotechnological processes that use complex, largely uncharacterized media, such as biofuel production.…”
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    Inferring Metabolic States in Uncharacterized Environments Using Gene-Expression Measurements
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